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The fine structure of sexual stage development and sporogony of Cryptosporidium parvum in cell-free culture

Published online by Cambridge University Press:  03 March 2016

HEBATALLA M. ALDEYARBI  and
PANAGIOTIS KARANIS
HEBATALLA M. ALDEYARBI
Affiliation:
University of Cologne, Center for Anatomy, Institute I, Joseph-Stelzmann-Street 9, 50937 Cologne, Germany Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, 41522, Egypt
PANAGIOTIS KARANIS*
Affiliation:
University of Cologne, Medical School, Cologne, Germany Thousand Talents Plan of the Chinese Government, Center for Biomedicine and Infectious Diseases, Qinghai Academy of Animal Science and Veterinary Medicine, Xining, China
*
*Corresponding author: H. Aldeyarbi, University of Cologne, Center for Anatomy, Institute I, Joseph-Stelzmann-Street 9, 50937 Cologne, Germany. Telephone/FAX number: +49-2214785202. E-mail: heba_deyarbi@daad-alumni.de
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Summary

The sexual stages and new oocysts development of Cryptosporidium parvum were investigated in a cell-free culture system using transmission electron microscopy (TEM). Sexual development was extremely rapid after inoculation of oocysts into the medium. The process began within 1/2–12 h and was completed with new oocyst formation 120 h post-inoculation. The macrogamonts were bounded by two membranes and had amylopectin granules and two distinct types of wall-forming bodies. The microgamonts had a large nucleus showing lobe projections and condensation of chromatin, giving rise to peripherally budding microgametes. The microgametes contained a large area of granular substance containing groups of microtubules surrounding the electron-dense nucleus. In some instances, the dividing microgamy was observed in cell-free cultures with no preceding merogonic process. Fertilization was observed with the bullet-shaped microgamete penetrating an immature macrogamont at 24 and 216 h. The new thin- and thick-walled oocysts had a large residuum with polysaccharide granules and sporogony noted inside these oocysts. Novel immature four-layer walled thick oocysts with irregular knob-like protrusions on the outer layer resembling the immature Eimeria oocysts were also observed. The present study confirms the gametogony and sporogony of C. parvum in cell-free culture and describes their ultra-structure for the first time.

Keywords

Host cell-free culturesexual life cyclethin-walled oocyststhick-walled oocystsimmature thick oocystsextracellular development
Type
Research Article
Information
Parasitology , Volume 143 , Issue 6 , May 2016 , pp. 749 - 761
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Copyright
Copyright © Cambridge University Press 2016 

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