Radical and Cationic Pathways in C(sp3)–H Bond Oxygenation by Dioxiranes of Bicyclic and Spirocyclic Hydrocarbons Bearing Cyclopropane Moieties

A product and DFT computational study on the reactions of 3-ethyl-3-(trifluoromethyl)dioxirane (ETFDO) with bicyclic and spirocyclic hydrocarbons bearing cyclopropyl groups was carried out. With bicyclo[n.1.0]alkanes (n = 3–6), diastereoselective formation of the alcohol product derived from C2–H bond hydroxylation was observed, accompanied by smaller amounts of products derived from oxygenation at other sites. With 1-methylbicyclo[4.1.0]heptane, rearranged products were also observed in addition to the unrearranged products deriving from oxygenation at the most activated C2–H and C5–H bonds. With spiro[2.5]octane and 6-tert-butylspiro[2.5]octane, reaction with ETFDO occurred predominantly or exclusively at the axial C4–H to give unrearranged oxygenation products, accompanied by smaller amounts of rearranged bicyclo[4.2.0]octan-1-ols. The good to outstanding site-selectivities and diastereoselectivities are paralleled by the calculated activation free energies for the corresponding reaction pathways. Computations show that the σ* orbitals of the bicyclo[n.1.0]alkane cis or trans C2–H bonds and spiro[2.5]octanes axial C4–H bond hyperconjugatively interact with the Walsh orbitals of the cyclopropane ring, activating these bonds toward HAT to ETFDO. The detection of rearranged oxygenation products in the oxidation of 1-methylbicyclo[4.1.0]heptane, spiro[2.5]octane, and 6-tert-butylspiro[2.5]octane provides unambiguous evidence for the involvement of cationic intermediates in these reactions, representing the first examples on the operation of ET pathways in dioxirane-mediated C(sp3)–H bond oxygenations. Computations support these findings, showing that formation of cationic intermediates is associated with specific stabilizing hyperconjugative interactions between the incipient carbon radical and the cyclopropane C–C bonding orbitals that trigger ET to the incipient dioxirane derived 1,1,1-trifluoro-2-hydroxy-2-butoxyl radical.


■ INTRODUCTION
The cyclopropyl group is an important and versatile motif.Because of its characteristic structural and bonding features, 1 substitution of cyclopropane can modify the properties of substrates and provide access to a variety of useful synthetic transformations.Accordingly, cyclopropane-containing molecules are finding increasing application in organic synthesis, 2 in drug development, 3 and as functional molecules in different fields. 4The cyclopropyl group is also present in several natural products including terpenoids, steroids, and alkaloids, among which, many show biological activity and may serve as potential drug leads. 5 promising approach for structural diversification of cyclopropane containing molecules is represented by C(sp 3 )− H bond functionalization, a mainstream topic of modern synthetic chemistry. 6Overlap between a cyclopropane Walsh C−C bonding orbital and the σ* antibonding orbital of an α-C− H activates this bond toward functionalization (Figure 1a), providing a powerful handle to implement site-selectivity in these reactions.6a Concerted insertion or two-step hydrogen atom transfer (HAT) strategies typically occur.In the latter case, however, because the intermediate cyclopropylcarbinyl radicals formed in the HAT step are known to undergo rapid rearrangement, 7 the procedure is limited to the use of reagents that ensure very fast radical capture, preventing competitive unimolecular pathways and delivering the unrearranged functionalized product.Metaloxo species, 8 dioxiranes, 9 and oxaziridines 10 are examples of such reagents, able to promote stereoretentive C(sp 3 )−H oxygenations.
Along these lines, the C−H bond oxygenation of linear, bicyclic, and spirocyclic substrates bearing cyclopropane moieties has been studied employing a variety of oxygenation reagents. 11High selectivity for hydroxylation and ketonization at the activated α-methylenes over other sites has been generally observed.Similar selectivity patterns have been observed in dihalocarbene insertions into the C(sp 3 )−H bonds of hydrocarbons bearing cyclopropane moieties. 12n the framework of synthetically useful procedures, the full potential of this activation is witnessed by the results obtained by White in the site-selective C−H bond ketonization of a terpenoid derivative with H 2 O 2 catalyzed by the Fe (R,R)-pdp complex, 11f and by Baran in the site-selective and stereoselective C−H bond hydroxylation promoted by 3-methyl-3-(trifluoromethyl)dioxirane (TFDO), employed in an intermediate step of the total synthesis of (+)-phorbol (Figure 1a). 13ecause of the tendency of cyclopropylcarbinyl radicals to undergo rapid rearrangement, 7 cyclopropane-containing substrates are coveted mechanistic probes to study the involvement of radical intermediates in a reaction, 14 to assess the concerted, radical, and/or cationic nature of enzymatic and biomimetic reaction mechanisms, 8a,15 as well as to calibrate the rates of competing radical reactions (Figure 1b).For example, trans-1ethyl-2-phenylcyclopropane has been employed as a probe to calibrate the rate constant for recombination of the radical couple formed in the first step of its reaction with dimethyldioxirane (DMDO). 16Based on a ring-opening rate constant k r = 1 × 10 11 s −1 , and a 40:1 unrearranged/rearranged product ratio, a rate constant k = 4 × 10 12 s −1 could be estimated at room temperature corresponding to a lifetime of the radical couple of 200 fs.
With spiro[2.5]octane, the corresponding cyclopropylcarbinyl radical undergoes ring-opening with k r = 5 × 10 7 s −1 .15a In the framework of the oxygenation of this substrate promoted by metal-oxo species, 11f dioxiranes, 11e ozone, 11a and cytochrome P450 enzymes, 15a no evidence for the formation of products deriving from radical rearrangement has been observed, in line with the relatively low value of k r that prevents competition with the radical capture or radical recombination steps.
With substrates such as spiro[2.5]octaneand bicyclo[4.1.0]heptane(norcarane), the product distribution can also provide information on the possible involvement of cationic intermediates, revealing the occurrence of competitive ET steps.15a In the specific case of spiro[2.5]octane, the formation of bicyclo[4.2.0]octan-1-ol can provide conclusive evidence for the involvement of a cationic intermediate.Evidence for the formation of rearranged alcohol products has been obtained in a recent study on the oxygenation of spiro[2.5]octane and 6-tertbutylspiro[2.5]octanepromoted by manganese-oxo species, where leveraging on the use of fluorinated alcohol solvents and on catalyst electronics, predominant or exclusive formation of bicyclo[4.2.0]octan-1-ol and cis-4-(tert-butyl)-bicyclo [4.2.0]octan-1-ol, respectively, was observed (Scheme 1). 17ecause similar mechanistic features are associated with oxygenations promoted by metal-oxo species and dioxiranes, 8,18 and considering that the oxidizing ability of the intermediate αhydroxy alkoxyl radical formed following HAT to the dioxirane (Scheme 2) can be modulated by careful choice of the precursor ketone as well as by solvent effects, we explored if these reagents in combination with fluorinated alcohol solvents could lead to the (unprecedented) involvement of cationic intermediates in dioxirane reactions.
We report on the results of a detailed product and computational study of the reactions of 3-ethyl-3-(trifluoromethyl)dioxirane (ETFDO) with bicyclic (S1−S5) and spirocyclic (S7 and S8) hydrocarbons bearing cyclopropyl groups, the structures for which are displayed in Figure 2. Product studies have been also extended to 1,1-dimethylcyclohexane (S6) and to the diastereomeric alcohol couples P2a-OH, P2b-OH, P8a-OH, and P8c-OH (Figure 2).
The yield of the minor products deriving from C−H bond oxygenation at remote positions (C-3 for S1 and S2; C-3 and C-4 for S4 and S5) was calculated as the sum of the alcohol and ketone products.In the oxygenation of S3, product yields of alcohols at C-2 and C-5 are given in both cases as the sum of the cis-and trans-isomers (full details on the product distributions are displayed in the SI, Table S3).For the oxidation of S6 and S7, product yields were obtained after chromic acid oxidation of the reaction mixture (see SI, Tables S6 and S7).
The reaction with ETFDO was also extended to some of the oxygenation products of S2 and S8.The main reaction products P2a-OH and P8a-OH and the corresponding ketones P2-O and P8-O were isolated by the scale-up oxidation of S2 and S8, respectively.P2b-OH and P8c-OH (the diastereoisomer of P8a-OH, not observed in the oxidation of S8) were prepared by diastereoselective reduction of parent ketones P2-O and P8-O, respectively (see SI). Conversions and product yields observed in the oxygenation of the isomeric cis-and trans-alcohol products P2a-OH and P2b-OH by ETFDO are displayed in Scheme 5a.The results of the competitive oxygenation of a 1:1 mixture of P2a-OH and P2b-OH by ETFDO are described in Scheme 5b.Scheme 6 shows the conversions and product yields that are observed in the corresponding experiments with P8a-OH and P8c-OH.
Computational Studies.Density functional theory (DFT) computations were performed with Gaussian 16. 20 The ωB97X-D functional was used to optimize molecular geometries, 21 with the 6-311++G(d,p) basis set and the SMD solvation model accounting for H 2 O. 22 Frequency calculations were conducted at the same level of theory used for the geometry optimizations to obtain thermal Gibbs free energies and characterize the stationary points on the potential energy surface.The correct unrestricted wave functions were obtained by performing a stability test with the Gaussian keyword stable = opt.Gibbs free energies were corrected using Goodvibes, which corrects the vibrational frequencies via the approximation for the quasiharmonic correction, as proposed by Grimme. 23Intrinsic reaction coordinate (IRC) calculations were performed to verify that a transition state (TS) connects the reactant and product on the potential energy surface.CYLview was employed to visualize molecular structures. 24he computed site-selectivities for C(sp 3 )−H bond oxygenation of bicyclo[n.1.0]alkanesS1, S2, S4 and S5 with ETFDO are shown in Figure 3.The relative activation free energies (ΔΔG ‡ ) for the C 2 −H and C 3 −H bonds are given in kcal mol −1 .For comparison, the experimental ΔΔG ‡ values, which are derived from the experiments illustrated in Scheme 3 (for which the normalized site-selectivities are displayed in Figure 8), are also shown.
The pertinent transition structures obtained for these selectivity studies together with the analysis of the hyperconjugation effect on the C 2 −H bonds provided by the fused cyclopropane moiety are shown in Figures S7−S10 of the SI for the reactions of substrates S1, S2, S4, and S5, respectively.The computed site-selectivity for the C(sp 3 )−H bond oxygenation of 1-methylbicyclo[4.1.0]heptane(S3) is displayed in Figure 4.
The transition structures for HAT from the C 2 −H and C 5 −H bonds of S3 to ETFDO are displayed in the SI as Figure S11.The energetics of the hydroxylation mechanisms for each of the C−H bonds at C-2 and C-5 are displayed in Figure 5.
The computed site-selectivities for C(sp 3 )−H bond oxygenation of spiro[2.5]octanesS7 and S8 by ETFDO are displayed in Figure 6 along with the experimental ΔΔG ‡ values that are derived from the product distributions displayed in Scheme 4.
The transition structures for HAT from various positions of S7 and S8 to ETFDO and the analysis of the hyperconjugation effect on the C 4 −H bonds provided by the spiro-cyclopropane moiety are displayed in the SI as Figures S12 and S13, respectively.The energetics of the hydroxylation mechanisms for the axial and equatorial C 4 −H bonds of S8 are displayed in Figure 7.
The corresponding energy profiles of the hydroxylation mechanisms for the C 4 −H, C 5 −H, and C 6 −H bonds of S7 are displayed in the SI as Figure S14.products deriving from oxygenation at the other methylenic sites.With all three substrates, no evidence for the formation of the ketone product deriving from overoxidation of the alcohols at C-2, and of products deriving from oxidation of the cyclopropane C−H bonds, was observed.The former observation can be accounted for on the basis of the strong hydrogen bond donor (HBD) ability of HFIP that, by engaging in hydrogen bonding with the hydroxyl group of the alcohol products, inverts the polarity of the adjacent C−H bond, deactivating this site toward HAT to the electrophilic ETFDO. 25The latter observation reflects the very high BDE of the cyclopropane C−H bonds, 26 that are typically resistant to HAT-based functionalization.By increasing the reaction time (48 h for S1, 9 h for S2 and S4), significantly higher conversions were obtained (80−87%), forming substantial amounts of the C-2 ketone.Products are oxygenated at the C-2 position of S1, S2, and S4 with selectivities of 96%, 98%, and 72% respectively.The reaction of S5 was carried out for a 9 h reaction time (86% conversion, 76% overall product yield), with the predominant formation of trans-bicyclo[6.1.0]nonan-2-ol(P5a-OH).These selectivities result from hyperconjugative stabilization, determined by the overlap of a cyclopropane Walsh C−C bonding orbital with the σ* orbital of the adjacent C 2 −H (Figure 1a).6a The analysis of the product distributions obtained for S1, S2, S4, and S5, under conditions where overoxidation is not observed, provides information about the hydroxylation diastereoselectivity.The normalized hydroxylation site-selectivities are displayed in Figure 8.The trans/cis ratios for C 2 −H hydroxylation are highlighted.
Preferential trans C 2 −H hydroxylation was observed for S1, S4, and S5, with the trans/cis ratio that increases with increasing ring size, reaching an upper limit with S5 for which the product deriving from cis C 2 −H hydroxylation was not detected.Preferential cis C 2 −H hydroxylation was instead observed with S2 (trans/cis = 0.14).Interestingly, similar diastereoselectivity patterns were observed in dihalocarbene insertions into the C 2 − H bonds of S1 and S2 (trans/cis = 2.8−4 and 0.23−0.25,respectively), 12 because the same effects operate in dioxirane hydroxylation and carbene insertion reactions.
It is worth noting that cyclopropylcarbinyl stabilization leading to selectivity with S2 also accounts for the diastereoselectivity observed in the oxidation employed in an intermediate step of the total synthesis of (+)-phorbol. 13Within the bicyclo[4.1.0]heptanestructural motif (Figure 1a), selective hydroxylation at the α-C−H bond that is cis to the cyclopropane moiety was observed.
The diastereoselectivities were also explored by computational studies on the oxygenation of S1, S2, S4, and S5 promoted by ETFDO.The activation free energy differences (ΔΔG ‡ ) for HAT from the C 2 −H bonds of these substrates to ETFDO are shown in Figure 3.The corresponding transition structures are presented in the SI (Figures S7−S10).Computational results show a strong preference for the oxygenation of C 2 −H over C 3 − H bonds, supporting the effect of hyperconjugation in C−H bond activation.Moreover, the studies of the oxidation selectivity align with experimental results.Figures S7−S10 highlight the hyperconjugative interaction by the cyclopropyl group when activating the cis and/or trans C 2 −H bond of S1, S2, S4, and S5 toward HAT to ETFDO.
In the reaction of S1, σ* orbitals of both cis and trans C 2 −H bonds can interact with the Walsh orbitals activating these bonds toward HAT.As a result, the energy difference between cis and trans C 2 −H bond oxidation is only 0.5 kcal mol −1 .The effect of hyperconjugation on trans C 2 −H bond activation is highlighted in Figure S7.Experiments did not differentiate the selectivity between cis and trans C 3 −H bonds.However, computations predict a preference for oxygenation of the cis over the trans C 3 − H bond (ΔΔG ‡ = 1.0 and 3.1 kcal mol −1 , respectively).
With S2, the experimental and computational observation of a stronger activation of the cis C 2 −H bond over the trans one is also corroborated by the results obtained, under the same experimental conditions, in the oxidation of cis-and transbicyclo[4.1.0]heptan-2-ol(P2a-OH and P2b-OH, respectively) by ETFDO (Scheme 5a).With both substrates, exclusive formation of the corresponding ketone product (P2-O) in 9.2% and 33% yield, respectively, was observed, indicating that the latter alcohol is 3.6 times more reactive than the former one.P2b-OH displays a cis C 2 −H bond that benefits from hyperconjugative activation, whereas with P2a-OH the trans C 2 −H bond cannot benefit from a similar activation.Additional support is provided by the results obtained in the competitive oxidation of a 1:1 trans−cis mixture of bicyclo[4.1.0]heptan-2ols(P2a-OH and P2b-OH) by ETFDO (Scheme 5b).91% of P2a-OH and 66% of P2b-OH, together with an overall 40% yield of P2-O, were obtained, indicating that the latter alcohol is 3.8 times more reactive than the former one, showing excellent agreement between the two experiments.
Among the bicyclo[n.1.0]alkaneseries, the oxygenation of 1methylbicyclo[4.1.0]heptane(S3) by ETFDO is particularly noteworthy.With this substrate, in addition to the alcohol and ketone products deriving from oxygenation at the most activated C−H bonds at C-2 (P3-OH2 + P3-O2) and C-5 (P3-OH5 + P3-O5) in 33.4% and 32.6% combined yield, respectively, cisand trans-3-methyl-8-oxabicyclo[5.1.0]octan-3-ol(P3-1) were also observed among the reaction products in 13% combined yield (Scheme 3).Full details about the product distribution of this reaction can be found in the SI.The formation of products P3-1 can be rationalized on the basis of the mechanism proposed by Groves and co-workers in the oxygenation of bicyclo[4.1.0]heptane(S2) promoted by cytochrome P450 enzymes.15a The carbon radical formed following HAT from C-2 can undergo, in addition to the canonical OH rebound and radical rearrangement pathways, one-electron oxidation to give a cationic intermediate that, after rearrangement, is converted into the hydroxylated product by OH-transfer or nucleophilic capture by water (Scheme 7).
An analogous mechanism is proposed for the oxidation of S3, where the formation of 1-methylcyclohept-3-en-1-ol is initiated by HAT from the C 5 −H bond.The intermediate alcohol product is then rapidly converted into P3-1 as a diastereomeric mixture via epoxidation by ETFDO. 27This mechanistic
DFT calculations play a pivotal role by providing a qualitative approximation of the reaction outcomes.In a previous study, 18b molecular dynamics revealed a 90% barrierless oxygen-rebound mechanism and 10% radical pair formation, while DFT predicted only a barrierless oxygen-rebound mechanism.This highlights the value of DFT and IRC in capturing the essence of the reaction mechanism, albeit with a degree of approximation.In order to confirm that S8-Int1 is the ion-pair intermediate, the CM5 calculation is employed to check the distribution of charges (Figure 9).The charge is evenly distributed in the 6-tertbutylspiro[2.5]octanyliumcation (+0.94) and trifluoro-2-

Journal of the American Chemical Society
hydroxybutan-2-olate anion (−0.90).Moreover, a hypothetical triplet radical pair S8-Int1a is noticeably unstable compared to ion-pair S8-Int1 by 48.9 kcal mol −1 .An open-shell singlet radical pair is not obtained in the computations with ωB97X-D/6-311+ +G(d,p)/SMD(H 2 O).Open-shell initial guesses led to the closed-shell result.Consequently, a hypothetical triplet radical pair S8-Int1a was employed to compare its energies with the S8-Int1 ion-pair.It is also worth mentioning that formation of a delocalized cation following ET within the hypothetical radical pair S8-Int1a strongly contributes to the reaction exergonicity.Isodesmic reaction calculations show a 10.7 kcal mol −1 thermodynamic advantage for delocalized S8-Int1 over the corresponding localized secondary carbocation (see Table S13 and Scheme S2 in the SI).
Supportive experimental evidence in favor of an ET pathway was gained from the study of the solvent effects on the oxidation reaction.Oxygenation of this substrate by ETFDO was studied in HFIP, TFE, and MeCN.As the solvent HBD ability was reduced, the ratio between rearranged (P8b-OH) and unrearranged (P8a-OH + P8-O) products was diminished, leading to the following P8b-OH/(P8a-OH + P8-O) ratios: 0.065, 0.028, <0.01, for HFIP, TFE, and MeCN, respectively (see SI, Table S10), pointing again toward the ability of fluorinated alcohols to promote ET reactions via an increase in the oxidizing power of ET reagents and to stabilize cationic intermediates. 30,32ased on these mechanistic studies and on previous findings, 17 the oxidation mechanism of S8 by ETFDO is proposed in Scheme 9.An analogous mechanism is found for the oxygenation pathways initiated by HAT from the C 4 −H bond of S7 (Figure S14).
Grabovskiy et al. presented a concerted molecule-induced homolytic/rebound process of cage hydrocarbons using dioxiranes. 33Notably, our findings suggest that the generation of the cationic intermediate is associated with a specific stabilizing hyperconjugative interaction between the incipient carbon radical and the cyclopropane C−C bonding orbitals.This causes ET to the incipient 1,1,1-trifluoro-2-hydroxy-2butoxyl radical. 28he ΔΔG ‡ values for HAT from the C−H bonds of S7 and S8 to ETFDO are displayed in Figure 6.HAT from the C 4 −H bond of S7 presents the lowest energy barrier (ΔΔG ‡ = 0 kcal mol −1 ), in comparison with the energy barriers for C 5 −H and C 6 −H bonds (ΔΔG ‡ = 0.6 and 1.6 kcal mol −1 , respectively).Furthermore, we find that the activation barriers of axial C−H bonds are lower than those of equatorial ones.The transition state structures are shown in Figure S12 in the SI.In S7-TS1 C4ax , hyperconjugation leads to a slightly extended C 1 −C 2 distance (1.52 Å) and a reduced C 1 −C 4 distance (1.49Å), differing from the other transition states that lack Walsh orbital interactions.Moreover, efficient hyperconjugation between the axial C 4 −H bond and the Walsh orbital in the transition state S7-TS1 C4ax is evidenced.
With S8, oxygenation of the axial C 4 −H bond is favored over the equatorial one by 1.9 kcal mol −1 , in good agreement with the experimental studies.Based on the analysis of the transition state structures, a hyperconjugative interaction by cyclopropane Walsh orbitals lowers the barrier of the axial C 4 −H bond.Compared to S8-TS1 eq , S8-TS1 ax exhibits a slightly longer C 1 − C 2 distance (1.52 Å) and a shorter C 1 −C 4 distance (1.49Å) due to hyperconjugation.
The observation of a stronger hyperconjugative activation of the axial C 4 −H bond over the equatorial one is also corroborated by the results obtained, under the same experimental conditions, in the oxidation of trans-and cis-6tert-butylspiro[2.5]octan-4-ol (P8a-OH and P8c-OH, respectively) by ETFDO (Scheme 6a).With both substrates, exclusive formation of the ketone product (P8-O) in 5% and 22% yield, respectively, was observed, indicating that the latter alcohol is 4.4 times more reactive than the former one.P8c-OH displays an axial C 4 −H bond that benefits from hyperconjugative activation, whereas with P8a-OH the equatorial C 4 −H bond For the site-selectivities observed in the reactions of ETFDO with substrates S6−S8, the normalized product distributions are displayed in Figure 10.With S6, comparable selectivities were observed for the three methylenic sites (C-2:C-3:C-4 = 1.0:1.5:1.5).The slightly lower selectivity for oxygenation at C-2 over C-3 and C-4 can be reasonably explained on the basis of steric effects, where the presence of the two methyl groups limits the accessibility of the adjacent C 2 −H bonds to ETFDO.
With S7, taking into account that the rearranged alcohol product P7b-OH derives from initial HAT from the C 4 −H bond, the normalized product distributions (C-4:C-5:C-6 = 7.6:1.0:1.5)point toward a significant activation of the C 4 −H bonds compared to the other methylenic sites.These results are in good agreement with those obtained previously in the oxidation of S7 promoted by the H 2 O 2 /(S,S)-Fe(pdp) and H 2 O 2 /(S,S)-Mn( TIPS pdp) systems and by TFDO.11e,f, 17 As mentioned above, this behavior reflects activation of the axial C 4 −H bonds via overlap with the Walsh C−C cyclopropane bonding orbitals.The site-selectivity observed in the oxygenation of S8, for which exclusive formation of products deriving from initial HAT at this site, reflects the synergistic cooperation of two effects: hyperconjugative C 4 −H bond activation together with C 5 −H and C 6 −H bond deactivation by torsional and steric effects determined by the presence of the bulky tert-butyl group at C-6. 17 The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/jacs.3c07163.Details on the preparation of the substrates, their oxidation reactions by ETFDO, on the isolation and characterization of reaction products, and on the computational studies.(PDF) Scheme 9. Proposed Mechanism for the Oxygenation of S8 Promoted by ETFDO.

Figure 1 .
Figure 1.Use of cyclopropyl containing substrates (a) to induce selectivity in HAT-based C−H bond functionalization procedures and (b) as mechanistic probes.

Figure 4 .
Figure 4. Computed difference in activation free energies (ΔΔG ‡ , in kcal mol −1 ) for HAT from the C 2 −H and C 5 −H bonds in S3 to ETFDO.

Figure 6 .
Figure6.Difference in activation free energies (ΔΔG ‡ , in kcal mol −1 ) for HAT from the C−H bonds of S7 and S8 to ETFDO: computational and experimental studies.
methyl-8-oxabicyclo[5.1.0]octan-3-olsP3-1 by oxygen atom transfer from ETFDO.The proposed mechanistic pathways for oxidation of S3 by EFTDO are summarized in Scheme 8, which shows 3D figures of the intermediate and transition state structures.

Scheme 7 .
Scheme 7. Groves Mechanism for the Oxygenation of S2 Promoted by Cytochrome P450 Enzymes 15a

, 19 ■
CONCLUSIONSThe results of product and computational studies on the C(sp3 )−H bond oxygenation of bicyclic and spirocyclic hydrocarbons bearing cyclopropyl moieties promoted by ETFDO have led to a deeper understanding of the factors that govern selectivity in these processes.Activation of the C−H bonds that are α to the cyclopropyl group occurs when there is strong overlap between the cyclopropane Walsh C−C bonding orbitals and the C−H σ* orbitals.Diastereoselective hydroxylation is typically observed, reflecting preferential activation of one α-C−H bond, with the exclusive detection of a single diastereoisomer in the reactions of bicyclo[6.1.0]nonane(S5) and 6-tert-butylspiro[2.5]octane (S8).The experimental siteselectivities and diastereoselectivities are paralleled by the calculated activation free energies for the corresponding reaction pathways.The detection of rearranged oxygenation products in the oxidation of 1-methylbicyclo[4.1.0]heptane(S3), spiro[2.5]octane(S7), and 6-tert-butylspiro[2.5]octane (S8) provides unambiguous evidence for the involvement of cationic intermediates in these reactions, representing the first examples on the operation of ET pathways in dioxiranemediated C(sp 3 )−H bond oxygenations.34,35With these substrates, calculations predict the direct formation of an intermediate ion pair via HAT from a substrate C−H bond to ETFDO coupled to ET, highlighting the role of specific stabilizing interactions able to assist cation formation and divert the reaction from the canonical HAT/rebound pathway.■ASSOCIATED CONTENT* sı Supporting Information