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Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil

Published online by Cambridge University Press:  19 October 2009

H. G. Pereira ,
Rosete S. Azeredo ,
J. P. G. Leite ,
J. A. N. Candeias ,
Maria L. Rácz ,
A. C. Linhares ,
Yvonne B. Gabbay  and
J. R. Trabulsi
H. G. Pereira
Affiliation:
Department of Virology, Osivaldo Cruz Foundation, Rio de Janeiro, Brazil
Rosete S. Azeredo
Affiliation:
Department of Virology, Osivaldo Cruz Foundation, Rio de Janeiro, Brazil
J. P. G. Leite
Affiliation:
Department of Virology, Osivaldo Cruz Foundation, Rio de Janeiro, Brazil
J. A. N. Candeias
Affiliation:
Department of Biomedical Sciences, University of São Paulo, S. Paulo, S.P.Brazil
Maria L. Rácz
Affiliation:
Department of Biomedical Sciences, University of São Paulo, S. Paulo, S.P.Brazil
A. C. Linhares
Affiliation:
Evandro Chagas Institute, Belem, Pará, Brazil
Yvonne B. Gabbay
Affiliation:
Evandro Chagas Institute, Belem, Pará, Brazil
J. R. Trabulsi
Affiliation:
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, S. Paulo, S.P., Brazil
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Human rotaviruses from the states of Rio de Janeiro, São Paulo and Pará of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86·8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by enzyme immunoassay. Of the RNA-positi ve samples, 18 (13·0%) were classified as subgroup 1, 94 (68·1%) as subgroup 2, and 26 (18·8%) could not be classified due to absence of visible bands 10 and 11. Subgroup 2 was more frequent in the three states. All strains of subgroup 1 detected in Rio de Janeiro were associated with a single short-lived school outbreak. All strains of subgroup 1 resembled each other in electrophoretie pattern, irrespective of geographical origin, although minor differences could be detected by co-electrophoresis. Subgroup 2, on the other hand, showed a great degree of electrophoretic heterogeneity and could be divided into several sub-categories.

Type
Research Article
Information
Journal of Hygiene , Volume 90 , Issue 1 , February 1983 , pp. 117 - 125
Copyright
Copyright © Cambridge University Press 1983

References

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