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The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic

Published online by Cambridge University Press:  20 March 2018

L. Lecová Open the ORCID record for L. Lecová [Opens in a new window] ,
F. Weisz ,
P. Tůmová ,
V. Tolarová  and
E. Nohýnková
L. Lecová*
Affiliation:
Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Studničkova 7, 128 00, Prague, Czech Republic
F. Weisz
Affiliation:
Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Studničkova 7, 128 00, Prague, Czech Republic
P. Tůmová
Affiliation:
Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Studničkova 7, 128 00, Prague, Czech Republic
V. Tolarová
Affiliation:
Regional Institute of Public Health, Sokolovská 60, 186 00, Prague, Czech Republic
E. Nohýnková
Affiliation:
Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Studničkova 7, 128 00, Prague, Czech Republic
*
Author for correspondence: Lenka Lecová, E-mail: lenka.lecova@lf1.cuni.cz
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Abstract

To date, genotyping data on giardiasis have not been available in the Czech Republic. In this study, we characterized 47 human isolates of Giardia intestinalis from symptomatic as well as asymptomatic giardiasis cases. Genomic DNA from trophozoites was tested by PCR-sequence analysis at three loci (β-giardin, glutamate dehydrogenase and triose phosphate isomerase). Sequence analysis showed assemblages A and B in 41 (87.2%) and six (12.8%) isolates, respectively. Two of the 41 assemblage A samples were genotyped as sub-assemblage AI, and 39 were genotyped as sub-assemblage AII. Four previously identified multilocus genotypes (MLGs: AI-1, AII-1, AII-4 and AII-9) and six likely novel variations of MLGs were found. In agreement with previous studies, sequences from assemblage B isolates were characterized by a large genetic variability and by the presence of heterogeneous positions, which prevent the definition of MLGs. This study also investigated whether there was a relationship between the assemblage and clinical data (including drug resistance). However, due to the large number of genotypes and the relatively small number of samples, no significant associations with the clinical data were found.

Keywords

Assemblageclinical resistancegenotypeGiardia intestinalismultilocus genotypingsubtypes
Type
Research Article
Information
Parasitology , Volume 145 , Issue 12 , October 2018 , pp. 1577 - 1587
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Copyright © Cambridge University Press 2018 

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