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Changing chemosusceptibility in the second-stage larvae of Toxocara canis by long-term incubation

Published online by Cambridge University Press:  05 June 2009

Nobuaki Akao ,
Yoshihisa Goto ,
Kaoru Kondo  and
Yoshisuke Tsuda
Nobuaki Akao
Affiliation:
Department of Parasitology, School of Medicine, Kanazawa University, 13-1 Takara-machi, Kanazawa City 920, Japan
Yoshihisa Goto
Affiliation:
Faculty of Pharmaceutical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920, Japan
Kaoru Kondo
Affiliation:
Department of Parasitology, School of Medicine, Kanazawa University, 13-1 Takara-machi, Kanazawa City 920, Japan
Yoshisuke Tsuda
Affiliation:
Faculty of Pharmaceutical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920, Japan
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Abstract

Second-stage larvae of Toxocara canis were maintained in vitro for one year. Susceptibility of the larvae to drugs was evaluated by means of minimal larvicidal concentration (MLC) and larval bursting percentage. MLCs of citral and decanoic acid were almost constant throughout all stages of incubation. However, bursting percentage markedly varied within the first 20 weeks of incubation. Therefore, while larvae are available for use in the MLC assay at any stage of incubation, those beyond the first 20 weeks after incubation should be used for the bursting assay to obtain reproducible results.

Keywords

Toxocara caniscitraldecanoic acidtannic acidchemosusceptibilityNematodasecond-stage larvae
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 2 , June 1993 , pp. 145 - 150
Copyright
Copyright © Cambridge University Press 1993

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