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Propensity in low-grade oocytes for delayed germinal vesicle breakdown compromises the developmental ability of sub-optimal grade Bubalus bubalis oocytes

Published online by Cambridge University Press:  05 October 2018

Manish Kumar ,
Mona Faraji ,
Parul Sarwalia ,
Sandeep Kumar ,
Moloya Gohain ,
Sachinandan De ,
Rakesh Kumar Open the ORCID record for Rakesh Kumar [Opens in a new window]  and
T K Datta
Manish Kumar
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia
Mona Faraji
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia
Parul Sarwalia
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia
Sandeep Kumar
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia
Moloya Gohain
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia Institute of Plant and Microbial Biology (IPMB) Sec 128, Academia Road, Nangang District, Taipei City – 11529Taiwan
Sachinandan De
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia
Rakesh Kumar
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia
T K Datta*
Affiliation:
Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001HaryanaIndia
*
*Author for correspondence: T K Datta. Animal Genomics Laboratory, Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001 Haryana India. Tel: +91 184 2259506. Fax: +91 184 2250042. E-mail: tirthadatta@gmail.com
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Summary

Maturing oocytes have diverse developmental potential and good quality oocytes exhibit a better ability to attain physiological milestones in a time-dependent manner. This situation necessitates the confirmation of oocyte developmental status more precisely under an in vitro embryo production (IVEP) regime. The aim of this study was to explain timely events in germinal vesicle breakdown (GVBD), an important milestone of oocyte nuclear maturation, to delineate the developmental capacity of Bubalus bubalis oocytes. In addition, the expression profile of genes responsible for GVBD was assessed in order to understand the molecular context responsible for GVBD. The chronology of GVBD events at different time intervals during in vitro maturation (IVM) suggests that the rate at which oocytes undergo GVBD was strikingly different in the brilliant cresyl blue (BCB)+ and BCB− groups. The expression of AKT and CDC25B genes for BCB+ oocytes was maximum at 8 h of IVM, and CCNB (cyclin B) peaked at around 10 h, which suggested that GVBD was finished after 10 h in BCB+ oocytes, whereas the expression of AKT and CDC25B was found to peak at around 12–14 h of IVM. This difference consequently delays the GVBD event by 2–4 h in BCB− oocytes. Poor abundance of gene transcripts was mainly implicated in delay and lower rate of GVBD in BCB− oocytes which in turn strongly affected the translational ability of oocytes to blastocysts. The findings of this study support the idea that there is a propensity in sub-optimal grade oocytes for delayed GVBD that compromises the developmental ability of low grade buffalo oocytes. The study highlights the very small, but importantly vital and separate, time window of the GVBD event during which the competence levels of buffalo oocytes are altered along with their translational ability to develop into the prospective embryos.

Keywords

Aceto-orcein stainingBrilliant cresyl blueGVBDOocyte competence
Type
Research Article
Information
Zygote , Volume 26 , Issue 5 , October 2018 , pp. 359 - 365
Copyright
© Cambridge University Press 2018 

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Footnotes

*

These authors contributed equally to this work.

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