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Electrophoretic characterisation of the outer membrane proteins of Yersinia pestis isolated in north-east Brazil

Published online by Cambridge University Press:  15 May 2009

F. G. C. Abath ,
A. M. P. Almeida  and
L. C. S. Ferreira
F. G. C. Abath
Affiliation:
Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE-59.739Brazil
A. M. P. Almeida
Affiliation:
Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE-59.739Brazil
L. C. S. Ferreira*
Affiliation:
Laboratório de Imunopatologia Keizo Asami and Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, PE-59.739Brazil
*
*Dr L. C. S. Ferreira. Laboratório de Imunopatologia Keizo Asami, Cidade Universitária, Recife, PE-50. 739. Brazil
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The outer membrane proteins of 38 Yersinia pestis isolates from all known plague foci of north-east Brazil were analysed by SDS-PAGE. Approximately 20 bands were consistently found in all strains analysed and 11 were selected for comparative studies. Although qualitative differences among the electrophoretic profiles of outer membrane proteins of wild Y. pestis isolates were not observed, quantitative alterations were clearly noted for most of these proteins. No particular quantitative alteration of the electrophoretic profile of outer membrane proteins could be associated with the period of isolation and geographic origin of the isolates. The 64 kDa outer membrane protein was significantly expressed in higher amounts among Y. pestis strains isolated from a recent plague outbreak. The possible use of electrophoretic profiles of outer membrane proteins of wild Y. pestis isolates as a tool for epidemiological studies and for the analysis of virulence determinants is discussed.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 595 - 602
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Mollaret, HH, Thal, E., Genus, XIYersinia Van Loghem 1944. In: Buchanan, RE, Gibbons, NE, eds. Bergey's manual of determinative bacteriology, 8th. ed.Baltimore: Williams & Wilkins, 1974; 330–2.Google Scholar
2.Devignat, R.Repartition geographique des trois varietés de Pasteurella pestis. Schweiz Z Allgemeine Pathol Bakt 1953; 16: 509–15.Google ScholarPubMed
3.Hudson, BW, Quan, TJ, Bailey, RE.Electrophoretic studies of the geographic distribution of Yersinia pestis protein variants. Int J Syst Bact 1976; 26: 1–16.CrossRefGoogle Scholar
4.Mello, DA, Vasconcelos, LM.Classification of varieties of Pasteurella pestis strains isolated in the interior of the state of Pernambuco, Brasil. Rev Soc Bras Med Trop 1969; 3: 269–271.CrossRefGoogle Scholar
5.Karimi, Y, Almeida, CR, Almeida, AMP, Keyvanfar, A, Bourdin, M.Particularités des souches de Yersinia pestis isolés dans Ie nord-est du Brésil. Ann Inst Pasteur Microbiol 1971; 125A: 213–6.Google Scholar
6.Hudson, BW, Quan, TJ, Sites, VR, Marshall, JD.An electrophoretic and bacteriologic study of Yersinia pestis isolates from Central Java, Asia and the Western hemisphere. Am J Trop Med Hyg 1973; 22: 642–53.CrossRefGoogle ScholarPubMed
7.Hudson, BW, Quan, TJ.Electrophoretic studies of the Yersiniae. Am J Trop Med Hyg 1975; 24: 968–73.CrossRefGoogle ScholarPubMed
8.Straley, SC, Brubaker, RR.Localization in Yersinia pestis of peptides associated with virulence. Infect Imm 1982; 36: 129–35.CrossRefGoogle ScholarPubMed
9.Bolin, I, Forsberg, A, Norlander, L, Skurnik, M, Wolf-Watz, H.Identification and mapping of the temperature-inducible, plasmid-encoded proteins of Yersinia spp. Infect Imm 1988; 56: 343–8.CrossRefGoogle ScholarPubMed
10.Laemmli, UK.Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–5.CrossRefGoogle ScholarPubMed
11.Morrissey, JM.Silver stain for proteins in polyacrilamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 1981; 117: 307–10.CrossRefGoogle Scholar
12.Lowry, OH, Rosebrough, NJ, Farr, AL, Randal, RJ.Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265–75.CrossRefGoogle ScholarPubMed
13.Baltazard, M.Viagem de estudo ao Brasil para a organização de um projeto de pesquisas sobre a peste. Rev Bras Malariol Doenças Trop 1968; 20: 335–67.Google Scholar
14.Almeida, AMP, Carvalho, FG, Almeida, CR.Isolamento da Yersinia pestis nos focos pestosos do nordeste do Brasil no período de 1966 a 1982. Rev Inst Med Trop São Paulo 1985; 27: 207–18.CrossRefGoogle Scholar
15.Almeida, AMP, Brasil, DB, Leal, NC, Melo, MEB, Rego, RVB, Almeida, CR.Estudos bacteriológicos e sorológicos de um surto de peste no Estado da Paraíba, Brasil. Mem Inst Oswaldo Cruz 1989; 84: 249–56.CrossRefGoogle Scholar
16.Devignat, R.Varités de l'espèce Pasteurella pestis: nouvelle hypothèse. Bull WHO 1951; 4: 247–63.Google Scholar
17.Seguin, MC, Harrison, DN, Williams, JE.A rapid and safe procedure for preparing the murine toxins of the plague bacillus (Yersinia pestis). Toxicon 1987; 25: 989–94.CrossRefGoogle ScholarPubMed