Cu(II) and Cd(II) Removal Efficiency of Microbially Redox-Activated Magnetite Nanoparticles

Heavy metal pollutants in the environment are of global concern due to their risk of contaminating drinking water and food supplies. Removal of these metals can be achieved by adsorption to mixed-valent magnetite nanoparticles (MNPs) due to their high surface area, reactivity, and ability for magnetic recovery. The adsorption capacity and overall efficiency of MNPs are influenced by redox state as well as surface charge, the latter of which is directly related to solution pH. However, the influence of microbial redox cycling of iron (Fe) in magnetite alongside the change of pH on the metal adsorption process by MNPs remains an open question. Here we investigated adsorption of Cd2+ and Cu2+ by MNPs at different pH values that were modified by microbial Fe(II) oxidation or Fe(III) reduction. We found that the maximum adsorption capacity increased with pH for Cd2+ from 256 μmol/g Fe at pH 5.0 to 478 μmol/g Fe at pH 7.3 and for Cu2+ from 229 μmol/g Fe at pH 5.0 to 274 μmol/g Fe at pH 5.5. Microbially reduced MNPs exhibited the greatest adsorption for both Cu2+ and Cd2+ (632 μmol/g Fe at pH 7.3 for Cd2+ and 530 μmol/g Fe at pH 5.5 for Cu2+). Magnetite oxidation also enhanced adsorption of Cu2+ but inhibited Cd2+. Our results show that microbial modification of MNPs has an important impact on the (im-)mobilization of aqueous contaminations like Cu2+ and Cd2+ and that a change in stoichiometry of the MNPs can have a greater influence than a change of pH.


■ INTRODUCTION
Advancements in industrialization and agriculture have led to increasing heavy metal concentrations in the environment, causing concerns about drinking water quality. 1 Widespread use of cadmium (Cd) in industrial processes such as battery manufacturing and of copper (Cu) in plumbing resulted in increased concentrations of these contaminants in the environment. 2,3Removal of these contaminants to achieve safe drinking water and maintain fertile soil is of high interest and continuous investigation. 1,4Prolonged ingestion of increased concentrations of heavy metals can lead to adverse effects.Cd is a heavy metal without known metabolic function and is toxic even in very low concentrations. 5In addition to battery manufacturing and combustion, Cd is widespread as a contaminant in agricultural phosphorus-based fertilizers. 6,7Cd is considered carcinogenic, and prolonged exposure to Cd can lead to kidney diseases. 5In contrast to Cd, Cu is an essential trace metal, but high concentrations have been associated with liver damage and possibly gastrointestinal diseases in humans. 8igh Cu concentrations cause oxidative stress through reactive oxygen species on a molecular level. 9Cu is introduced into the environment through industry and in vineyards and orchards where it is used as a fungicide. 10Cu and Cd are not biodegradable, accumulate in the environment, and ultimately end up in bodies of water.
A range of techniques such as membrane filtration and ion exchange are used to treat heavy metal pollutions. 11dsorption is a frequently used method for heavy metal removal due to the relative simplicity of implementation and economic efficiency. 12,13Iron(III) (Fe(III)) (oxyhydr)oxides are commonly used as adsorbents to remove contaminants from solution and are used commercially. 14In Vietnam the precipitation of Fe(III) (oxyhydr)oxides in household sand filters has been shown to be highly effective at removing dissolved toxic arsenic (As). 15,16Fe oxides generally have a high surface area and reactivity, which makes them an ideal adsorbent material. 17Magnetite is a naturally occurring mixedvalent Fe oxide that contains both Fe(II) and Fe(III) (Fe(III) 2 Fe(II)O 4 ).It can be formed abiotically through weathering 18 and biologically through dissimilatory Fe(III) reduction 19 and oxidation. 20,21Magnetite nanoparticles (MNPs) especially can be applied in heavy metal remediation since they have high specific surface area, redox reactivity and can be magnetically extracted.Recent studies investigated the adsorption of chromium (Cr) and As by bioengineered magnetite 22 and the removal of Cr by magnetite-coated sand. 23,24Due to its multivalent nature, unlike most other iron oxides, magnetite can be both oxidized and reduced via microbial activity of Fe(II)-oxidizing and Fe(III)-reducing bacteria, respectively.This was previously shown for the photoautotrophic Fe(II)-oxidizing bacteria Rhodopseudomonas palustris TIE-1 and Fe(III)-reducing bacteria Geobacter sulfurreducens. 25Changing the Fe(II)/Fe(III) ratio in magnetite can ultimately lead to its dissolution through reductive dissolution or transformation to maghemite (maghemitization) through oxidation. 26,27However, magnetite can have a wide range of Fe(II)/Fe(III) ratios while not undergoing transformation to a different mineral and maintaining the crystal structure of magnetite. 25,28The change of the stoichiometry in MNPs can greatly improve the remediation capacity of magnetite, which was previously shown for Cr. 29,30Conversely, it has also been shown that microbial activity decreased the reactivity of MNPs toward As(V) 22 and that magnetite surface passivation can occur through chromium reduction to Cr(III), resulting in a surface layer maghemitization. 31Studies have shown that increase of Fe 2+ led to greater reduction of nitroaromatic compounds 32 and that an increased stoichiometry in magnetite enhanced the capacity to bind antibiotics. 33dditionally, the recharging of magnetite with Fe 2+ for increased reactivity has been demonstrated. 34Previous research investigating removal of Cu 2+ with magnetite mainly focused on the adsorption process without accounting for the Fe(II)/Fe(III) ratio of magnetite or modified particles with magnetite to obtain magnetic removal. 35,36The stoichiometry however directly influences the surface properties of MNPs which are also a consequence of the pH value of the solution.
In this study we consider the impact of microbially mediated redox reactions on the reactivity of MNPs toward two divalent heavy metals.In particular we oxidized MNPs by the autotrophic nitrate-reducing Fe(II)-oxidizing culture KS, 37,38 reduced magnetite by the Fe(III)-reducing bacterium G. sulfurreducens, and compared the adsorption of Cu 2+ and Cd 2+ against unaltered (native) MNPs.We also tested how changes in pH influence adsorption to the three types of MNPs.The results presented below consider both adsorption isotherms and kinetic experiments of Cd 2+ and Cu 2+ on oxidized mag ox , reduced mag red , and native mag nat MNPs.

Safety Statement.
No unexpected or unusually high safety hazards were encountered during experiments performed for this research.
Preparation of Solutions.For all adsorption experiments, anoxic stock solutions of the adsorbent (mag ox , mag red , or mag nat ), adsorbate (CuNO 3 or CdNO 3 ), and solvent (0.1 M NaNO 3 ) were adjusted to the desired pH 2 days prior to the start of the experiment.pH was adjusted with diluted puriss.HNO 3 and NaOH.The pH was checked at least twice per day and corrected accordingly.All solutions were prepared with ultrapure H 2 O (Milli-Q, Merck Milli-pore).Glassware and rubber stoppers were soaked for 10 min with 1 M HCl and then rinsed 3 times with Milli-Q-H 2 O.
Magnetite Synthesis, Oxidation, Reduction, and Stoichiometry.Magnetite was produced according to Pearce et.al 39 but modified to allow magnetite synthesis outside of the glovebox and on a larger scale.For oxidation, magnetite was incubated with the autotrophic nitrate-reducing iron-oxidizing culture KS as previously described 40 with 4 mM NaNO 3 for 7 days, with an increased inoculum of 10% v/v.We previously detected that culture KS can oxidize magnetite.For reduction, magnetite was incubated with 10% v/v of iron-reducing G. sulfurreducens with 20 mM sodium acetate for 5 days. 25After incubation magnetite was washed at least 5 times with 0.1 M NaNO 3 to remove all cells, and minerals were collected with a strong bar magnet after each washing step.Magnetite stoichiometry was measured by the ferrozine assay 41 adapted to microtiter plates.Removal of biomass was checked by measuring DOC (dissolved organic carbon) (High TOC II, Elementar, Elementar Analysensysteme GmbH, Germany) of a washed sample and via fluorescence microscopy by applying a dead/live stain (BacLight Bacterial Viability Kits, Molecular Probes) to screen for any leftover cells after the washing procedure.
Adsorption Isotherms.All experiments were set up in an anoxic glovebox.Triplicate bottles of increasing concentrations of Cu 2+ or Cd 2+ and controls (no MNPs/no adsorbate) were prepared by adding anoxic stock solutions of NaNO 3 followed by well-mixed MNPs and then Cu 2+ /Cd 2+ to obtain a total volume of 5 mL in each bottle.The final concentration of magnetite was 9 mM (as total Fe).Concentration of adsorbate depended on the conducted experiment.The bottles were sealed with rubber stoppers, mixed, and then incubated in the dark at 25 °C on a rolling shaker.After 24 h of incubation the bottles were sampled in the glovebox.Two milliliters was removed with a pipet and centrifuged for 2 min at 10 000g, and the sample then was split into pellet and supernatant fractions.Outside of the glovebox, the pellet was dissolved in 2 mL of 6 M puriss.HCl for 15 min.Supernatant and dissolved pellet were diluted in 2% puriss.HNO 3 and measured with microwave plasma-atomic excitation spectroscopy (Agilent 4200 MP-AES, Agilent Technologies).In total, 12 isotherms were obtained for the following: Cd 2+ + mag nat at pH 5.0, pH 5.5, 6.5, and 7.3; Cd 2+ + mag ox and Cd 2+ + mag red at pH 5.5 and 7.3; Cu 2+ + mag nat at pH 5.0 and 5.5; Cu 2 + mag ox and Cu 2+ + mag red at pH 5.5.Experiments with Cu 2+ were only conducted at pH 5.0 and 5.5.The pH was chosen to avoid precipitation of Cu(OH) 2 which occurs for concentrations of 2 mM (as present in starting stock solutions) above pH 5.53, with the solubility product of Cu-hydroxide being K sp (Cu-(OH) 2 ) = 2.20 −20 .While precipitation is a method for remediation purposes, this study focused on adsorption from solution to the magnetite surface, and hence the pH values were not higher than 5.5 for Cu 2+ .
Kinetic Adsorption Experiments.For kinetic adsorption experiments, different treatments were prepared as above, in triplicate in the glovebox, but with a total volume of 50 mL.For each time point 2 mL of well-mixed liquid was removed, centrifuged for 2 min at 10 000g, and then further treated as described above to separate aqueous and solid fractions.Kinetic experiments were performed with 500 μM Cd 2+ at pH 5.5 and 7.3 for all types of MNPs.For Cu 2+ , 750 μM was utilized at pH 5.0 with native MNPs only and at pH 5.5 with all types of MNPs.The different initial concentrations of Cd 2+ and Cu 2+ were selected based upon their respective adsorption isotherms that led to approximately 50% adsorption in the respective pH ranges.
Metal Analyses.Concentrations of Cd, Cu, and Fe were determined with MP-AES, equipped with a SP3 autosampler.Samples were diluted in 2% puriss.HNO 3 to obtain a concentration in the measurement range of the instrument.The measurement wavelengths were 371.993 nm for Fe, 228.802 nm for Cd, and 324.754 nm for Cu.The obtained data were first processed by the internal software of the instrument (MP Expert software, 1.5.0.6545).
Specific Surface Area.Magnetite nanoparticles were anoxically freeze-dried and weighed anoxically, and then the specific surface area (SSA) was quantified with a Micromeritics Gemini VII surface area and porosity analyzer (Micromeritics Instrument Cooperation, USA), equipped with a VacPrep 061 and using N 2 as adsorbate.SSA was only determined for mag nat particles.
Mossbauer Spectroscopy.One sample of the native magnetite was filtered in a glovebox through a 0.45 μm poresize syringe filter (Millipore membrane), embedded in Kapton tape and stored at −20 °C until measurement.The sample was inserted into a closed-cycle exchange gas cryostat (SHI-650-5; Janis Research, USA).The spectrum was collected at 140 K using a constant acceleration drive system (WissEI, Blieskastel, Germany).γ-Radiation was emitted by a 57 Co-source embedded in a rhodium matrix.The sample spectrum was calibrated against a 7 μm thick Fe(0) foil at room temperature.The software package recoil (University of Ottawa, Canada) was used for fitting using the extended Voigt-based fitting model.The Lorentzian half-width−half-maximum (hwhm) value was kept constant at 0.124 mm/s.The spectrum was analyzed with respect to the isomer shift (δ), the quadrupole splitting (ΔE Q ), and the hyperfine magnetic field (B hf ), and the Gaussian width (standard deviation) of the ΔE Q was used to account for line broadening until the fit was reasonable.
Micro X-ray Diffraction.Samples for micro X-ray diffraction (μ-XRD) were washed with anoxic Milli-Q and anoxically dried in an Eppendorf tube in the glovebox.μ-XRD was performed with a Bruker's D8 Discover GADDS XRD2 microdiffractometer equipped with a standard sealed tube with a Cu-cathode (Cu Kα radiation, λ = 0.154 nm, 30 kV/30 mA).The total measurement time was 240 s at two detector positions, 15°and 40°.Phase identification was validated using Match!software version 3.7.1.123with Crystallography Open Database (COD-Inorg REV211633 2018.19.25).μ-XRD patterns were utilized to obtain information about mineralogy and crystal size.The Scherrer equation (eq 1) was applied to calculate average crystal size d: with K = shape factor (0.9), λ = wavelength of the source, β = full width at half-maximum (fwhm), and cos θ the cosine of the Bragg angle θ.Data Treatment and Models of Isotherm and Kinetic Adsorption.The data obtained from MP-AES measurements were evaluated to obtain the amount of adsorbed contaminant as Cu/Cd in μmol on mass of Fe in g (μmol/g Fe) by calculating mean and standard deviation of technical triplicates.We used both Langmuir 43 and Freundlich 44 isotherms (eqs 2 and 3) for all collected data sets.
c s,i (μmol/g) represents the amount of adsorbed Cd 2+ or Cu 2+ , c w,i is the concentration in solution (μmol/L), k ads,i (μmol/L) is the binding constant, and q max,i (μmol/g) is the maximum adsorption capacity.k i is the Freundlich adsorption coefficient [(μmol/g)(L/g) n ], and n is the Freundlich coefficient.Here the subscript i always refers to the different experiments (pH/ magnetite/heavy metal).Isotherms were fit using the nonlinear least-squares solver lsqnonlin (trust region approach) 23,45 in MATLAB (R2022b) (objective function in Parameter estimation).For all the parametrizations we report the fitted parameter values and the goodness of fit of the model as normalized root-mean-square-error (NRMSE) (eq 4) 46 where n is the number of observations and i the observation indices.
For kinetic experiments, the rates of adsorption of Cu 2+ and Cd 2+ were defined by a linear driving force 24,44 and a secondorder adsorption scheme (eqs 5 and 6). 24Divalent heavy metals (HM(II)) were assumed to be distributed between equilibrium S HM(II) EQ and actual concentration of adsorbed Cu 2+ or Cd 2+ (S HM(II) ) (μmol/g).This approach was previously utilized. 24,47Here, we applied both Langmuir and Freundlich isotherms (eqs 2 and 3) to compute the equilibrium concentration S HM(II) EQ .The rates of adsorption were finally formulated by multiplying the concentration differences by the empirical kinetic adsorption rates constants k sorb,1 (s −1 ) and k sorb,2 (μmol −1 g s −1 ) for eqs 5 and 6, respectively.
The ordinary differential equation (ODE) (eq 7) was solved in MATLAB using the ODE solver ode15s. 48arameter Estimation.The model (eqs 2, 3, and 5−7) parameters q max , k ads , k, n, k sorb,1 , and k sorb,2 were estimated.The objective function is defined in eq 8 where θ is the parameter vector and y obs,i the observations.The lsqnonlin algorithm in MATLAB was used for optimization by minimizing eq 8. NRMSE was computed to evaluate the goodness of fit (eq 4).
■ RESULTS AND DISCUSSION Magnetite Characterization.Synthesized native magnetite mag nat had a Fe(II)/Fe(III) ratio of 0.42 ± 0.01.Microbially oxidized (mag ox ) and reduced (mag red ) magnetite had ratios of 0.26 ± 0.02 and 0.54 ± 0.03 respectively, suggesting successful magnetite oxidation and reduction by the nitrate-reducing Fe(II)-oxidizing culture KS and Fe(III)-reducer G. sulfurreducens.The SSA of the freeze-dried MNPs measured with BET was 92.73 m 2 /g, which was comparable with literature. 39The high SSA is explained by the small size of the particles, as the described synthesis method commonly results in particles in a size order of 10 nm. 39Calculated apparent diameter d app 49 resulted in 12.49 nm. 57Fe Mossbauer analysis at 140 K confirmed that the prepared mineral was magnetite with two characteristic sextets in the spectrum correlating to Fe in octahedral and Fe in tetrahedral coordination (Figure S1 and Table S1).The Fe(II)/Fe(III) ratio was calculated according to Gorski and Scherer 27 as 0.46 ± 0.024, which was in reasonable agreement with the ratio determined by the ferrozine assay (0.42 ± 0.01).
μ-XRD also confirmed materials used for all adsorption experiments to be magnetite (Figures S2−S4 -rich medium used in this study. 50,51Based on the relatively low intensity of the reflections in the XRD patterns, coupled to previous measurements of the SSA of vivianite of 8−16 m 2 /g, 52,53 we anticipate that the effect of vivianite in this system was minor and did not influence the adsorption experiments.The Scherrer equation (eq 1) was used to calculate the average crystal size 42,54 of 9.59 nm for mag red and 10.23 nm for mag ox and 10.29 nm for mag nat .The slight decrease of crystal size for the reduced MNPs reflected a relative change of 6.8% (0.699 nm) and of 0.53% (0.055 mm) for mag ox .μ-XRD patterns were collected for native MNPs after kinetic and isotherm experiments, with all results confirming pure magnetite and no vivianite (Figures S3 and  S4).
Using the average crystal size obtained from the Scherrer equation, we calculated the theoretical SSA according to Etique et al. 49 to be 107.7 m 2 g −1 for mag red , 101.0 m 2 g −1 for mag ox , and 100.4 m 2 g −1 for mag nat .This suggests microbial activity influenced the SSA of the MNPs, though the differences are relatively small.Comparison to the BETresults, the measured SSA (92.73 m 2 g −1 ) for mag nat showed that the measurement and calculation are within 10% relative error.Since our calculated SSAs showed small differences overall of less than 7%, the great changes of adsorption properties cannot be explained by the changes in surface area alone.
To confirm the successful removal of biomass, the DOC content of the supernatant of the washed particles was determined.The results yielded a DOC content of 1.28 mg C/L which is just slightly above the Milli-Q water used to prepare all solutions (0.95 mg C/L).Representative fluorescence microscopy images, collected after washing oxidized and reduced MNPs 5 times (Figures S5 and S6), showed no more colored areas, suggesting successful removal of cells.Figure S7 shows the results after washing the reduced MNPs only once which shows many cells remained associated with the MNPs.
While we made every effort to wash the MNPs to be free from bacteria, we cannot guarantee that no residual organic compounds remained.The NO 3 − anion is however not expected to have a significant influence on the magnetite properties because it has been shown before that the binding of metals with nitrate is minor or negligible 55 and the adsorption of nitrate to magnetite is minor. 56,57We therefore propose that any influence of NO 3 − on the adsorption of metal cations was systematic and not significant.
Since this study is dealing with adsorption of Cu and Cd onto nanoparticles, particle aggregation is an important process 58,59 that could influence the available surface area and thus adsorption capacities.If any organic compounds (from biomass) remained in the magnetite solution after washing, particle aggregation could have influenced 60 the adsorption.In a previous study 61 the comparison of abiotically synthesized and biologically induced MNPs showed aggregation differences between biogenic MNPs (larger and less compact).However, in our study the microorganisms were not responsible for the synthesis of the MNPs, and as shown above, our MNPs were thoroughly washed and showed little evidence of any associated organic compounds, suggesting that its impact on aggregation and adsorption itself should be minor.
Redox Potential and pH PZC .Gorski et al. 62 empirically derived a linear relationship of the Fe(II)/Fe(III) ratio in magnetite and its open circuit potential (E OCP ).They showed that an increase in the stoichiometry of magnetite resulted in a decrease of E OCP .Using this expression, we calculated the potential of our MNPs which resulted in −0.54, −0.36, and −0.12 mV for mag red , mag nat , and mag ox respectively.This suggests that the potential in our MNPs changed over ±0.42 mV from oxidized to reduced magnetite.
Literature described the point of zero charge pH PZC for magnetite at around pH 6.5. 17,63Therefore, we can assume that at pH 5.5, 6.5, and 7.3 mag nat should have positive, almost neutral, and negatively charged surface potential at the three different pH values, respectively.We can therefore assume that the pH PZC shifted relatively toward lower pH values for mag red and toward higher pH values for mag ox .
Adsorption Isotherms and Kinetics.i. Copper.For mag nat , the maximum concentration of adsorbed Cu 2+ increased from 228.69 ± 6.25 μmol/g Fe (pH 5.0) to 273.9 ± 6.32 μmol/g Fe (pH 5.5) (Figure 1).Adsorption experiments with oxidized and reduced magnetite were conducted with Cu 2+ at pH 5.5.Mag ox at pH 5.5 exhibited similar adsorption that was slightly increased (286.44 ± 8.01 μmol Cu/g Fe) over mag nat (273.9 ± 6.32 μmol Cu/g Fe), indicating the effect of microbial oxidation of magnetite was minor.In stark contrast, mag red adsorbed 530.13 ± 14.70 μmol/g Fe, which was roughly twice as much as for mag nat and mag ox .Reduction of magnetite has been previously described to "charge" particles with electrons 28 for both nano-and microscaled particles.This could lead to a corresponding increase in "negative charge" and decrease the point of zero charge of the magnetite and ultimately lead to a less positively charged surface.The point of zero charge (pH PZC ) is defined as the pH where the total net charge on the surface is zero 17 as discussed above.Below the pH PZC , the electrostatic repulsion effect of the same charges, here the positively charged surface of MNPs and divalent cation (Cu 2+ ), decreased as the surface sites of magnetite deviated from a fully protonated surface (−FeOH 2 + ) toward a more negatively charged surface (−FeOH − ). 17The more negatively charged the surface, the more positively charged Cu 2+ can adsorb.Alternatively, the increased adsorption capacity could be due to an increased SSA as a result of microbially induced dissolution.Without further measurements these assumptions are however only speculative, and we suggest that both mechanisms occurred.
Kinetic adsorption experiments were carried out to better understand the time dependence of Cu 2+ adsorption to the different types of magnetite.Mag nat was tested at pH 5.0 and 5.5 with little divergence in the concentration of Cu 2+ adsorption until the final sampling time point at 24 h (Figure S8).It was expected that an increased pH would lead to increased adsorption, since the surface charge of the mineral was less negative. 17he adsorption on mag red after 5 min was already 40 μmol/g Fe greater than that on mag ox and 146 μmol/g Fe greater than that on mag nat (Figure 2).After 1 day 429.56 ± 4.05 μmol Cu/ g Fe was adsorbed on mag red , 286.79 ± 2.97 μmol Cu/g Fe on mag ox , and 222.23 ± 9.60 μmol Cu/g Fe on mag nat .The adsorption of Cu 2+ on MNPs did not reach equilibrium after 24 h for intermediate and higher concentrations of dissolved Cu 2+ , as adsorption continued onto mag red/ox between hours 26.75 and 37.75.The difference after a few minutes of contact time shows the importance of the stoichiometry of the MNPs (changed through microbial oxidation and reduction) on the rate of adsorption.Both mag ox and mag red adsorbed twice as much Cu 2+ as mag nat immediately and showed higher capacity even after 2 days.
ii. Cadmium.Since Cd 2+ is more soluble than Cu 2+ across a wide pH range, Cd 2+ adsorption isotherms to mag nat were performed at pH 5.0, 5.5, 6.5, and 7.3.As expected, the amount of adsorbed Cd 2+ on native MNPs increased with pH from 256.95 ± 45.68 μmol/g Fe (pH 5.0), 284.97 ± 24.19 μmol/g Fe (pH 5.5), 417.78 ± 16.08 μmol/g Fe (pH 6.5), to 478.20 ± 4.66 μmol/g Fe (pH 7.3) (see Figure S9).Due to the previously discussed change of positive to negative surface charge across the point of zero charge, more Cd 2+ was able to adsorb on the native MNPs with increasing pH.Plotting the maximum of adsorbed Cd 2+ vs pH (see Figure S10) reveals a linear relationship in the observed pH range.We assumed that further increasing pH will lead to more adsorption of Cd 2+ onto MNPs.Based on a dissolved Cd 2+ concentration of 1.5 mM, this shows that adsorption could be studied up to pH 8.6 without precipitation of cadmium hydroxide Cd(OH) 2 (K sp of Cd(OH) 2 ) = 2.5 −14 ).Using the linear trend shown in Figured S10, we calculated the maximum possible amount of Cd on mag nat under these conditions as 610.66 μmol Cd/g Fe.
Isotherm (Figure 1) and kinetic (Figure 3) experiments were performed at pH 5.5 and 7.3 for native, oxidized, and reduced MNPs.At pH 5.5 mag ox could adsorb less Cd 2+ (239.84 ± 1.54 μmol Cd/g Fe) compared to mag red (299.68 ± 8.31 μmol Cd/g Fe), which was slightly above mag nat (284.97 ± 24.19 μmol Cd/g Fe) but within standard deviation of the mean.When comparing results at pH 5.5 for mag nat , mag red , and mag ox , the change in stoichiometry, especially when MNPs were reduced, showed a much greater effect for Cu 2+ than Cd 2+ .Even though both Cu 2+ and Cd 2+ are divalent cations, Cd 2+ has a much bigger radius of 109 pm, while the Cu 2+ radius is only 87 pm.−66 At pH 7.3, mag ox showed the lowest removal capacity toward Cd 2+ with 351.19 ± 1.14 μmol Cd/g Fe, followed by mag nat with 478.20 ± 4.66 μmol Cd/g Fe, and surpassed by mag red with 631.72 ± 11.00 μmol Cd/g Fe.The increased pH led to a less positively charged surface area, and hence more divalent  cations could adsorb.Interestingly, at pH 7.3 the stoichiometry of MNPs had a greater influence than at pH 5.5 as seen by the greater adsorption by mag red , which was also reflected in the difference between maximum adsorption of Cd at pH 5.5 and pH 7.3 (Figure 4).The increase of adsorbed Cd 2+ from pH 5.5 to 7.3 was 111.36 μmol Cd/g Fe for mag ox , 193.23 μmol Cd/g Fe for mag nat , and 332.04 μmol Cd/g Fe for mag red .At higher pH both the Fe(II)-enriched negatively charged magnetite surface area and the more negatively charged bulk mineral yielded higher Cd 2+ adsorption.Independently of pH the oxidation of MNPs showed a decrease in adsorption capacity toward Cd 2+ .We suggest that the increase in positive charge of the MNPs exhibits a repulsive force on the Cd 2+ ions.The change in stoichiometry of the MNPs played an important role at high pH values for Cd 2+ , while the effect of pH dominated at pH 5.5 and an influence of the stoichiometry could still be detected at pH 5.5 that resulted in increased adsorption for mag red and decreased adsorption for mag ox .
Results from the kinetic experiments for Cd 2+ ([Cd 2+ ] = 500 μM) performed with all MNPs at pH 5.5 and 7.3 confirmed the previously discussed findings and expanded on them (Figure 3).At both pH values, the adsorption of Cd 2+ on mag ox showed the slowest rate and achieved the lowest total amount after more than 2 days.Rate and amount of adsorbed Cd 2+ on reduced MNPs were greater when compared to native MNPs.Interestingly, the amount of adsorbed Cd 2+ at this intermediate concentration in solution (initially 500 μM Cd 2+ ) on mag red at pH 5.5 reached roughly the same value (333.84 ± 4.85 μmol Cd/g Fe) after only 67 h as the adsorbed Cd 2+ on mag nat at pH 7.3 after 96 h (335.35 ± 4.23 μmol Cd/g Fe).This showed that for Cd 2+ adsorption on MNP, the reduction led to an increase of the adsorption rate and capacity.Our results showed the same trends for pH 7.3.Mag ox adsorption was smallest, followed by mag nat , and then surpassed by mag red .We can see in Figure 3 that the amount of adsorbed Cd 2+ on mag nat after 12 h did not increase much further, while the amount on mag red continued to increase until the last sampling time point.This suggests that independent of the pH the oxidation of MNPs greatly hinders the adsorption of Cd 2+ while reduction greatly increased it.At low pH there was little difference in the performance of mag nat or mag ox with respect to Cd 2+ .However, almost immediately 161.15 μmol Cd/g Fe was adsorbed by mag red (Figure 3), which was 1.4× as much as mag nat with 114.88 μmol Cd/g Fe and 1.7× as much as mag ox with 97.34 μmol Cd/g Fe at the same time point.Therefore, reduced MNPs provide enhanced adsorption even for short contact times and low pH values.Additionally, mag red initially adsorbed 270.86 μmol/g Fe at pH 7.3, which was more than mag ox (184.14 μmol Cd/g Fe) but similar to mag nat (275.16μmol Cd/g Fe).Adsorption to mag ox remained low by the end of the study (242.41μmol Cd/g Fe) whereas adsorption on mag nat increased to 335.35 μmol/g Fe and to 478.15 μmol/g Fe for mag red .
Figure 4 summarizes the maximum measured adsorbed amount of Cd 2+ /Cu 2+ on the different redox MNPs.We show the maximum adsorbed amount for isotherms (left panel, same c w concentration range for Cu 2+ and Cd 2+ ) and the kinetic experiments (right two panels, different c w for Cd 2+ and Cu 2+ during kinetic experiments) but only discuss the numbers of the isotherms and use the kinetic data to support these findings.We can see that for mag nat at pH 5.5 the amount of adsorbed Cu 2+ and Cd 2+ was within standard deviation, suggesting that at this pH all available surface sites of the unaltered magnetite were saturated for both heavy metals.At pH 7.3 (only Cd 2+ ) about 159 additional μmol Cd/g Fe was adsorbed for mag nat , showing the importance of pH for adsorption processes (see also Figure S9).For mag ox , the adsorption of Cu 2+ increased slightly compared to mag nat .More Cu 2+ than Cd 2+ was adsorbed on mag ox , since the amount of adsorbed Cd 2+ slightly decreased from mag nat to mag ox which was in contrast with the slight increase for Cu 2+ .This suggests that previously occupied surfaces sites were not available anymore for Cd 2+ but remained available for Cu 2+ .Additionally, a more positively "redox-discharged" mineral (decreased Fe(II)/Fe(III) ratio) likely increased electrostatic repulsion toward the bigger cation Cd 2+ more profoundly than for smaller Cu 2+ .Previously the oxidation of magnetite was reported as a surface sensitive process, 28,67 and hence this positively charged surface would repel Cd 2+ .This is reflected in the low adsorption of Cd 2+ with mag ox at pH 7.3.These findings were supported by kinetic experiments, which consistently showed smaller c s values for Cd 2+ on mag ox than for mag nat and greater c s values for Cu 2+ with mag ox than for mag nat .For Cd 2+ at pH 7.3, the amount of adsorbed Cd 2+ on mag ox decreased by roughly 127 μmol Cd/g Fe compared to mag nat and conclusively only increased by roughly 66 μmol Cd/g Fe compared to pH 5.5 mag ox , showing the importance of the minerals' stoichiometry.Considering Figure S10, the theoretical maximum c s of Cd 2+ was calculated as 610.60 μmol Cd/g Fe at pH 8.6, just before precipitation of Cd(OH) 2 .At pH 7.3, mag red already showed a higher c s of 631.72 ± 11.00 μmol Cd/g Fe, again emphasizing the great importance of MNPs' stoichiometry.The difference at pH 7.3 between mag nat and mag ox was more profound than for pH 5.5, as more Cd 2+ was adsorbed to the minerals' surface at pH 7.3 to begin with.Additionally, it appears that the impact of surface charge is more important at low pH than the stoichiometry for Cd 2+ and that the stoichiometry gains importance as pH rises.This was also supported by the kinetic experiments (Figure 3) where we consistently measured increasing c s values of Cd 2+ in the order of mag ox < mag nat < mag red .For mag red the amount of adsorbed Cd 2+ at pH 5.5 was within error of mag ox and slightly greater than mag nat , supporting the hypothesis that the adsorption process in the system was mostly influenced by pH.A low pH value led to a positive surface charge of the MNPs, as the pH PZC was previously reported between 6.1 and 8 for magnetite. 17,67Interestingly adsorption of Cu 2+ on mag red was almost doubled to 530 μmol Cu/g Fe (see also Figure 1) compared to mag nat at pH 5.5.It appears that the net negative charge of the "bulk" magnetite 28 influenced the adsorption of the smaller cation Cu 2+ at lower pH more intensely than for the bigger Cd 2+ cation already at pH 5.5.With mag red the amount of adsorbed Cd 2+ only increased within standard deviation at pH 5.5 while the amount of Cd 2+ adsorbed on mag red at pH 7.3 increased to 631.72 ± 11.00 μmol Cd/g Fe, which was 153 μmol Cd/g Fe greater than on mag nat .While the pH had greater influence on the adsorption of Cd 2+ onto the MNPs surface at low pH values for Cd 2+ , increase to pH 7.3 revealed the importance of MNPs' stoichiometry as the adsorption capacity was decreased for mag ox and increased for mag red , both compared to mag nat , which was again consistent with the kinetic data (Figure 3, Figure 4 right panels).It was previously reported 33 that the stoichiometry of magnetite is a key parameter for the binding of emerging organic contaminants and naturally ligands, as they showed for nalixidic acid the importance of redox for removal of Cr and As. 22We add on to this knowledge by showing that the stoichiometry of magnetite is crucial for the removal of different divalent heavy metals and that it can have a greater impact than change of pH.
Importance of Contact Time.While the isotherm experiments with Cu 2+ (Figure 1) indicated that the time frame of 24 h was sufficient, the kinetic experiments revealed that the adsorbed amount of Cu 2+ still increased, especially for mag ox and mag red , even after 42 h (Figure 2).Therefore, a longer contact time would be needed in order to obtain equilibrium.The isotherms collected for Cd 2+ showed that, especially at high pH values and with mag nat and mag red , the contact time of 24 h was insufficient (Figure 1 and Figure 3).As we could show with the kinetics experiment for Cd 2+ at pH 5.5 and 7.3 for all MNPs, a contact time of 24 h was sufficient for mag nat , but at least 48 h was needed for mag red and mag ox .We therefore recommend a contact time greater than 48 h to explore the future potential of microbially enhanced MNPs for heavy metal removal.
Modeling.Kinetic Experiments.The results and corresponding parameters of the kinetic experiments are shown in Figures S11−S12 and Tables S3−S5.Since the experiments with Cu 2+ were performed in a narrow range of pH, all kinetic experiments could be modeled using a first-or second-order rate with a NRMSE < 0.06.Data presented in Figure S11 and Table S3−S5 suggested that the collected data could be fitted well with both Langmuir or Freundlich equilibria and first-or second-order kinetics.However, a second-order scheme seemed slightly more suitable for Cu 2+ with all types of MNPs at both investigated pH values for both equilibrium isotherms (Langmuir or Freundlich).This could indicate that the adsorption of Cu 2+ onto MNPs is governed by a chemisorption process, which would then have been the rate-determining step. 68Previous studies on adsorption of Cu 2+ onto magnetite have reported that second-order kinetics was a superior model. 35For Cd 2+ , no good modeled results were obtained for mag nat at pH 5.5, suggesting that the collected data were of inferior quality compared to the other data set, which could also be implied by (comparatively) large standard deviation of the mean.Additionally, mag nat at pH 7.3 with Cd 2+ also did not yield a good modeled results; while the model parameters could be bent to fit the data (Figure S12), the parameter results presented in Tables S4 and S5 were not reasonable.In summary, there was not a clear trend in favor of one specific model, and hence either Langmuir or Freundlich as first-or second-order kinetics could be used.
Adsorption Isotherms.The results of the modeled isotherms can be seen in Table S2 and in Figure 1.The results suggest that for Cu 2+ a Freundlich model was a better fit for the pH 5.5 isotherms with mag nat while mag ox and mag red were better estimated by a Langmuir equilibrium.Enhanced adsorption due to oxidation and reduction enabled higher c s (adsorbed amount) values which then allowed better estimation of q max .Freundlich isotherms seemed to overestimate concentrations of Cu 2+ , if c w (concentration in solution) would be increased further.For Cd 2+ with mag nat , a Langmuir model fit better but for pH 7.3 a Freundlich isotherm was more appropriate (as seen by NRMSE).For Cd 2+ at pH 5.5 and 7.3 with all types of MNPs, both Langmuir and Freundlich fits were suitable (Figure 1).Most models had a NRMSE of <0.1.Cd 2+ isotherms generally followed a Freundlich model, which showed consistency in increasing k (distribution coefficient) for increasing pH of native magnetite (pH 5.0, 5.5, 6.5, 7.3: 2.52, 4.12, 22.20, 41.72, respectively) and for increasing pH for reduced and oxidized magnetite (pH 5.5 and 7.3, mag red : 28.28, 62.24, mag ox : 20.75, 64.90).Here the model however does not result in appropriate k values, where mag red showed much higher total adsorption than mag ox .This was better modeled following the Langmuir equation, and we obtained appropriate q max values for Cd at pH 7.3: mag red : 663.7 μmol/g Fe and mag ox : 339.7 μmol/g Fe.
Overall, both heavy metals could be characterized by either Langmuir or Freundlich isotherms at equilibrium.Table S2 shows the NRMSE of all experiments.The goodness of fits at different isotherm varied marginally.For the kinetics, both first-and second-order rates were tested with both Langmuir and Freundlich equilibrium assumptions, and all combinations could reproduce the dynamics in the data well (NRMSE in Table S3).Finally, while it depended on the investigated experiment which model fit best, we could parametrize a reasonable model that fits (almost) all data sets.

■ CONCLUSIONS
We investigated native, microbially oxidized and microbially reduced magnetite nanoparticles (MNPs) for the amount and rate of adsorption toward the two divalent heavy metals Cd 2+ and Cu 2+ .Our results presented here show that the influence of microbial oxidation and reduction of Fe in these MNPs greatly influences the adsorption behavior of these environmentally relevant metals.For Cu 2+ we showed that the reduction of MNPs leads to an increase in adsorption capacity.This was expected since the reduction likely led to an increased negative bulk charge of the MNPs as we could show with potential calculations (Table S6).Additionally partial dissolution, as shown by μXRD, led to an increase in SSA of the particles (Table S6).Even the oxidized MNPs showed an increase in adsorption toward dissolved Cu 2+ with respect to native MNPs, a phenomenon that we are unable to fully explain even when considering the slight differences in calculated SSA.As the redox potential of oxidized MNPs is higher, repulsion due to the same charges was expected to be a dominating factor during adsorption.It was assumed that the change in stoichiometry toward Fe(III) (i.e., more positively charged MNPs) would lead to a decrease in adsorption capacity and efficiency through charge repulsion.Our isotherm and kinetic experiments however showed that the opposite is true.Possibly vacancies in the mineral due to reorganization within the crystal structure 26 could have given smaller Cu 2+ ions (87 pm ionic radius) more available adsorption sites.On the other hand, we showed that the increase in Fe(II)/Fe(III) ratio in magnetite due to magnetite reduction resulted in almost 2 times greater adsorption of 663.7 μmol/g Fe than for mag nat .For Cd 2+ , we could see that at low pH values, the stoichiometry of the MNPs had a minor effect on the adsorption behavior, most likely because the greater ionic radius of Cd 2+ (109 pm) was repelled due to the same charge from the positively charged magnetite surface, even if the "bulk" was more negatively charged after reduction.This could explain the minor increase of adsorption of MNPs at pH 5.5 for mag red and the detectable decrease for mag ox .At higher pH, we showed that the oxidation of MNPs led to a more pronounced decreased adsorption capacity and rate even compared to native MNPs.Furthermore, reduction of MNPs led to an increase of adsorbed Cd on mag red compared to mag nat and mag ox .Our results show that ultimately both pH and stoichiometry are highly important parameters for the adsorption processes on MNPs.For relatively small divalent cations like Cu 2+ , stoichiometry had an impact at low pH values, and both microbial oxidation and microbial reduction enhanced the adsorption capacity.For larger ions like Cd 2+ , electrostatic repulsion seemed to be the dominant process at low pH, where stoichiometry mattered less, but oxidation and reduction had great influences at higher pH values.
The MNPs used in this study were cleaned from biomass prior to experiments; however, in nature such "clean" MNPs are not expected to exist.Instead, MNPs are more likely associated with biomass from bacteria (e.g., Fe(II)-oxidizing or Fe(III)-reducing bacteria) or other redox active compounds such as natural organic matter.This associated biomass could potentially have a great influence on the adsorption of Cu 2+ and Cd 2+ by, among other effects, blocking surface sites, 69 changing surface charge, 70 or influencing the particle aggregation. 61Therefore, to better understand the importance of biologically reduced and oxidized MNPs in the environment, further comparative studies should be performed to investigate the role of this naturally occurring biomass and its impact on the ability of bioreduced and bio-oxidized MNPs to adsorb Cu 2+ , Cd 2+ , or other metals.
Finally, our results show that the biomodification of magnetite nanoparticles could be of great use for remediation purposes and drinking water purification.However, it seems that not one material can be applied for all contaminations and all conditions, but that the environment of adsorption (microbial oxidation or reduction) and the pH of the systems must be evaluated and chosen depending on which heavy metal should be remediated most efficiently.
). Minor reflections corresponding to vivianite (Fe II 3 (PO 4 ) 2 , 8H 2 O) were visible in the pattern for mag red at 2Θ of 15.32°.The reduction of magnetite by G. sulfurreducens presumably caused partial dissolution of some Fe 2+ which precipitated as vivianite in the PO 4 3−

Figure 1 .
Figure 1.Measured data and fit isotherms for Cu 2+ (a−c) and Cd 2+ (d−f) adsorption at pH 5.5 (circles) with native (gray), reduced (green), and oxidized (yellow) magnetite nanoparticles.Additionally at pH 7.3 (diamonds) for Cd 2+ .Triplicate bottles with increasing Cu 2+ /Cd 2+ concentrations were incubated for 24 h, and the amount of adsorbed Cu/Cd (in μmol) on mass of magnetite (as g Fe) was determined via MP-AES.Langmuir (orange) and Freundlich (blue) isotherms were modeled.Gray triangles for Cu 2+ with native magnetite show results of isotherm at pH 5.0.

Figure 3 .
Figure 3. Kinetic behavior of Cd 2+ adsorption on magnetite nanoparticles at pH 5.5 (circles) and pH 7.3 (diamonds) with native (gray, panel b), reduced (green, panel c), and oxidized (yellow, panel a) magnetite.Triplicate bottles were incubated with magnetite (as 9 mM Fe) and 500 μM Cd 2+ .Adsorbed Cd (in μmol) on mass of magnetite (as g Fe) was measured via MP-AES.

Figure 4 .
Figure 4. Summary of maximum adsorbed heavy metal concentrations for isotherm experiments (a) and kinetic experiments (b, c) with MNPs.MNPs were untreated (native: MagNat) or microbially oxidized (MagOx) or reduced (MagOx).Displayed are the results as μmol heavy metal/g Fe ± standard deviation for Cd 2+ at pH 5.5 (light green circles) and pH 7.3 (dark gray diamonds) and Cu 2+ at pH 5.5 (red circles).

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ASSOCIATED CONTENT * sı Supporting Information The Supporting Information is available free of charge at h t t p s : / / p u b s .a c s .o r g / d o i / 1 0 . 1 0 2 1 / a c s e a r t h s p a c echem.2c00394.Mossbauer spectrum and fitting results, additional μ-XRD patterns of kinetic experiments, fluorescence microscopy images, kinetic and isotherm figures, linear pH−adsorption fit, Matlab data and model of kinetic experiments and their fitting parameters, and a summary of properties of MNPs (PDF) Cadmium and copper redox magnetite isotherms, cadmium and copper kinetics, μXRD, and Mossbauer data (XLSX) ■ AUTHOR INFORMATION Corresponding Author James M. Byrne − School of Earth Sciences, University of Bristol, BS8 1RJ Bristol, United Kingdom; orcid.org/0000-0002-4399-7336; Email: james.byrne@brystol.ac.uk