Evaluation of Solubility Characteristics of a Hybrid Complex of Components of Soy

The purpose of this study was to evaluate the solubilities and physicochemical properties of solid dispersions of daidzein (DDZ) and genistein (GST) (the major isoflavones in soybeans) in γ-cyclodextrin (γCD). Dispersions were prepared in distilled water using a three-dimensional ball mill (3DGMw). Phase solubility diagrams confirmed that DDZ/γCD and GST/γCD formed AL type inclusion complexes with a molar ratio of 1:1. A new peak due to inclusion complexes was observed in the results of powder X-ray diffraction (3DGMw(DDZ/γCD = 1:1) and 3DGMw(GST/γCD = 1:1)). Dissolution tests using distilled water found that solubilities of 3DGMw(DDZ/γCD = 1:1) and 3DGMw(GST/γCD = 1:1) were approximately 37- and 51-fold higher, respectively, than the solubilities of pure DDZ and GST. These observations are expected to expand the usefulness of cogrinding of DDZ or GST with γCD using a three-dimensional ball mill.


INTRODUCTION
Soybeans and soy products are plant-based foods extensively used worldwide. Soy products contain isoflavones, which have structures similar to the female hormone estrogen and which show estrogenic action. 1 Soy isoflavones have been reported to promote osteoplasty 2 and reduce negative symptoms of menopause. 3 Therefore, soy isoflavones are used as dietary supplements or food for specified health promoting effects. Two aglycon-type isoflavones present at relatively high concentrations in soy products are daidzein (DDZ) and genistein (GST). DDZ can reduce the risk of atherosclerotic cardiovascular disease, 4 and GST is useful for chemoprevention of prostatic cancer. 5 The improvement of dispersibility in water and the improvement of bioavailability are required for a more effective use of DDZ and GST since DDZ and GST have poor water solubility, low stability, and low bioavailability.
γ Cyclodextrin (γCD) is a complex cylindrical molecule with eight glucose molecules bonded by α1−4 linkages. Cyclodextrins have cavities in their cyclic structures and are known to form inclusion complexes that harbor other molecules, 6 thereby improving the solubility 7 and stability 8 of various pharmaceuticals. For example, formation of inclusion complexes between γCD and catechins has been reported. 9 Various methods for preparing inclusion complexes, including coprecipitation, 10 kneading, 11 freeze drying, 12 and cogrinding 13 have been reported. Cogrinding is a method of forming inclusion complexes by mechanochemical means under solvent-free conditions, changing the bonding state between two solids and the physicochemical properties of each. 14 Cogrinding methods using a rod mill, a ball mill, a jet mill, and so on have been developed. The preparation of inclusion complexes of caffeic acid/γCD by cogrinding using a rod mill has been reported. 15 Using the ball mill, it is possible to micronize foods and tea. 16 The use of a jet mill for grinding of drugs has been reported. 17 The vibrating rod mill generates heat in the grinding process due to the mechanochemical effect. A ball mill with two-axis rotation is a type of grinder used to grind and blend materials for drug formulation. The three-dimensional (3D) ball mill has longitudinal and transverse rotational axes, enabling mixing and grinding with high uniformity. The preparation of cocrystals of ibuprofen and nicotinamide using a three-dimensional ball mill has been reported. 18 This unique grinding will operate continuously for long periods of time for powder processing. On the other hand, three-dimension ball milling is a novel grinding method that rotates in three axes. The characteristics are that it is possible to grind the drug and the material efficiently and that it does not generate heat during further grinding. In addition, there has been no report on the preparation of a ground mixture by a novel three-dimension ball mill for an inclusion complex of drug and cyclodextrin. Therefore, if it is possible to prepare inclusion complexes by a three-dimensional ball mill, this will lead to expanded application of the pharmaceutical preparation method.
Use of a three-dimensional ball mill may permit the preparation of new types of hybrid solid dispersions.
Inclusion complexes of DDZ/βCD and GST/βCD have been reported. 19 To date, the preparation of inclusion complexes of DDZ and GST using other CDs has not been reported. Therefore, we prepared solid dispersions using γCD with the aim of further improving DDZ and GST solubility.
The aim of the current study was to evaluate the physicochemical properties and solubilities of DDZ/γCD and GST/γCD inclusion complexes prepared by cogrinding using a three-dimensional ball mill.

RESULTS AND DISCUSSION
2.1. Phase Solubility Studies. Phase solubility diagrams were obtained to determine molar ratios and stability constants of DDZ/γCD and GST/γCD inclusion complexes. The solubility of DDZ alone was 1.39 μg/mL and that of GST alone was 2.54 μg/mL after 24 h of shaking. Phase solubility diagrams indicate that the solubilities of DDZ and GST increase linearly with the addition of γCD. The inclusions formed are type A L according to the classification of Higuchi et al. (Figure 1). A L -type inclusions indicate a guest molecule and host molecule interaction with a molar ratio of 1:1. It is suggested that DDZ and GST form 1:1 inclusion complexes with γCD in solution. Stability constant (K) was 220.85 M −1 for DDZ and 1378.12 M −1 for GST. GST is thought to form more stable inclusion complexes within γCD than DDZ since the stability constant of GST is larger than that of DDZ.
2.2. Differential Scanning Calorimetry (DSC). Phase solubility diagrams suggested that DDZ/γCD and GST/γCD complexes had 1:1 molar ratios in the aqueous solution. Solid dispersions of DDZ/γCD and GST/γCD were prepared using a three-dimensional ball mill at molar ratio of 1:1. In most reports, inclusion complex formation changes thermal behaviors, leading to disappearance of or shift in the melting points of guest molecules. 22 DSC was performed to investigate the thermal behavior of 3DGMw(DDZ/γCD) and 3DGMw-(GST/γCD) prepared using a three-dimensional ball mill. Endothermic peaks due to melting were observed at 337°C for DDZ alone and at 301°C for GST alone (Figure 2a,b). In PM(DDZ/γCD) and PM(GST/γCD), DDZ and GST crystals are considered since endothermic peaks from the melting of DDZ and GST were observed at 320 and 299°C, respectively (Figure 2d,e). Endothermic peaks from DDZ and GST were observed at 308°C in GM(DDZ/γCD) and 305°C in GM(GST/γCD) (Figure 2f,g). In 3DGMnw(DDZ/γCD), endothermic peaks from DDZ and GST were confirmed at 313°C in 3DGMnw(DDZ/γCD) and 317°C in 3DGMnw-(GST/γCD) (Figure 2h,i). Endothermic peaks from DDZ and GST disappeared in 3DGMw(DDZ/γCD) and 3DGMw-(GST/γCD) (Figure 2j,k). This data supports the conclusion that DDZ and GST are included within the cavity of γCD in 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD).

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Article were coground using a rod mill did not produce any new peaks. This method apparently lowered inclusion efficiency as grinding produced heat. When dry DDZ and GST were coground with γCD using a three-dimensional ball mill, DDZ and GST did not form inclusion complexes with γCD since they were not adequately dispersed and mixed. However, when DDZ and GST were coground with γCD in distilled water using a three-dimensional ball mill, inclusion complexes formed, as the distilled water promoted entry of DDZ and GST into the cavity of γCD.
2.4. Near-Infrared Absorption Spectroscopy (NIR). The results of DSC and PXRD suggested the possibility that inclusion complexes are forming in 3DGMw. Therefore, NIR measurements were performed to investigate the molecular structure of the complexes in the solid state. DDZ intact produced a peak at 4836 cm −1, corresponding to the hydroxy group derived from its aromatic ring, and a peak at 4456 cm −1 , to corresponding with the alkyl group ( Figure 4A). The peak produced by the hydroxy group attached to its aromatic ring shifts to 4824 cm −1 , and the peak produced by the alkyl group shifts to 4396 cm −1 in GM(DDZ/γCD). The peak produced by the hydroxy group attached to the aromatic ring of DDZ shifts to 4792 cm −1 , and the peak produced by the alkyl group shifts to 4412 cm −1 in 3DGMw(DDZ/γCD). Unground γCD alone produces a peak at 7024 cm −1 , corresponding to its hydroxy group. The peak produced by the hydroxy group derived from γCD shifts to 7008 cm −1 and broadens in GM(DDZ/γCD). This same peak shifts to 7000 cm −1 and broadens in 3DGMw(DDZ/γCD). 3DGMw(DDZ/γCD) was confirmed to broaden this peak more than GM(DDZ/γCD). Finally, unground GST alone produced a peak at 4848 cm −1 , corresponding to the hydroxy group attached to its aromatic ring, and a peak at 4424 cm −1 , corresponding to its alkyl group ( Figure 4B). The peak produced by the hydroxy group on the aromatic ring of GST shifts to 4844 cm −1 , and the peak produced by the alkyl group shifts to 4400 cm −1 in GM(GST/ γCD). The peak produced by the hydroxy group on the aromatic ring of GST shifts to 4792 cm −1 , and the peak produced by the alkyl group shifts to 4408 cm −1 in 3DGMw(GST/γCD). The peak produced by the hydroxy group of γCD shifts to 7012 cm −1 and broadens in

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Article 3DGM(GST/γCD) compared to that of unground γCD alone. This shift and broadening suggest that 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD) have intermolecular hydrogen bonding in the solid state between groups in the cavity of γCD and between groups on DDZ and GST.
2.5. Scanning Electron Microscopy (SEM). The results of DSC and PXRD for 3DGMw(DDZ/γCD) and 3DGMw-(GST/γCD) suggest the formation of an insertion complex. In addition, PXRD confirmed that the crystal state changes upon 3D cogrinding. Therefore, SEM was performed to observe the shape and surface of the crystal formed. DDZ was smooth and had a columnar shape with a length of around 100 μm ( Figure  5A (a)). GST was smooth and had an acicular shape with a length of around 50 μm ( Figure 5B(a)). γCD was angular, smooth, and its particle diameter was around 20 μm ( Figure  5A(b)). In PM(DDZ/γCD) and PM(GST/γCD), we observed crystals similar to those of pure DDZ, GST, and γCD ( Figure 5A(c),B(c)). GM(DDZ/γCD) and GM(GST/ γCD) crystals were 100 μm in diameter, rough, and looked like aggregates of small fragments ( Figure 5A(d),B(d)). 3DGMw-(DDZ/γCD) and 3DGMw(GST/γCD) crystals were 100 μm in diameter, rough, and angular ( Figure 5A(e),B(e)). Generally, inclusions formed by γCD have been described as cubic. 24 Our studies confirmed 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD) crystals to have a cubic shape. It is thought that DDZ and GST are more likely to form inclusion complexes with γCD in the solid state.

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Article infer the relative position of the inclusion complex since it can confirm interactions between the guest molecule and the CD cavity. 3DGMw(DDZ/γCD) produced cross peaks between the H-3 proton (3.75 ppm) in the γCD cavity and the H-a proton (7.7 ppm), the H-e, h proton (7.05 ppm), and the H-d proton (6.3 ppm) of the DDZ ( Figure 6A). In 3DGMw(GST/ γCD), cross peaks were confirmed between the H-3 proton (3.74 ppm) in the γCD cavity and the H-f, g proton (6.7 ppm) and the H-c proton (6.0 ppm) of the GST ( Figure 6B). It is generally known that the H-3 proton is at the wide edge of the CD ring and the H-6 proton is at the narrow edge of the CD ring. Thus, this data suggests the formation of two types of inclusion complexes in which DDZ molecules are enclosed with their phenyl group or their resorcinol moiety facing the narrower edge of the γCD ring (Scheme 1A). Similarly, the data suggests two inclusion types in which GST molecules are enclosed with their phenyl group or their resorcinol moiety facing the narrow edge of the γCD ring (Scheme 1B). It has previously been reported that the DDZ/βCD solid dispersion has two types of inclusion modes. 25 These results suggest that DDZ/γCD and GST/γCD have similar inclusion modes.
2.7. Dissolution Profile (Distilled Water). The results of DSC, PXRD, and NIR suggested the possibility that inclusion complexes formed in the solid state in 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD). Therefore, dissolution tests were performed to investigate whether dissolution behavior of DDZ and GST changed due to inclusion complex formation. The concentration of unground DDZ alone after 5 min was 0.18 μg/mL. The concentration was 0.19 μg/mL for ground DDZ and 1.49 μg/mL for 3D ground DDZ ( Figure 7A). The concentration of unground GST alone after 5 min was 0.19 μg/mL. The concentration was 0.43 μg/mL for ground GST and 0.37 μg/mL for 3D ground GST ( Figure 7B). Concentrations of DDZ were 0.17 μg/mL in PM(DDZ/ γCD), 5.77 μg/mL in GM(DDZ/γCD), and 6.08 μg/mL in 3DGMnw(DDZ/γCD) after 5 min. Both ground mixtures with γCD yielded higher dissolution than DDZ alone. Concentrations of GST were 0.25 μg/mL in PM(GST/γCD), 5.48 μg/mL in GM(GST/γCD), and 3.66 μg/mL in 3DGMnw-(GST/γCD) after 5 min. Concentrations of DDZ and GST in 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD) after 5 min were 6.78 and 8.60 μg/mL. The solubility was notably improved by 3D grinding compared to that of unground DDZ or GST alone. The results of the phase solubility diagrams indicated that the solubility of GST/γCD was higher than that of DDZ/γCD because the solubility of GST alone was higher than that of DDZ alone. Improvements in

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Article solubilities of DDZ in 3DGMw(DDZ/γCD) and GST in 3DGMw(GST/γCD) are attributable to inclusion complex formation. The relatively low solubilities of GM(DDZ/γCD), GM (GST/γCD), 3DGMnw(DDZ/γCD), and 3DGMnw-(GST/γCD) compared to those of 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD) can be explained by failure to form inclusion complexes. This conclusion is supported by our observation of the characteristic diffraction peaks of DDZ and GST alone in the above mixtures in PXRD. Our results indicate that cogrinding in distilled water using a threedimensional ball mill can improve the solubilities of DDZ and GST. The concentrations of DDZ and GST in 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD) after 5 min were 8.91 and 11.43 μg/ mL. Improved dissolution of 3DGMw(DDZ/γCD) and 3DGMw(GST/γCD) in the intestinal fluid was confirmed. CoQ10/γCD inclusion complexes can facilitate CoQ10 incorporation into NaTC when CoQ10/γCD inclusion complexes are dissolved in NaTC solution. CoQ10 can be released in solution from CoQ10/γCD inclusion complexes, and the released CoQ10 forms micelles with NaTC. 26 It has been speculated that DDZ/γCD and GST/γCD inclusion complexes dissolved in FaSSIF solution allow DDZ and GST to be incorporated into NaTC since NaTC is contained in FaSSIF solution. Inclusion complexes prepared for DDZ/βCD with coprecipitation using an organic solvent have been reported. 19 We found that the DDZ or GST/βCD inclusion complex was prepared using a three-dimension ball mill as a new convenient grinding method. We have already reported that the stability constant of the caffeic acid/βCD inclusion complex was higher than that of the caffeic acid/αCD or γCD inclusion complex. 15 If it is possible to prepare for DDZ or GST/αCD orβCD using the three-dimension ball mill method, this would confirm comparing stability constants and differences of solubility with αCB and βCD. Since the solubility of the DDZ or GST/γCD complex is improved under FaSSIF conditions, when an animal experiment was carried out to improve the bioavailability of DDZ and GST might be expected.
In the result of this study, we made a new inclusion complex of DDZ or GST/γCD. Thus, we need to study the difference in the DDZ or GST/CD complex formation between those involving αCD rings and those involving βCD rings.

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Article significantly lower than the IC 50 value for intact GST (201.92 μg/mL).
Electron density increased in the presence of DDZ or GST molecules in the γCD molecule. As a result, the DDZ and GST molecules are more likely to release protons and, subsequently, scavenge DPPH radicals. 21 It is known that the stability constant of an aqueous solution affects its antioxidant capacity. 27 GST/γCD tended to have a higher antioxidant capacity since the results of the phase solubility study indicated that GST/γCD had a higher stability constant than DDZ/ γCD.

CONCLUSIONS
In this study, DDZ/γCD and GST/γCD inclusion complexes were prepared by cogrinding using a three-dimensional ball mill. Structural data on 3DGMw(DDZ/γCD) and 3DGMw-(GST/γCD) inclusion complexes confirmed the formation of two types of complexes in which either the phenyl groups or the resorcinol moieties of DDZ and GST were encapsulated. Improved solubilities of 3DGMw(DDZ/γCD) and 3DGMw-(GST/γCD) due to inclusion complex formation were confirmed. Improved solubilities of DDZ/γCD and GST/ γCD complexes in FaSSIF indicated that the impact of inclusion complex formation will be physiologically relevant in vivo. These results are expected to expand the use of threedimensional ball mill grinding beyond its application to mixing DDZ and GST with γCD. The three-dimensional ball mill will play a part in a novel composite preparation method in the pharmaceutical field.

MATERIALS AND METHODS
4.1. Materials. Daidzein (DDZ) (Figure 10a) was purchased from Kokusan Chemical Co., Ltd. Genistein (GST) (Figure 10b) was purchased from Ark Pharm, Inc. γCD (Figure 10c) was a generous gift from CycloChem Bio Co., Ltd. (Tokyo, Japan) and was stored at 40°C and 82% relative humidity for 7 days. All other chemicals were of analytical grade and were purchased from FUJIFILM Wako Pure Chemical Corporation, Tokyo.
4.3. Methods. 4.3.1. Phase Solubility Study. Solubility studies were conducted according to the method of Higuchi and Connors. 20 Ten milliliter of distilled water was added to 20 mg of each of DDZ and GST, yielding supersaturated solutions of each. Concentrations of γCD ranging from 0 to 30 mM were added, and suspensions were obtained by shaking at 100 rpm and 25 ± 0.5°C for 24 h using a constanttemperature shaking culture machine (BR 42 FL, TAITEC Co., Ltd.). Solutions at equilibrium were filtered through 0.2 μm membrane filters (hydrophilic poly(tetrafluoroethylene) type, DISMIC), and solution concentrations were quantitated. Apparent stability constants (K s ) of DDZ/γCD and GST/γCD inclusion complexes were calculated from slopes of phase solubility diagrams, and solubilities of DDZ and GST in the absence of γCD (S 0 ) were determined using eq 1

Quantitation of DDZ and GST by
High-Performance Liquid Chromatography (HPLC). Solubility was quantified by high-performance liquid chromatography (HPLC: LC-20ADvp, SHIMADZU CORPORATION) using an Inertsil ODS-3 (4.6 × 150 mm 2 , Φ5 μm) column and a detection wavelength of 250 nm. The sample injection volume was 30 μL, and the column temperature was 40°C. The mobile phase for DDZ consisted of acetonitrile/distilled water/acetic acid (25:67.5:7.5). The DDZ retention time was adjusted to 6 min. The mobile phase for GST consisted of acetonitrile/methanol/distilled water/acetic acid (30:15:55:0.1), and the GST retention time was adjusted to 6 min. 4.3.3. Differential Scanning Calorimetry (DSC). The thermal behavior of samples was recorded using a differential scanning calorimeter (Thermo plus EVO, Rigaku Co, Tokyo). All samples were weighed (2 mg) and heated and scanned at a rate of 10.0°C/min under a nitrogen flow (60 mL/min). Aluminum crimp pans were used for all samples.  ACS Omega Article 4.3.4. Powder X-ray Diffraction (PXRD). A MiniFlex II powder X-ray diffractometer (Rigaku Corporation, Tokyo) was used to perform PXRD. The diffraction intensity was measured using a NaI scintillation counter. PXRD was performed using Cu Kα radiation (30 kV, 15 mA), a scan rate of 4°/min, and a scan range of 2θ = 5−40°. Powder samples were held between glass plates to yield a flat sample plane when measurements were performed. 4.3.5. Near-Infrared (NIR) Absorption Spectroscopy. Fourier transform near-infrared absorption spectroscopy (Buchi NIR Flex N-500: Nihon Buchi) was used to perform NIR. Measurement conditions were as follows: wave number was 10 000−4000 cm −1 , scan time was 8 s, and scan temperature was 40°C. Each sample was placed into a sample cup, and measurements were performed at intervals of 1 nm on the optical path.
4.3.6. Scanning Electron Microscopy (SEM). An S3000 N scanning electron microscope (Hitachi High-Technologies Corporation) was used to perform SEM. Gold was deposited on each sample for 70 s, and microscopy was performed at an accelerating voltage of 10 kV.
4.3.7. Measurement of 1 H− 1 H Nuclear Overhauser Effect Spectroscopy (NOESY) NMR Spectra. A 700 MHz NMR system (Agilent Technologies) was used to perform NMR. The solvent was D 2 O. The resonance frequency was 699.6 MHz, pulse width was 45°, relaxation delay was 1.500 s, and temperature was 25°C with 256 increments.
4.3.8. Dissolution Profile. Dissolution tests were performed using the paddle method of the JP17 revised dissolution test on an NTR-593 dissolution apparatus (Toyama Sangyo Co. Ltd., Japan). Nine hundred milliliter of distilled water (37 ± 0.5°C) and 300 mL of fasted state simulated intestinal fluid (FaSSIF) (37 ± 0.5°C) were stirred at 50 rpm. Ten milligrams of each of DDZ and GST were accurately weighed and added to the paddle apparatus. Ten milliliter of each dissolved sample was collected at 5, 10, 15, 30, 60, and 120 min and filtered through 0.2 μm membrane filters. To keep the total solution volume constant, an equal volume of solution held at the same temperature was added to the paddle apparatus after each sample was collected. Quantitation was performed by the same method as was used in phase solubility studies.
4.3.9. Preparation of Fasted State Simulated Intestinal Fluid (FaSSIF). To prepare 500 mL of FaSSIF, 500 mL of the stock solution was used to dissolve sodium dihydrogen phosphate to a final concentration of 31.526 mM and sodium chloride to a final concentration of 116.6 mM. The pH was adjusted to 6.5 with sodium hydroxide. Sodium taurocholate (NaTC) was dissolved to a final concentration of 3 mM using 125 mL of stock solution. Biochemistry-grade lecithin (0.75 mM) was dissolved in 3 mL of dichloromethane in a recovery flask. The NaTC solution (125 mL) was added to the lecithin solution while applying ultrasound. The resulting solution was depressurized with a rotary evaporator (Rotavapor R-215, Nihon Buchi) at 40°C for 15 min at 100 mbar. Once the white turbid solution became clear, if the odor of dichloromethane was detected, vacuum was applied for 5 min at 50 mbar. The depressurized solution was put into a 500 mL volumetric flask. The recovery flask was washed with the remaining 325 mL of stock solution, which was then added to the volumetric flask. Subsequent mixtures were prepared in the same 500 mL volumetric flask using a portion of the solution to wash the other recovery flasks and containers used to prepare the FaSSIF solution.

DPPH Radical Scavenging Test.
A DPPH radical scavenging test was performed using a Spectra Max 190 microplate reader (Molecular Devices Japan Co., Ltd., Tokyo, Japan). Each sample was dissolved in equal volumes of methanol and phosphate buffer, pH 6.8 (second fluid in the revised JP17 dissolution test [second fluid]), and then filtered through 0.2 μm membrane filters. A DPPH/methanol solution (500 μm) was mixed in a microplate at a volume ratio of 4:1. The mixture was then incubated at 37°C for 50 min while being shielded from light, and the absorbance (As) of DPPH was measured at a wavelength of 517 nm. A mixture of methanol/second fluid/DPPH methanol (2:2:1) with a rate of radical removal of 0% (A 0 ) and a mixture of methanol/second fluid (3:2) with a rate of radical removal of 100% (Blank, Bl) were prepared. The radical scavenging rate was calculated using eq 2 21 4.3.11. Statistical Analysis. Data are expressed as the mean ± standard deviation (SD). Groups were compared using oneway analysis of variance followed by Tukey's test for multiple comparison. p < 0.01 was considered statistically significant.