Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-06-04T14:12:54.765Z Has data issue: false hasContentIssue false
  • English
  • Français

Antigenic characterization of adult Wuchereria bancrofti filarial nematodes

Published online by Cambridge University Press:  06 April 2009

T. M. Morgan ,
Inge Sutanto ,
Purnomo ,
Sukartono ,
F. Partono  and
R. M. Maizels
T. M. Morgan
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, London SW7 2BB, UK
Inge Sutanto
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
Purnomo
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
Sukartono
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
F. Partono
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
R. M. Maizels
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, London SW7 2BB, UK
Get access Rights & Permissions [Opens in a new window]

Summary

Adult Wuchereria bancrofti were recovered from infected Presbytis cristatus monkeys and radio-isotope labelled extrinsically with 125I and in vitro with [35S]methionine. 125I labelling of the surface of adult W. bancrofti permitted a comparison between the major surface antigens of this species and those from the related lymphatic filariae, Brugia malayi and B. pahangi. All species bear a prominent Mr 29 000 surface antigen but among the differences observed were the strongly labelled molecules with Mr 58 000 and 67 000 in W. bancrofti which are extremely faint in the Brugia species. The [35S]methionine label was effectively incorporated into somatic parasite proteins in vitro although it was not possible to identify any secreted proteins in this way. The antigenicity of these products was investigated using a variety of sera from homologous and heterologous infections and the immunoprecipitation patterns highlighted particular differences between somatic proteins of male and female worms. One secreted antigen was detected, however, by virtue of its phosphorylcholine epitopes, in the culture medium of mixed adult worms; medium from male W. bancrofti adults was negative although homogenates of either sex of adult W. bancrofti were strongly positive in the same system.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 3 , December 1986 , pp. 559 - 569
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bolton, A. E. & Hunter, W. M. (1973). The labelling of proteins to high specific radioactivities by conjugation to a 125I-acylating agent. The Biochemical Journal 133, 529–39.CrossRefGoogle ScholarPubMed
Dissanayake, S. & Ismail, M. M. (1980). Antigens of Setaria digitata: cross-reaction with surface antigens of Wuchereria bancrofti microfilariae and serum antibodies of W. bancrofti-infected subjects. Bulletin of the World Health Organization 58, 649–54.Google ScholarPubMed
Forsyth, K. P., Mitchell, G. F. & Copeman, D. B. (1984). Onchocerca gibsoni: increase of circulating egg antigen with chemotherapy in bovines. Experimental Parasitology 58, 41–55.CrossRefGoogle ScholarPubMed
Forsyth, K. P., Spark, R., Kazura, J., Brown, G. V., Peters, P., Heywood, P., Dissanayake, S. & Mitchell, G. F. (1985). A monoclonal antibody-based immunoradiometric assay for detection of circulating antigen in Bancroftian filariasis. Journal of Immunology 134, 1172–7.CrossRefGoogle ScholarPubMed
Gualzata, M., Weiss, N. & Heuser, C. H. (1986). Dipetalonema viteae: Phosphorylcholine and non-phosphorylcholine antigenic determinants in infective larvae and adult worms. Experimental Parasitology 61, 95–102.CrossRefGoogle ScholarPubMed
Kaushal, N. A., Hussain, R., Nash, T. E. & Ottesen, E. A. (1982). Identification and characterisation of excretory-secretory products of Brugia malayi adult filarial parasites. Journal of Immunology 129, 338–43.CrossRefGoogle ScholarPubMed
Kaushal, N. A., Hussain, R. & Ottesen, E. A. (1984). Excretory-secretory and somatic antigens in the diagnosis of human filariasis. Clinical and Experimental Immunology 56, 567–76.Google ScholarPubMed
Kharat, I., Kaliraj, P., Ghirnikar, S. N. & Harinath, B. C. (1981). Detection and diagnostic utility of Wuchereria bancrofti microfilariae. Indian Journal of Experimental Biology 19, 564–5.Google ScholarPubMed
Maizels, R. M., Partono, F., Oemijati, S., Denham, D. A. & Ogilvie, B. M. (1983 a). Cross-reactive surface antigens on three stages of Brugia malayi, B. pahangi and B. timori. Parasitology 87, 249–63.CrossRefGoogle ScholarPubMed
Maizels, R. M., Partono, F., Oemijati, S. & Ogilvie, B. M. (1983 b). Antigenic analysis of Brugiatimori, a filarial nematode of man: initial characterisation by surface radioiodination and evaluation of diagnostic potential. Clinical and Experimental Immunology 51, 269–77.Google ScholarPubMed
Maizels, R. M., Philipp, M., Dasgupta, A. & Partono, F. (1984). Human serum albumin is a major component on the surface of microfilariae of Wuchereria bancrofti. Parasite Immunology 6, 185–90.CrossRefGoogle Scholar
Maizels, R. M., Denham, D. A. & Sutanto, I. (1985 a). Secreted and circulating antigens of the filarial parasite Brugia pahangi: analysis of in vitro released components and detection of parasite products in vivo. Molecular and Biochemical Parasitology 17, 277–88.CrossRefGoogle ScholarPubMed
Maizels, R. M., Sutanto, I., Gomez-Priego, A., Lillywhite, J. & Denham, D. A. (1985 b). Specificity of surface molecules of adult Brugia parasites: Cross-reactivity with antibody from Wuchereria, Onchocerca and other human filarial infections. Tropical Medicine and Parasitology 36, 233–7.Google ScholarPubMed
Maizels, R. M., Burke, J., Sutanto, I., Purnomo, & Partono, F. (1986). Secreted and surface antigens from larval stages of Wuchereria bancrofti, the major human lymphatic filarial parasite. Molecular and Biochemical Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Malhotra, A. & Harinath, B. C. (1984). Comparative efficiency of Wuchereria bancrofti microfilarial and larval excretory-secretory antigens in ELISA for the diagnosis of tropical eosinophilia and bancroftian filariasis. Indian Journal of Experimental Biology 22, 520–2.Google ScholarPubMed
Marshall, E. & Howells, R. E. (1985). An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125Iodine. Molecular and Biochemical Parasitology 15, 295–304.CrossRefGoogle ScholarPubMed
Markwell, M. A. K. & Fox, C. F. (1978). Surface-specific iodination of membrane proteins of viruses and eukaryotic cells using l,3,4,6-tetra-chloro-3a,6a-diphenylglycouril. Biochemistry 17, 4807–17.CrossRefGoogle Scholar
Ottesen, E. A. (1984). Immunological aspects of lymphatic filariasis and onchocerciasis in man. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 9–18.CrossRefGoogle ScholarPubMed
Palmieri, J. R., Connor, D. H., Purnomo, , Dennis, D. T. & Marwoto, H. (1982). Experimental infection of Wuchereria bancrofti in the silvered leaf monkey Presbytis cristatus Eschscholtz, 1821. Journal of Helminthology 56, 243–5.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E., Philipp, M. & Ogilvie, B. M. (1981). Characterisation of surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–52.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E. & Clark, N. W. T. (1983). Stage specific secreted and somatic antigens of Trichinella spiralis. Molecular and Biochemical Parasitology 9, 319–27.Google ScholarPubMed
Partono, F. (1984) Filariasis in Indonesia: clinical manifestations and basic concepts of treatment and control. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 9–12.CrossRefGoogle ScholarPubMed
Pery, P., Petit, A., Poulain, J. & Luffau, G. (1974). Phosphorylcholine bearing components in homogenates of nematodes. European Journal of Immunology 4, 637–9.CrossRefGoogle ScholarPubMed
Philipp, M., Gomez-Priego, A., Parkhouse, R. M. E., Davies, M. W., Clark, N. W. T., Ogilvie, B. M. & Beltran-Hernandez, F. (1984). Identification of an antigen of Onchocerca volvulus of possible diagnostic use. Parasitology 89, 295–309.CrossRefGoogle ScholarPubMed
Reddy, M. V. R., Piessens, W. F. & Harinath, B. C. (1984). Monoclonal antibodies against Wuchereria bancrofti microfilarial excretory–secretory antigens. Journal of Bioscience 6, 717–22.CrossRefGoogle Scholar
Ridley, D. S. & Hedge, E. C. (1977). Immunofluorescent reactions with microfilariae. 2. Bearing on host-parasite relations. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 522–5.CrossRefGoogle ScholarPubMed
Sasa, M. (1976). Human Filariasis. A Global Survey of Epidemiology and Control. Baltimore: University Park Press.Google Scholar
Selkirk, M. E., Denham, D. A., Partono, F., Sutanto, I. & Maizels, R. M. (1986). Molecular characterization of antigens of lymphatic filarial parasites. In Parasites and Molecular Biology: Applications of New Techniques, vol. 23 (ed. Simpson, A. J. G.). Parasitology 92 Suppl. S15–S38.Google Scholar
Sugane, K. & Oshima, T. (1983). Activation of complement in C-reactive protein positive sera by phosphorylcholine-bearing component isolated from parasite extract. Parasite Immunology 5, 385–95.CrossRefGoogle ScholarPubMed
Sutanto, I., Maizels, R. M. & Denham, D. A. (1985). Surface antigens of a filarial nematode: analysis of adult Brugia pahangi surface components and their use in monoclonal antibody production. Molecular and Biochemical Parasitology 15, 203–14.CrossRefGoogle ScholarPubMed
Taylor, D. W., Goddard, J. M. & McMahon, J. E. (1986). Surface components of Onchocerca volvulus. Molecular and Biochemical Parasitology 18, 282–300.CrossRefGoogle ScholarPubMed