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Evidence for an interaction between cationic and neutral amino acids at the blood-facing aspect of the lactating rat mammary epithelium

Published online by Cambridge University Press:  01 June 2009

D. T. Calvert  and
D. B. Shennan
D. T. Calvert
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
D. B. Shennan
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
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Summary

The transport of lysine by perfused lactating rat mammary tissue has been examined using a rapid, paired-tracer dilution technique. This experimental approach allowed the characteristics of lysine transport across the blood-facing aspect of the mammary epithelium to be studied. The clearance of lysine from the perfusate was influenced by the extracellular lysine concentration in a fashion consistent with the presence of carrier-mediated transport. Replacing extracellular Na+ with N-methyl-D-glucamine had no significant effect on lysine transport. Lysine uptake was inhibited by extracellular leucine and glutamine but not by α-(methylamino)isobutyric acid. Leucine interacted with lysine transport under Na+-free conditions. It appears that the system for cationic acid transport which is situated in the blood-facing aspect of the lactating rat mammary epithelium may also accept neutral amino acids as substrates.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 1 , February 1996 , pp. 25 - 33
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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References

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