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Mixed light imaging system for recording bioluminescence behaviours

Published online by Cambridge University Press:  11 May 2009

Edith A. Widder
Edith A. Widder
Affiliation:
Harbor Branch Oceanographic Institution, 5600 Old Dixie Highway, Fort Pierce, Florida 34946, USA
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Extract

Video recordings of bioluminescence have depended on the use of image-intensified systems, which generally suffer from poor resolution and image persistence. These characteristics, coupled with the restriction that any illumination must be dimmer than the bioluminescence, have complicated efforts to record behaviours associated with bioluminescent displays. The Mixed Light Imaging System (Mlis), described here, uses (1) an intensified video camera, (2) an infra-red (IR) sensitive video camera (3) a dichroic beam splitter, (4) strobed IR illumination and (5) a video mixer to record stop-action images of organisms superimposed on intensified images of their bioluminescence. With the MLIS, behaviours associated with bioluminescent emissions, including rapid escape responses, were recorded from the mesopelagic copepods Euaugaptilus magnus and Gaussia princeps.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 72 , Issue 1 , February 1992 , pp. 131 - 138
Copyright
Copyright © Marine Biological Association of the United Kingdom 1992

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