ent-Clavilactone J and Its Quinone Derivative, Meroterpenoids from the Fungus Resupinatus sp.

Metabolites 1 and 2, isolated from cultures of the basidiomycete Resupinatus sp. BCC84615, collected in a tropical forest in northeastern Thailand, showed weak antibiotic activity against Bacillus subtilis and Staphylococcus aureus and cytotoxicity against cancer cell lines. Their planar structures were elucidated by high-resolution electrospray ionization mass spectrometry and NMR spectroscopy as clavilactone J, known from the basidiomycete Ampulloclitocybe clavipes, and its new 1,4-benzoquinone derivative. A detailed analysis of the ROESY correlations in 1 confirmed the recent revision of the relative configuration of clavilactone J. However, specific rotation and Cotton effects observed by electronic circular dichroism were contrary to those of the clavilactones; thus, we assigned a rare antipodal absolute configuration.

−6 The present study deals with a Basidiomycota collected from northeastern Thailand and its secondary metabolites.
The strain Resupinatus sp.BCC84615 was identified by a comparison of morphological characteristics and sequencing of the 5.8S/ITS nrDNA region.A BLAST search in GenBank confirmed that the strain belongs to the genus Resupinatus.Based on its basidiocarps (Figure S1) and culture morphology, we classified this fungus as Resupinatus sp.The genus Resupinatus is distinguished by a spore-bearing surface folded into gills (lamellae) and small, virtually black fruiting bodies.Resupinatus and Hohenbuehelia have macromorphologies that are extremely similar.The unique characteristic that separates these two genera is the presence in Hohenbuehelia sp. of a gelatin layer in the cap tissue layer or pileus hymenium layer.This gelatin layer is absent in fungi belonging to Resupinatus.When examined under a microscope, the Thai specimens lacked a gelatin layer, which is consistent with the phylogenetic results indicating that they belong to the same clade as the genus Resupinatus. 7,8Members of Resupinatus have rarely been studied for secondary metabolites, and we know of only two studies describing secondary metabolites from the genus Resupitatus, including two 14-noreudesmane sesquiterpenoids described from R. leightonhenii and a bioactive germacrane sesquiterpene and a nerolidol derivative isolated from R. leightonii. 9,10Extracts of strain BCC84615 exhibited activity against Bacillus subtilis, and therefore, the strain was chosen for a detailed metabolite analysis.
The crude ethyl acetate extract of Resupinatus sp.BCC84615 contained only 1 and 2 as major metabolites (Figure S24), which were isolated subsequently by preparative HPLC.The molecular formula of 1 was deduced from the molecular ion cluster at m/z 305.1020 (M + H) + in the high-resolution electrospray ionization mass spectrometry (HRESIMS) spectrum as C 16 H 16 O 6 , indicating 7 degrees of unsaturation. 1 H and HSQC NMR data showed the presence of two aromatic methines, three oximethines, three methylenes, and one methyl.The 13 C NMR spectrum furthermore indicated one carboxylic carbon (δ C 171.9), four aromatic carbons (δ C 149.1, 148.8, 125.9, and 120.3), and two oxygenated sp 3  (δ H 2.77) and explained the remaining two degrees of unsaturation.Although 1 was not included in the database Dictionary of Natural Products 11 or SciFinder N , 12 the structure of 1 was described by Hou et al. in 2022 as clavilactone J. 13 Very recently, Novitskiy and Kutateladze revised the configuration of clavilactone J using a DFT computational NMR method. 14In our study, we back up this structural revision based on ROESY data.In the ROESY NMR spectrum, key correlations were observed between H-7/H 3 -16 and H-7/ H-11 (Figure 1), confirming a 6R*,7R*,8R*,11R*,12S* relative configuration, since both H-11 and H 3 -16 are oriented on the same face.However, our measured specific rotation, [α] 20 D = −22 (c 0.1, MeOH), was identical in magnitude but opposite in sign to that previously reported ([α] 25 D = +22 (c 0.05, MeOH).Additionally, the electronic circular dichroism (ECD) spectrum of 1 (Figure 2) is a mirror image of that of clavilactone J described by Hou and colleagues. 13Consequently, compound 1 was unambiguously assigned as entclavilactone J.
Metabolite 2 was isolated as a solid with the molecular formula C 16 H 14 O 6 , corresponding to an additional degree of unsaturation.The 1 H and HSQC NMR spectra of 2 were highly similar to those of 1.However, key differences were observed for aromatic ring carbons C-1, C-2, C-3, C-4, C-5, and C-14 in the 13 C NMR spectrum.In particular, the chemical shifts of C-1 and C-4 indicate a para-quinone system.Therefore, 2 was identified as the 1,4-benzoquinone derivative of 1 and named ent-clavilactone J quinone.
The clavilactones are a family of meroterpenoids containing a constrained 10-membered ring fused to a 2,3-epoxy-γ-lactone and a benzoquinone or hydroquinone from cultures of the edible mushroom Ampulloclitocybe clavipes (synonym Clitocybe clavipes). 15,16Since the initial isolation of clavilactones A−C, 17 derivatives clavilactone D, 18,19 E, 20 F, 21 G−I, 22 and J and K 13 have been isolated from the same producing species.The derivatives differ from parental clavilactones A and B by hydroxylation or amination at different locations (Figure S23).To evaluate the taxonomic relationship of strain BCC84615 to Resupinatus and Ampulloclitocybe, a maximum-likelihood phylogenetic tree was generated showing the relationship between Resupinatus and closely related genera like Hohenbuehelia.The Ampulloclitocybe sequence was included to visualize the distance from the genus Resupinatus.In the resulting tree, Resupinatus and Ampullocitocybe are substantially separated, allowing us to use Ampullocitocybe, which is from a different family, as the outgroup for this population's analysis.In other words, 1 and its previously reported enantiomer clavilactone J originate from distinct organism families, a highly surprising result (Figure S2).
In addition to antibacterial and antifungal activities, clavilactones A, B, and D are epidermal growth factor receptor tyrosine kinase inhibitors. 21Furthermore, seco-clavilactone B is an actin polymerization inhibitor. 22or 1, we observed weak antibiotic activity against Grampositive bacteria (S. aureus, B. subtilis) in our panel of test organisms, 23 whereas Gram-negative bacteria and fungi were not affected (Table 1).ent-Clavilactone J, and to a lesser extent, the quinone form, exhibited antiproliferative effects against various cell lines (Table 2).This pattern of activity contrasts with that of Wang et al., who observed stronger antihepatoma activities of related 1,4-benzoquinone compounds compared to 1,4-dihydroxybenzene derivatives. 24hiral natural products are usually produced in nature in an optically pure form.Only occasionally are both enantiomers formed. 25As an exception, many chiral monoterpenes in plants are produced in both enantiomeric forms, often by the same species.These enantiomeric monoterpenes display unique  biological activities, oftentimes with each enantiomer exhibiting distinct biological properties.However, this phenomenon is rare among fungal terpenoids.In the case of the enantiomeric clavilactones, the opposite configuration is not generated by the terpene cyclase itself but in the course of subsequent oxidation reactions.In the proposed biosynthesis (Scheme 1), the achiral intermediate wigandolis is formed via the cyclization of geranylhydroquinone.Further oxidations, presumably catalyzed by P450 oxidases, lead to arnebinol A (which is known to coexist with clavilactone A in the plant Arnebia euchroma 25 ) and to other members of the clavilactone family.
In the case of ent-clavilactones 1 and 2, these oxidation reactions take place from the opposite direction and require three individual, antipodal steps.It will be interesting to discover how the different action of P450 enzymes is generating the different enantiomers of the clavilactones.

■ EXPERIMENTAL SECTION
General Experimental Procedures.Optical rotations were measured using an Anton Paar MCP-150 polarimeter with a 100 mm path length and sodium D line at 589 nm.UV spectra were measured on a Shimadzu UV/vis 2450 spectrophotometer using methanol (Uvasol, Merck) as a solvent.ECD spectra were measured with a J-815 spectropolarimeter (Jasco) by using methanol as a solvent.The spectral data are combined in Figure 2).1D and 2D NMR spectra were measured on a Bruker 700 MHz Avance III spectrometer equipped with a 5 mm TCI cryoprobe and a Bruker Avance III 500 spectrometer.NMR data were referenced to residual solvent peaks (δ H 7.27 ppm, δ C 77.0 ppm for CDCl 3 ; δ H 2.50 ppm, δ C 39.51 ppm for DMSO-d 6 ).
HPLC-DAD/MS data were measured using an amaZon speed ETD (electron transfer dissociation) ion trap mass spectrometer (Bruker Daltonics) and were measured in positive and negative ion modes simultaneously.The HPLC system was run with a C18 Acquity UPLC BEH (Waters) column with mobile phases of water (H 2 O, solvent A) and acetonitrile (MeCN, solvent B) each supplemented with 0.1% formic acid (FA).The following gradient conditions were used: 5% B for 0.5 min, increasing to 100% B in 20 min, hold at 100% B for 10 min, with a flow rate of 0.6 mL/min, and UV/vis detection at 200−600 nm.
HRESIMS data were recorded on a MaXis ESI-TOF (electrospray ionization-time-of-flight) mass spectrometer (Bruker GmbH) coupled to an Agilent 1260 series HPLC-UV system and equipped with a C18 X-Bridge, 100 × 2.1 mm, 3. Fungal Material.In May 2017, Resupinatus sp. was collected from an unnamed decaying dicotyledon twig in Dong Yai Community Forest, Amnat Charoen Province, located in the northeastern part of Thailand.Collections were made on behalf of the Plant Genetics Conservation Project under the Royal Initiative of Her Royal Highness Maha Chakri Sirindhorn (RSPG).The voucher specimen was deposited in the BIOTEC Bangkok Herbarium & Fungarium with the designation BBH42540.The fungal strain has been officially registered for preservation at both the National Biobank of Thailand (NBT) under the designation NBTM1549 and the BIOTEC Culture Collection under the accession number BCC84615.The genetic sequences have been submitted to the accessible GenBank under accession number OL672739.
The mycelium was separated from the supernatant with a paper filter, and both the mycelium and supernatant were extracted separately.The mycelium was covered with acetone and sonicated in an ultrasonic bath at 40 °C for 30 min, then filtered and sonicated again.The two acetone extracts were combined and evaporated in vacuo at 40 °C.The remaining aqueous residue was extracted twice with one volume of ethyl acetate each time.The supernatant was also   The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jnatprod.3c00174.

Figure 2 .
Figure 2. Experimental ECD spectra of 1 and 2; calculated ECD spectra of clavilactone J and ent-clavilactone J.

Table 1 .
Minimum Inhibitory Concentration (MIC, μg/mL) of 1 and 2 against Several Bacterial and Fungal Strains a

Table 2 .
Cytotoxicity of 1 and 2 against Mammalian Cell Lines (IC 50 in μM) a ACC: Leibniz-Institut DSMZ�German Collection of Microorganisms and Cell Cultures GmbH.b Positive control (1 mg/mL).