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Application of pulsed-field gel electrophoresis to the epidemiological characterization of Staphylococcus intermedius implicated in a food-related outbreak

Published online by Cambridge University Press:  15 May 2009

F. M. Khambaty
Affiliation:
Division of Microbiological Studies, U.S. Food and Drug Administration, Washington, D.C. 20204, USA
R. W. Bennett
Affiliation:
Division of Microbiological Studies, U.S. Food and Drug Administration, Washington, D.C. 20204, USA
D. B. Shah
Affiliation:
Division of Microbiological Studies, U.S. Food and Drug Administration, Washington, D.C. 20204, USA
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An outbreak of food intoxication involving over 265 cases in western United States occurred in October 1991. Staphylococcus intermedius was implicated as the aetiologic agent. Representative outbreak isolates (five clinical and ten from foods) produced type A enterotoxin. DNA fragments generated by four restriction endonucleases and analysed by pulsed-field gel electrophoresis (PFGE) provided definitive evidence that all isolates from nine different counties in California and Nevada were derived from a single strain. The PFGE pattern of these outbreak isolates was distinct from those of a heterogeneous collection of seven S. intermedius strains of veterinary origin and five unrelated S. aureus laboratory strains. The data show a significant PFGE pattern heterogeneity not only among members of different Staphylococcus species but also within members of the same species and even the same enterotoxin type. The results indicate that PFGE is a valuable strain-specific discriminator for the epidemiological characterization of S. intermedius. To our knowledge, this represents the first documented foodborne outbreak caused by S. intermedius. These findings suggest that the presence of S. intermedius and other species such as S. hyicus in food should be reason for concern.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Ølsvik, O, Fossum, K. Berdal, BP. Staphylococcal enterotoxin A, B, and C produced by coagulase-negative strains within the family Micrococcaceae. Path Microbiol Immunol Scand Sect B. 1982; 90: 441–4.Google Scholar
2.Raus, J, Love, DN. Characterization of coagulase-positive Staphylococcus intermedius and Staphylococcus aureus isolated from veterinary clinical specimens. J Clin Microbiol 1983; 18: 789–92.CrossRefGoogle ScholarPubMed
3.Talan, DA, Staatz, D, Staatz, A, Goldstein, EJ, Singer, K, Overturf, GD. Staphylococcus intermedius in canine gingiva and canine-inflicted human wound infections: laboratory characterization of a newly recognized zoonotic pathogen. J Clin Microbiol 1989; 27: 7881.CrossRefGoogle ScholarPubMed
4.Talan, DA, Staatz, D, Staatz, A, Overturf, GD. Frequency of Staphylococcus intermedius as human nasopharyngeal flora. J Clin Microbiol 1989; 27: 2393.Google Scholar
5.Almazan, J, de la Fuente, R, Gomez-Lucia, E. Suarez, G. Enterotoxin production by strains of Staphylococcus intermedius and Staphylococcus aureus isolated from dog infections. Zentralbl Bakteriol Mikrobiol Hyg A 1987; 264: 2932.Google Scholar
6.Hirooka, EY, Muller, EE. Isolamento, caracterizacad e isolados de productos carneos comercializados em Londrina-PR. Cien Technol Aliment 1982; 2: 123–33.Google Scholar
7.Hajek, V. Staphylococcus intermedius, a new species isolated from animals. Int J Syst Bacteriol 1976; 26: 401–8.CrossRefGoogle Scholar
8.Hajek, V, Marsalek, E. Evaluation of classifactory criteria for staphylococci. In: Jeljaszewwicz, J, ed., Staphylococci and staphylococcal diseases: Proceedings of the 3rd international symposium on staphylococci and staphylococcal infections. Stuttgart and New York; Gustav Finscher Verlag Publishers, 1976.Google Scholar
9.Kloos, WE, Schleifer, KH. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol 1975; 1: 82–8.Google Scholar
10.Fukada, S, Tokuno, H. Ogawa, H et al. , Enterotoxigenicity of Staphylococcus intermedius strains isolated from dogs. Zentralbl Bakteriol Hyg A 1984; 258: 360–7.Google Scholar
11.Hirooka, EY. Muller, EE, Freitas, JC, Vincente, E, Yoshimoto, Y, Bergdoll, MS. Enterotoxicity of Staphylococcus intermedius of canine origin. Int J Food Microbiol 1988; 7: 185–91.Google Scholar
12. Anonymous. Outbreak that didn't seem right spread like butter. Food Prot Rep 1992; 8: 56.Google Scholar
13.Poddar, SK. A modified method of genomic DNA preparation in agarose insert for pulse field gel electrophoresis. Electrophoresis 1991; 12: 674–5.CrossRefGoogle ScholarPubMed
14.Bennett, RW. Staphylococcal enterotoxins. In: U.S. FDA Bacteriological Analytical Manual, 7th ed.AOAC International, Arlington, VA, 1992: 167–90.Google Scholar
15.Goering, RV, Duensing, TD. Rapid field inversion gel electrophoresis in combination with a rRNA gene probe in the epidemiological evaluation of staphylococci. J Clin Microbiol 1990; 28: 426–9.CrossRefGoogle ScholarPubMed
16.Poddar, SK, McClelland, M. Restriction fragment fingerprint and genome sizes of Staphylococcus species using pulsed-field gel electrophoresis and infrequent cleaving enzymes. DNA Cell Biol 1991; 10: 663–9.CrossRefGoogle ScholarPubMed
17.Adesiyun, AA. Production of enterotoxin(s) by Staphylococcus hyicus. Vet Microbiol 1984; 9: 487–95.Google Scholar