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Inhibition of sperm-egg fusion in the hamster and mouse by carbohydrates

Published online by Cambridge University Press:  26 September 2008

Ruben H. Ponce ,
Umbert A. Urch  and
Ryuzo Yanagimachi
Ruben H. Ponce
Affiliation:
University of Hawaii shool of Medicine, Hawaii, and University of california, Davis, california, USA
Umbert A. Urch*
Affiliation:
University of Hawaii shool of Medicine, Hawaii, and University of california, Davis, california, USA
Ryuzo Yanagimachi
Affiliation:
University of Hawaii shool of Medicine, Hawaii, and University of california, Davis, california, USA
*
U.A.Urch, Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA. 916-752-3085.
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Summary

After spermatozoa bind to and penetrate the extracellular matrix of the egg, the zona pellucida, they adhere to and fuse with the plasma membrane of the egg. Since sperm–egg fusion may involve membrane glycoproteins and/or carbohydrate binding proteins, we sought to test this hypothesis by challenging sperm–egg fusion in hamster and in mouse with added carbohydrates. In this study, a number of carbohydrate and glycoconjugates were examined for their ability to inhibit sperm–eggfusion. In the hamster, D(+)-glucosamine, D(+)-galactosamine, albumin-bovine-glucosamide and-galactosamide, fucoidan and dextran sulphate inhibited the fusion of spermatozoa with zona-free eggs. The same effects were seen in the mouse, except for the toxic effects of D(+)-galactosamine. These facts suggest a role of carbohydrate binding proteins or glycoproteins in the fertilisation process at the level of binding to and fusing with the oolemma.

Keywords

EggFertilisationMembrane fusionSpermatozoa
Type
Article
Information
Zygote , Volume 2 , Issue 3 , August 1994 , pp. 253 - 262
Copyright
Copyright © Cambridge University Press 1994

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