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A Study of the Intestinal Flora under normal and abnormal Conditions

Published online by Cambridge University Press:  15 May 2009

Leonard S. Dudgeon
Leonard S. Dudgeon
Affiliation:
(Professor of Pathology, University of London, Director of Pathology, St Thomas's Hospital.)
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(1) Each sample of faeces for these investigations was dried on unglazed porous tiles by the method introduced by Dudgeon. The dried feacal powder was added to the various solid and liquid media used for the aerobic and anaerobic examinations. By this method a large quatity of the faeces was examined, blood agar and boiled blood agar media were used as a routine procedure, and it was unnecessary to employ media which hindered the growth of one organism at the expense of another.

(2) It is essential to control the intake of food during the bacteriological investigations of the faecal flora.

(3) Among hearlthy individuals, dietetic errors, excess of alcohol, and active purgation may temporarily alter the bacterial content of the faeces and increase the numbers of bacteria present, even to the same extent as among patients suffering from lesions of the intestinal tract.

(4) Septic infections of the mouth, throat, respiratory, and urinary tracts may give abnormal bacteriological findings in the faecal flora.

(5) Film preparations of the faeces should be made in every instance, but care must be exercised that the correct technique is employed.

(6) Experience has shown that several media are essential for the aerobic and anaerobic investigations of the faecal flora in each case.

(7) Spirochaetes were found in the faeces in 7 per cent. of the cases and usually in large numbers. There was no evidence to show that they had produced a pathological process in the bowel wall. Short coarse and long delicate spirochaetes occurred. Experience has shown that it is necessary to examine the mouth and respiratory tract in such cases for spirochaetal infection.

(8) Streptococci. One hundred and fifteen cultures of streptococci isolated from the faeces in 50 cases of various kinds were investigated. Eighty-two out of the 115 cultures proved to be enterococci. The morphology of this organism is its most striking characteristic. Twenty-one out of the 82 strains were haemolytic, of which 14 were thermostable and 13 acidified mannite. Many strains believed to be haemolytic streptococci were found on more detailed examination to be enterococci.

Nineteen strains of long-chained streptococci were isolated, out of which 6 were haemolytic, all were thermolabile, but only 1 acidified mannite.Fourteen strains of medium and short-chained streptococci were isolated.Five of these strains were haemolytic, 2 were thermolabile and 8 acidified mannite.

Haemolytic streptococci have been cultivated from the tonsils or sputum with the same cultural reactions and heat resistance as among those found in the patient's faeces, sometimes in large numbers.

Streptococci with similar cultural and haemolytic reactions and heat resistance have been cultivated from the faeces of normal individuals and from patients suffering from various infective processes.

(9) Haemolytic colon bacilli. These occurred in the faeces under varying conditions in 6 per cent, of the cases, and in most instances were found in abundance. In urinary infections the same haemolytic strains may occur in the faeces and urinary pus. Some cases of toxaemia due to the presence of haemolytic colon bacilli in the faeces in abundance, have improved with specific vaccine treatment to a degree beyond that met with in any other form of intestinal infection.

(10) The mucus capsulatus group occurred in 5·5 per cent. of the cases, usually in patients suffering from an abnormal condition. of the intestinal tract.

(11) Slow lactose fermenters were cultivated in 2 per cent. of the cases. They have been found occasionally in the faeces of patients suffering from acute urinary fever due to the same bacillus.

(12) Staphylococci. The Staphylococcus aureus and albus were present in the faeces in this series in about 35 per cent, in adults. They occurred in large numbers in some cases of diarrhoea, but especially in patients suffering from pulmonary infections, and their presence in the faeces in typhoid fever may be of considerable importance. When staphylococci, especially S. aureus, occur in the faeces in large numbers, or persist as shown by multiple examinations, then it is advisable to examine the patient for an inflammatory focus.

(13) Diphtheria-like bacilli were isolated from the faeces in 6 per cent, of the cases. They were larger and coarser than true diphtheria bacilli, but gave a well-marked reaction with one of the modifications of Neisser's stain. They were non-pathogenic to guinea-pigs. Their presence in the faeces appears to be harmless, but they are of importance in children suffering from enlarged tonsils owing to errors of diagnosis which may arise in connection with true diphtheria bacilli.

(14) Anaerobes. B. welchii was cultivated from the faeces in 35 per cent. of the total cases. Robertson's heart muscle medium was the most effective for primary culture. Occasionally gangrenous infections due to B. welchii occurred. There was no positive evidence to show, however, that B. welchii produced acute gastro-enteritis, or acute colitis, but its relationship to certain infections and toxaemias is discussed.

(15) Three types of intestinal flora are referred to: (1) fermentative or saccharolytic, (2) putrefactive or proteolytic, (3) facultative or normal. The importance of diet in all bacteriological investigations of the faeces is established, while an abnormal faecal flora may be rendered normal by a simple diet. The value of lactose, dextrin, and milk for the treatment of intestinal disorders is fully discussed.

(16) Intestinal antiseptics. My observations with so-called intestinal antiseptics has led me to believe that on the whole they are devoid of bactericidal action on the intestinal flora of man. Dimol and colloidal kaolin have been proved to be exceptions in some instances.

(17) The pathology of intestinal toxaemia based on the work of the American investigators by means of closed intestinal loops is considered.

(18) The characteristics of the acidophilus group of organisms is referred to, including their value in controlling the intestinal flora of man, and for the treatment of intestinal disorders by means of acidophilus milk.

Type
Research Article
Information
Journal of Hygiene , Volume 25 , Issue 2 , July 1926 , pp. 119 - 141
Copyright
Copyright © Cambridge University Press 1926

References

REFERENCES

Azmar, P. (1924). Aerobic bacilli with terminal spores in the intestinal flora of man. Compt. Rend. Soc. de Biol. Paris, xc. 674–75.Google Scholar
Bassler, A. and Lutz, J. R. (1922). Bacillus acidophilus. (Its very limited value in intestinal disorders.) Journ. Amer. Med. Assoc. lxxix. 607.Google Scholar
Cannon, ,Paul, R. (1921). The effect of Diet on the Intestinal Flora. Journ. Infect. Dis. xxix. 369.CrossRefGoogle Scholar
Cannon, , Paul, R. (1924). A biological study of aciduric bacteria. Journ. Infect. Dis. xxxiv. 227.CrossRefGoogle Scholar
Chaplin, H. A., Fulmer, H. C. and Barney, C. O. (1923). Studies on the therapeutic application of bacillus acidophilus milk. Journ. Amer. Med. Assoc. lxxx. 1896.CrossRefGoogle Scholar
Cushing, , Harvey, and Livingood, L. E. (1900). Experimental and surgical notes upon the bacteriology of the upper portion of the alimentary canal, with observations on the establishment there of an amicrobic state as a preliminary to operative procedures on the stomach and small intestine. In “Contributions to the Science of Medicine, dedicated by his pupils to William Henry Welch, upon the 25th anniversary of his Doctorate.” Johns Hopkins Hospital Reports ix.Google Scholar
Dible, J. Henry (1921). The enterococcus and the faecal streptococci. Their properties and relations. Journ. Pathol. and Bacteriol. xxiv. 1–35.Google Scholar
Dragstedt, L. R., Moorhead, J. J. and Burcky, F. W. (1917). Intestinal Obstruction. An Experimental Study of the Intoxication in Closed Intestinal Loops. Journ. Exper. Med. xxv. 421.CrossRefGoogle Scholar
Dragstedt, L. R., Cannon, P. R. and Dragstedt, C. A. (1922). Factors controlling the intestinal bacteria. Journ. Infect. Dis. xxxi. 209.CrossRefGoogle Scholar
Dragstedt, L. R. and , C. A. and Nisbet, O. M. (1922). Intestinal antiseptics. Effect of antiseptics on a type of experimental intestinal toxaemia. Journ. of Lab. and Clin. Med. St Louis, viii. 190–193.Google Scholar
Dudgeon, L. S., Wordley, E. and Bawtree, F. (1921 and 1922). On Bacillus coli infections of the urinary tract, especially in relation to haemolytic organisms. Journ. Hygiene, xx. and xxi. 137–164.CrossRefGoogle Scholar
Dudgeon, Leonard S. (1924). Acute infection of the urinary tract due to a special group of haemolytic bacilli. Journ. Hygiene xxii. 348–354.CrossRefGoogle Scholar
Dudgeon, L. S. and Jewesbury, R. C. (1924). The bacteriology of human milk. Journ. Hygiene xxiii. 64–76.CrossRefGoogle Scholar
Dudgeon, Leonard S. and Mitchiner, P. H. (1924). The Bacteriological and Pathological Examination of the Vermiform Appendix in the first thirty hours of acute Appendicitis. Brit. Journ. Surgery, xi. No.44.Google Scholar
Dudgeon, L. S. and Bamforth, J. (1925). On Staphylococcal precipitin reactions in cases of acute and chronic infections and also in serum sickness. Journ. Hygiene, xxiii. 375–388.Google Scholar
Gilbert, and Dominici, (1904). Action du régime lacté sur le microbisme du tube digestif. Bull. Soc. de Biol. Paris, Avril 14, 277.Google Scholar
Henneberg, W. (1922). Untersuchungen über die Darmflora des Menschen mit besonderer Berücksichtigung der jodophilen Bakterien im Menschen und Tierarten sowie im Komptostdünger. Centraibl. f. Bakteriol, 2, LV, 243–281.Google Scholar
Hindle, E. (1925). A Leptospira which has been found in London tap-water. Trans. Roy. Soc. Trop. Med. and Hygiene, xix, Nos. 2 and 2.Google Scholar
Houston, Alexander (1925). Metropolitan Water Board. Nineteenth Annual Report. London.Google Scholar
Hudson, N. Paul and Parr, L. W. (1924). The relation of reaction of intestinal contents to diet and flora. Journ. Inf. Dis. Chicago, xxxiv. 621–624.CrossRefGoogle Scholar
Hunter, C. A. (i. 1924). A preliminary report of media for the cultivation of the lacto-bacillus group. (Sci. Proc. of Soc. of Amer. Bacteriologists.) Abstracts of Bacterial. viii. No. 2.Google Scholar
Kopeloff, N. (3. iii. 1923). Is the action of Bacillus acidophilus a strictly bacteriological phenomenon? Abstracts of Bacterial. 03 3rd.Google Scholar
Kopeloff, N. and Cheney, C. O. (19. viii. 1922). Studies on the therapeutic effect of Bacillus acidophilus milk and lactose. Journ. Amer. Med. Assoc. lxxix. 609.Google Scholar
Lockhart, L. P. (1924). The bacteriological examinations of the faeces in four cases of typhoid fever, made at frequent intervals for a period of one month. Journ. Hygiene, xxiii. 106–116.CrossRefGoogle Scholar
McIntosh, J., Warwick, James W. and Lazarus Barlow, P. (1924). An investigation into the aetiology of Dental Caries. The Biological characteristics and distribution of B. acidophilus odontolyticus. Brit. Journ. of Exp. Pathol. v. 175.Google Scholar
Morris, G. B., Porter, R. L. and Meyer, K. F. (1919). The Bacteriological analysis of the faecal flora of children. Journ. Infect. Dis. xxv. 349–377.CrossRefGoogle Scholar
Nuttall, G. H. P. and Graham-Smith, G. S. (1908). The Bacteriology of Diphtheria. Cambridge University Press.Google Scholar
Pane, N. (16. viii. 1919). The normal intestinal flora in adults. Riforma Med. Naples, xxxv. 33.Google Scholar
Report of the Anaerobic Committee (12. iv. 1918). The Demonstration of Anaerobes in Wounds of recent date. Medical Research Committee.Google Scholar
Tavel, and Lanz, . Ueber die Aetiologie der Peritonitis. Mittheil. a. Klin. u. Instituten der Schweiz, Basel.Google Scholar
Torrey, J. C. (1915). The faecal flora of typhoid fever and its reaction to various diets. Journ. Infect. Dis. xvi. 72–108.Google Scholar
Torrey, J. C. (1918). Journ. Med. Research, xxxix. 415.Google Scholar
Veillon, and Zuber, (vii. 1898). Recherches sur quelques microbes strictement anérobes et leur role en pathologie. Arch, de Méd. expér.Google Scholar
Weinberg, M., Azmar, P. and Duthie, M. (1924). [Anaerobes of the intestinal flora of man having terminal spores.] Compt. Rend. Soc. de Biol. Paris, XCI, 621.Google Scholar
Wordley, E. (1921). A new method for the isolation of organisms from faeces and sputum with some observations on haemolytic streptococci in faeces obtained by this method. Journ. of Hygiene, xx. 60–68.CrossRefGoogle Scholar
Wordley, E. (1922). Observations on haemolytic streptococci in the faeces of patients suffering from scarlet fever and nephritis. Lancet, ii, 610.CrossRefGoogle Scholar