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Biochemical characterization and genetic mapping of purine genes in Micrococcus luteus

Published online by Cambridge University Press:  14 April 2009

Nirupama Mohapatra  and
Wesley E. Kloos
Nirupama Mohapatra
Affiliation:
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27607
Wesley E. Kloos
Affiliation:
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27607
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Purine auxotrophs of Micrococcus luteus were induced by N-methyl-N′-nitro-N-nitrosoguanidine. They were biochemically characterized by growth stimulation with CO2-enriched air and purine intermediates and by the Bratton-Marshall test that is used to detect accumulated diazotizable amines. The mutants were divided into four classes: pur (EC), purH, purJ, and Pur−. The pur(EC) class was further subdivided into purE and purC by results of crossing all the pur(EC) mutants with a known purE reference strain (ATCC 27141). Mutants which were not linked to the reference purE marker were considered to be purC. Genetic mapping was accomplished by using two-point reciprocal transformation crosses. The result of this study indicates that purJ mutants may be loosely linked to purE and purC, and purH mutants are not linked to purE, purC or purJ.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 2 , October 1975 , pp. 163 - 171
Copyright
Copyright © Cambridge University Press 1975

References

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