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Serotonin receptors are selectively expressed in the avian germ cells and early embryos

Published online by Cambridge University Press:  13 February 2014

Urszula Stępińska ,
Takashi Kuwana  and
Bożenna Olszańska
Urszula Stępińska
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec n/Warsaw, Magdalenka 05–552, Poland.
Takashi Kuwana
Affiliation:
International Institute of Avian Conservation Science, Abu-Dhabi, United Arab Emirates.
Bożenna Olszańska*
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec n/Warsaw, Magdalenka 05-552, Poland.
*
All correspondence to: B. Olszańska. Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec n/Warsaw, Magdalenka 05-552, Poland. Tel: +48 22 736 70 33. e-mail: B.Olszanska@ighz.pl
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Summary

The expression of nine serotonin (5-HT) receptor transcripts was studied using reverse transcription polymerase chain reaction (RT-PCR) in germ cells, cleavage and gastrulation stages of Japanese quail, and qPCR for 5-HT3 and 5-HT4 receptors in oocytes and embryos. We show the presence/absence of nine serotonin transcripts known in birds for receptors 5-HT1A, 5-HT1F, 5-HT2B, 5-HT2C, 5-HT3, 5-HT4, 5-HT5A, 5-HT6 and 5-HT7A in avian germ cells and early embryos. The absence of 5-HT3 and 5-HT5A in primordial germ cells and of 5-HT3 and 5-HT7A in sperm is characteristic. All transcripts appeared in oocytes at all stages (except for 5-HT3 and 5-HT5A transcripts) and all were present in cleaving embryos and at gastrulation, except for 5-HT3, which was permanently observed as late as in stage 4. Interestingly, 5-HT3 and 5-HT5A receptors accumulated in 3-mm and F1 oocytes but were degraded at ovulation and started to be re-transcribed in cleavage stage II embryos and beyond. The selective appearance of 5-HT receptors in germ cells and early embryos supports the hypothesis that serotonin may act as a signalling molecule at early stages of germ line and embryo differentiation via individual receptors present during different stages, when specialized communication systems are not yet developed.

Keywords

5-HT receptor transcriptsEmbryoOocytePrimordial Germ CellsSperm
Type
Research Article
Information
Zygote , Volume 23 , Issue 3 , June 2015 , pp. 394 - 405
Copyright
Copyright © Cambridge University Press 2014 

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