Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-18T05:35:12.771Z Has data issue: false hasContentIssue false

Frequent cultivation prior to planting to prevent weed competition results in an opportunity for the use of arbuscular mycorrhizal fungus inoculum

Published online by Cambridge University Press:  26 August 2011

David D. Douds*
Affiliation:
USDA-ARS Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.
Gerald Nagahashi
Affiliation:
USDA-ARS Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.
John E. Shenk
Affiliation:
Shenk's Berry Farm, 911 Disston View Dr., Lititz, PA 17543, USA.
*
*Corresponding author: david.douds@ars.usda.gov

Abstract

Inoculation with arbuscular mycorrhizal (AM) fungi is a potentially useful tool in agricultural systems with limited options regarding use of synthetic chemicals for fertility and pest control. We tested the response of Allium porrum cv. Lancelot to inoculation with AM fungi in a field high in available P (169 μg g−1 soil) that had been repeatedly cultivated to control weeds. Seedlings were inoculated during the greenhouse production period with a mixed species inoculum produced on-farm in a compost and vermiculite medium with Paspalum notatum Flugge as a nurse host. Inoculated and uninoculated seedlings were the same size at outplanting. Inoculated seedlings were over 2.5-fold greater in shoot weight and shoot P content than uninoculated seedlings at harvest. These results demonstrate the potential yield benefits from inoculation with AM fungi in situations where farm management practices may negatively impact on indigenous populations of AM fungi.

Type
Preliminary Report
Creative Commons
This is a work of the U.S. Government and is not subject to copyright protection in the United States
Copyright
Copyright © Cambridge University Press 2011. This is a work of the U.S. Government and is not subject to copyright protection in the United States.

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Smith, S.E. and Read, D.J. 2008. Mycorrhizal Symbiosis. 3rd ed.Academic Press, Amsterdam.Google Scholar
2Mozafar, A., Jansa, J., Ruh, R., Anken, T., Sanders, I., and Frossard, E. 2001. Functional diversity of AMF co-existing in agricultural soils subjected to different tillage. In Proceedings of the Third International Conference on Mycorrhizas, July 8–13, 2001, Adelaide, Australia. p. 132.Google Scholar
3Jakobsen, I. 1995. Transport of phosphorus and carbon in VA mycorrhizas. In Varma, A. and Hock, B. (eds). Mycorrhiza. Springer-Verlag, Berlin, Heidelberg. p. 297324.Google Scholar
4Bagyaraj, D.J. 1992. Vesicular-arbuscular mycorrhiza: Application in agriculture. Methods in Microbiology 24:359373.Google Scholar
5Miller, M.H., McGonigle, T.P., and Addy, H.D. 1995. Functional ecology of vesicular-arbuscular mycorrhizas as influenced by phosphate fertilization and tillage in an agricultural ecosystem. Critical Reviews in Biotechnology 15:241255.CrossRefGoogle Scholar
6Galvez, L., Douds, D.D., Wagoner, P., Longnecker, L.R., Drinkwater, L.E., and Janke, R.R. 1995. An overwintering cover crop increases inoculum of VAM fungi in agricultural soil. American Journal of Alternative Agriculture 10:152156.Google Scholar
7Sorensen, J.N., Larsen, J., and Jakobsen, I. 2005. Mycorrhiza formation and nutrient concentration in leeks (Allium porrum) in relation to previous crop and cover crop management in high P soils. Plant and Soil 273:101114.CrossRefGoogle Scholar
8Johnson, N.C., Copeland, J.P., Crookston, R.K., and Pfleger, F.L. 1992. Mycorrhizae: An explanation for yield decline in continuous corn and soybean. Agronomy Journal 84:387390.Google Scholar
9Sieverding, E. 1991. Vesicular-Arbuscular Mycorrhiza Management in Tropical Agrosystems. Duetsche Gesellschaft für Technische Zusammenarbeit (GTZ) GnbH, Eschborn.Google Scholar
10Gaur, A., Adholeya, A., and Mukerji, K.G. 2000. On-farm production of VAM inoculum and vegetable crops in marginal soil amended with organic matter. Tropical Agriculture 77:2126.Google Scholar
11Douds, D.D., Nagahashi, G., Pfeffer, P.E., Kayser, W.M., and Reider, C. 2005. On-farm production and utilization of mycorrhizal fungus inoculum. Canadian Journal of Plant Science 85:1521.Google Scholar
12Johnson, W.C. and Mullinix, B.G. 1995. Weed management in peanut using stale seedbed techniques. Weed Science 43:293297.Google Scholar
13Boyd, N., Brennan, E.B., and Fennimore, S.A. 2006. Stale seedbed techniques of organic vegetable production. Weed Technology 20:10521057.CrossRefGoogle Scholar
14Antunes, P.M., Koch, A.M., Dunfield, K.E., Hart, M.M., Downing, A., Rillig, M.C., and Klironomos, J.N. 2009. Influence of commercial inoculation with Glomus intraradices on the structure and functioning of an AM fungal community from an agricultural site. Plant and Soil 317:257266.CrossRefGoogle Scholar
15Evans, D.G. and Miller, M.H. 1990. The role of the external mycelial network in the effect of soil disturbance upon vesicular-arbuscular mycorrhizal colonization of maize. New Phytologist 114:6572.Google Scholar
16Fairchild, G.L. and Miller, M.H. 1990. Vesicular-arbuscular mycorrhizas and the soil disturbance-induced reduction of nutrient absorption in maize. III. Influence of phosphorus amendments to soil. New Phytologist 114:641650.Google Scholar
17Douds, D.D., Nagahashi, G., Pfeffer, P.E., Reider, C., and Keyser, W.M. 2006. On-farm production of AM fungus inoculum in mixtures of compost and vermiculite. Bioresource Technology 97:809818.Google Scholar
18Alexander, M. 1965. Most-probable-number method for microbial populations. In Black, C.A., Evans, D.D., Ensminger, L.E., White, J.L., and Clark, F.E. (eds). Methods of Soil Analysis, Part 2 Chemical and Microbiological Properties. American Society of Agronomy, Madison, WI, p. 14671472.Google Scholar
19Hoagland, D.R. and Arnon, D.I. 1938. The Water-Culture Method for Growing Plants without Soil. University of California College of Agriculture, Agriculture Experiment Station Circular 347, Berkeley, CA.Google Scholar
20Murphy, J. and Riley, J.P. 1962. A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica Acta 27:3136.CrossRefGoogle Scholar
21Newman, E.I. 1966. A method of estimating the total length of root in a sample. Journal of Applied Ecology 3:139145.Google Scholar
22Phillips, J.M. and Hayman, D.S. 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society 55:158160.Google Scholar
23Menge, J.A., Steirle, D., Bagyaraj, D.J., Johnson, E.L.V., and Leonard, R.T. 1978. Phosphorus concentrations in plants responsible for inhibition of mycorrhizal infection. New Phytologist 80:575578.CrossRefGoogle Scholar
24Amijee, F., Tinker, P.B., and Stribley, D.B. 1989. The development of endomycorrhizal root systems. VII. A detailed study of effects of soil phosphorus on colonization. New Phytologist 111:435446.CrossRefGoogle ScholarPubMed
25Thingstrup, I., Rubaek, G., Sibbeson, E., and Jakobsen, I. 1998. Flax (Linum usitatissimum L.) depends on arbuscular mycorrhizal fungi for growth and P uptake at intermediate but not at high soil P levels in the field. Plant and Soil 203:3746.CrossRefGoogle Scholar
26Hamel, C., Dalpé, Y., Furlan, V., and Parent, S. 1997. Indigenous populations of arbuscular mycorrhizal fungi and soil aggregate stability are major determinants of leek (Allium porrum L.) response to inoculation with Glomus intraradices Schenck and Smith or Glomus versiforme (Karsten) Berch. Mycorrhiza 7:187196.Google Scholar
27Thompson, J.P. 1987. Decline of vesicular-arbuscular mycorrhizae in long fallow disorder of field crops and its expression in phosphorus deficiency of sunflower. Journal of Agricultural Research 38:847867.Google Scholar
28Schreiner, R.P. and Koide, R.T. 1993. Mustards, mustard oils, and mycorrhizas. New Phytologist 123:107113.Google Scholar
29Johnson, N.C. 1993. Can fertilization select less mutualistic mycorrhizae? Ecological Applications 3:749757.CrossRefGoogle ScholarPubMed
30Koide, R.T., Landherr, L.L., Besmer, Y.L., Detweiler, J.M., and Holcomb, E.J. 1999 Strategies for mycorrhizal inoculation of six annual bedding plant species. HortScience 34:12171220.Google Scholar
31Douds, D.D., Nagahashi, G., Shenk, J.E., and Demchak, K. 2008. Inoculation of strawberries with AM fungi produced on-farm increased yield. Biological Agriculture and Horticulture 26:209219.Google Scholar
32Sorensen, J.N., Larsen, J., and Jakobsen, I. 2008. Pre-inoculation with arbuscular mycorrhizal fungi increases early nutrient concentration and growth of field-grown leeks under high productivity conditions. Plant and Soil 307:135147.Google Scholar