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Changes in hepatic lipids of mice infected with cysticerci of Taenia crassiceps

Published online by Cambridge University Press:  12 April 2024

I. Corbin ,
M. Novak  and
B.J. Blackburn
I. Corbin
Affiliation:
Department of Biology and, University of Winnipeg, Winnipeg, Manitoba, Canada, R3B 2E9
M. Novak*
Affiliation:
Department of Biology and, University of Winnipeg, Winnipeg, Manitoba, Canada, R3B 2E9
B.J. Blackburn
Affiliation:
Department of Chemistry, University of Winnipeg, Winnipeg, Manitoba, Canada, R3B 2E9
*
*Author for correspondence. Fax: (204) 774 4134 E-mail: mnovak@uwinnipeg.ca
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Abstract

Nuclear magnetic resonance (NMR) spectroscopy was employed to investigate the effect of infection with Taenia crassiceps cysticerci on the lipid profile of mouse liver. Chloroform/methanol extracts of livers from infected mice showed lower concentrations of phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, total glycerophospholipid, triacylglycerol, total fatty acid (FA) and all measured FA components than those from controls. Furthermore, the ratios obtained on dividing concentrations of the FA components by that of total FA demonstrate that the concentration decreases caused by infection are less for polyunsaturated fatty acids (FAs) than for other FAs. Extracts of T. crassiceps displayed a similar lipid profile to that of host liver but contained a lower lipid content and a shorter average FA chain length.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 3 , September 2000 , pp. 209 - 214
Copyright
Copyright © Cambridge University Press 2000

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References

Adosraku, R.K., Anderson, M.M., Anderson, G.J., Choi, C., Yardely, V., Phillipson, J.D. & Gibbons, W.A. (1993) Proton NMR lipid profile of Leishmania donovani promastigotes. Molecular and Biochemical Parasitology 62, 251–262.CrossRefGoogle ScholarPubMed
Adosraku, R.K., Choi, G.T.Y., Constantinou-Kokotos, V., Anderson, M.M. & Gibbons, W.A. (1994) NMR lipid profiles of cells, tissues and body fluids: proton NMR analysis of human erythrocyte lipids. Journal of Lipid Research 35, 1925–1931.CrossRefGoogle ScholarPubMed
Ansell, G.B. & Spanner, S. (1982) Phosphatidylserine, phosphatidylethanolamine and phosphatidylcholine. pp. 1–49 in Hawthorne, J.N. & Ansell, G.B. (Eds) Phospholipids. New York, Elsevier Biomedical Press.Google Scholar
Barrett, J. (1983) Lipid metabolism. pp. 391–420 in Arme, C. & Pappas, P.W. (Eds) Biology of Eucestoda. London, Academic Press.Google Scholar
Belley, A. & Chadee, K. (1995) Eicosanoid production by parasites: from pathogenesis to immunomodulation?. Parasitology Today 11, 327–334.CrossRefGoogle ScholarPubMed
Casu, M., Anderson, G.J., Choi, G. & Gibbons, W.A. (1991) NMR lipid profiles of cells, tissues and body fluids — 1D and 2D proton NMR of lipids from rat liver. Magnetic Resonance in Chemistry 29, 594–602.CrossRefGoogle Scholar
Chen, Z.Y. & Cunnane, S.C. (1992) Preferential retention of linoleic acid-enriched triacylglycerols in liver and serum during fasting. American Journal of Physiology 263, R233–R239 Google ScholarPubMed
Chernin, J. & McLaren, D. (1983) The pathology induced in laboratory rats by metacestodes of Taenia crassiceps and Mesocestoides corti (Cestoda). Parasitology 87, 279–287.CrossRefGoogle ScholarPubMed
Choi, G.T.Y., Casu, M. & Gibbons, W.A. (1993) NMR lipid profiles of cells, tissues and body fluids: neutral, non-acidic and acidic phospholipid analysis of Bond Elut chromatographic fractions. Biochemistry Journal 290, 717–721.CrossRefGoogle ScholarPubMed
Christie, W.W. (1982) Lipid analysis: isolation, separation, identification and structural analysis of lipids. Oxford, Pergamon Press.Google Scholar
Corbin, I., Blackburn, B.J., Wolowiec, T. & Novak, M. (1996) Metabolite profile of the liver of mice infected with cysticerci of Taenia crassiceps . Parasitology Research 82, 273–275.CrossRefGoogle Scholar
Corbin, I., Blackburn, B.J. & Novak, M. (1999) Metabolism of [3-13C]alanine in liver of mice infected with cysticerci of Taenia crassiceps . Canadian Journal of Zoology.CrossRefGoogle Scholar
Crompton, D.W.T., Arnold, S.E., Walters, D.E. & Whitfield, P.J. (1985) Food intake and body weight changes in mice infected with metacestodes of Taenia crassiceps . Parasitology 90, 449–456.CrossRefGoogle ScholarPubMed
Cunnane, S.C. (1988) Differential utilization of long chain fatty acid during triacylglycerol depletion. II. Rat liver after starvation. Lipids 23, 372–374.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Sloane-Stanley, G.H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Frayha, G.J. & Smyth, J.D. (1983) Lipid metabolism in parasitic helminths. Advances in Parasitology 22, 309–387.CrossRefGoogle ScholarPubMed
Freeman, R.S. (1962) Studies of the biology of Taenia crassiceps . Canadian Journal of Zoology 40, 96–990.Google Scholar
Freeman, R.S. (1964) Studies on responses of intermediate host to infection with Taenia crassiceps . Canadian Journal of Zoology 42, 367–385.CrossRefGoogle Scholar
Gavino, G.R. & Gavino, V.C. (1991) Rat outer mitochondrial carnitine palmitoyltransferase activity towards long chain polyunsaturated fatty acids and their CoA esters. Lipids 26, 266–270.CrossRefGoogle ScholarPubMed
Gemmell, M.A., Matyas, Z., Pawlowski, Z., Soulsby, E.J.L., Larralde, C., Nelson, G.S. & Rosicky, B. (1983) Guidelines for surveillance prevention and control of taeniasis/cysticercosis. WHO document VPH/83.49. Geneva, Switzerland.Google Scholar
Goodridge, A.G., Donald, W.B., Wilson, S.B. & Goldman, M.T. (1986) Regulation of genes for enzymes involved in fatty acid synthesis. Annals of the New York Academy of Sciences 478, 46–62.CrossRefGoogle ScholarPubMed
Hustead, S.T. & Williams, J.F. (1977) Permeability studies on taeniid metacestodes: uptake of protein by larval stages of Taenia taeniaeformis, Taenia crassiceps and Echinococcus granulosus . Journal of Parasitology 63, 314–321.CrossRefGoogle ScholarPubMed
Katz, M. (1986) Metabolism of lipids. pp. 237–252 in Thurman, R.G., Kuffman, F.C. & Jungermann, K. (Eds) Regulation of hepatic metabolism: intra- and intercellular compartmentation. New York, Plenum Press.CrossRefGoogle Scholar
Keppler, D. (1994) Leucotrienes and other eicosanoids in liver pathology. pp. 1015–1029 in Arias, I.M., Boyer, J.L., Fausto, N., Jakoby, W.B., Schachetr, D.A. & Shafritz, D.A. (Eds) Liver biology and pathobiology. New York, Raven Press.Google Scholar
Larralde, C., Sotelo, J., Montoya, M., Palencia, G., Padilla, A., Govezensky, T., Diaz, M.L. & Sciutto, E. (1990) Immunodiagnosis of human cysticercosis in cerebrospinal fluid: antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium . Archives of Pathology and Laboratory Medicine 114, 926–928.Google ScholarPubMed
Marinetti, G.V. (1990) Disorders of lipid metabolism. New York, Plenum Press.CrossRefGoogle Scholar
Mathews, C.K. & van Holde, K.E. (1990) Biochemistry. California, Benjamin/Cummings Publ. Co.Google Scholar
Mills, G.L., Taylor, D.C. & Williams, J.F. (1981) The lipid composition of the helminth parasite Taenia crassiceps . Comparative Biochemistry and Physiology 69, 553–557.Google Scholar
Pollesello, P., Masutti, F., Croce, L.S., Toffanin, R., Eriksson, O., Paoletti, S., Hockerstedt, K. & Tiribelli, C. (1993) H NMR spectroscopic studies of lipid extracts from human fatty liver. Biochemical and Biophysical Research Communications 192, 1217–1222.CrossRefGoogle Scholar
Sciutto, E., Fragoso, G., Trueba, L., Lemus, D., Montoya, R.M., Diaz, M.L., Govezensky, T., Lomeli, C., Tapia, G. & Larralde, C. (1990) Cysticercosis vaccine: cross protecting immunity with T. solium antigens against experimental murine T. crassiceps cysticercosis. Parasite Immunology 12, 687–696.CrossRefGoogle Scholar
Sciutto, E., Fragoso, G., Baca, M., Dela Cruz, V., Lemus, L. & Lamoyi, E. (1995) Depressed T-cell proliferation associated with susceptibility to experimental Taenia crassiceps infection. Infection and Immunology 63, 2277–2281.CrossRefGoogle ScholarPubMed
Seifter, S. & Englard, S. (1994) Energy metabolism. pp. 323–364 in Arias, I.M., Boyer, J.L., Fausto, N., Jakoby, W.B., Schachter, D.A. & Shafritz, D.A. (Eds) Liver biology and pathobiology. New York, Raven Press.Google Scholar
Shea, M., Maberley, A.L., Walters, J. & Freeman, R. (1972) Tapeworms turn up in a new place, the eye. Journal of the American Medical Association 222, 891–1192.Google Scholar
Shiau, Y.F. (1987) Lipid digestion and absorption. pp. 1527–1556 in Johnson, L.R. (Ed) Physiology of the gastrointestinal tract. New York, Raven Press.Google Scholar
Smith, E.L., Hill, R.L., Lehman, I.R., Lefkowitz, R.J., Handler, P. & White, A. (1983) Principles of biochemistry: mammalian biochemistry. New York, McGraw-Hill Co.Google Scholar
Smith, W.L., Borgeat, P. & Fitzpatrick, F.A. (1991) The eicosanoids: lipoxygenase and epoxygenase pathways. pp. 297–325 in Vance, D.E. & Vance, J. (Eds) Biochemistry of lipids, lipoproteins and membranes. Amsterdam, Elsevier. Smyth, J.D. & McManus, D.P. (1989) The physiology and biochemistry of cestodes. New York, Cambridge University Press.Google Scholar
Sparling, M.L., Zidovetzki, R., Muller, L. & Sunney, I.C. (1989) Analysis of membrance lipids by 500 Mhz 1H NMR. Analytical Biochemistry 178, 67–76.CrossRefGoogle Scholar
Sugden, M.C., Howard, R.M., Munday, M.R. & Holness, M.J. (1993) Mechanisms involved in the coordinate regulation of strategic enzymes of glucose metabolism. Advances in Enzyme Regulation 33, 71–95.CrossRefGoogle ScholarPubMed
Sze, D.Y. & Jardetzky, O. (1990) Determination of metabolite and nucleotide concentrations in proliferating lymphocytes by 1H-NMR of acid extracts. Biochimica et Biophysica Acta 1054, 181–197.CrossRefGoogle ScholarPubMed
Vance, D.E. (1991) Phospholipid metabolism and cell signalling in eucaryotes. pp. 205–240 in, Vance, D.E. & Vance, J. (Eds) Biochemistry of lipids, lipoproteins and membranes. Amsterdam, Elsevier Science Publishers.Google Scholar
Villa, O.F. & Kuhn, R.E. (1996) Mice infected with larvae of Taenia crassiceps exhibit a Th2-like immune response with concomitant anergy and downregulation of Th1-associated phenomena. Parasitology 112, 561–570.CrossRefGoogle ScholarPubMed
Williams, J.F., Engelkirk, P.G. & Lindsay, M.A. (1982) Mechanisms of immunity in rodent cysticercosis. pp. 621–632 in Flisser, A., Willms, K., Laclette, J.P., Larralde, C., Ridaura, C., Beltran, F. & Vogt, M.W. (Eds) Cysticercosis: present state of knowledge and perspectives. New York, Academic Press.Google Scholar