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Induction of mutations in the zebrafish with ultraviolet light

Published online by Cambridge University Press:  14 April 2009

David Jonah Grunwald  and
George Streisinger
David Jonah Grunwald
Affiliation:
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403
George Streisinger
Affiliation:
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403
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Summary

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Recessive lethal germline and specific locus somatic mutations were induced efficiently in the zebrafish by exposure of mature sperm to UV light. Mutagenesis of sperm yielded mosaic individuals: clones bearing novel mutations represented approximately 12–25% of the haploid germ cells and 25–50 % of the somatic tissue. Simple methods are described for the reliable identification and propagation of newly arising developmental mutations in zebrafish.

Type
Research Article
Information
Genetics Research , Volume 59 , Issue 2 , April 1992 , pp. 93 - 101
Copyright
Copyright © Cambridge University Press 1992

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