Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-05-26T18:42:56.563Z Has data issue: false hasContentIssue false
  • English
  • Français

Recombination within the am gene of Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

J. R. S. Fincham  and
J. A. Pateman
J. R. S. Fincham
Affiliation:
John Innes Institute, Bayfordbury, Hertford, Herts.

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Maps have been made showing the order of am mutant sites using (a) frequencies of am+ recombinants from crosses between am mutants and (b) the modes of distribution of the am+ recombinants among the two parental and two recombinant classes with respect to the flanking markers inos and sp.

It is possible to arrange the sites in an order such that, in almost all the crosses yielding useful numbers of am+ recombinants, the sp allele originally in coupling with the distal am mutant site occurs in the majority of the recombinants. No such consistent pattern was found with respect to the inos marker. The order obtained by reference to sp agreed with the best order deducible from recombination frequencies.

The data are consistent with the hypothesis that am+ recombinants arise by a process of gene conversion, that there is a gradient of conversion frequencies from the right (inos or distal) end of the gene to the left (sp or proximal) end, and that conversion tends to be associated (though less than 50% of the time) with crossing-over, especially on the distal side.

There is no obvious relationship between the map position of a given am mutant and the properties of the protein product of the mutant gene.

Type
Research Article
Information
Genetics Research , Volume 9 , Issue 1 , February 1967 , pp. 49 - 62
Copyright
Copyright © Cambridge University Press 1967

References

REFERENCES

Case, M. E. & Giles, N. H. (1958). Recombination mechanisms at the pan-2 locus in Neurospora crassa. Cold Spring Harb. Symp. quant. Biol. 23, 119.CrossRefGoogle ScholarPubMed
Fincham, J. R. S. (1950). Mutant strains of Neurospora deficient in aminating ability. J. biol. Chem. 182, 61.CrossRefGoogle Scholar
Fincham, J. R. S. (1958). The role of chromosomal loci in enzyme formation. Proc. X Int. Congr. Genet. I, 355–363. Toronto: Univ. Press.Google Scholar
Fincham, J. R. S. (1962). Genetically determined multiple forms of glutamic dehydrogenase in Neurospora crassa. J. molec. Biol. 4, 257.CrossRefGoogle ScholarPubMed
Fincham, J. R. S. & Stadler, D. R. (1965). Complementation relationships of Neurospora am mutants in relation to their formation of abnormal varieties of glutamate dehydrogenase. Genet. Res. 6, 419.CrossRefGoogle ScholarPubMed
Holliday, R. (1964). A mechanism for gene conversion in fungi. Genet. Res. 5, 282.CrossRefGoogle Scholar
Lissouba, P., Mousseau, J., Rizet, G. & Rossignol, J. L. (1962). Fine structure of genes in the Ascomycete Ascobolus immersus. Adv. Genet. 11, 343.CrossRefGoogle Scholar
Murray, N. E. (1963). Polarized recombination and fine structure within the me-2 gene of Neuroapora crassa. Genetics, 48, 1163.CrossRefGoogle ScholarPubMed
Pateman, J. A. (1957). Back-mutation studies at the am locus of Neurospora crassa. J. Genet. 55, 444.CrossRefGoogle Scholar
Pateman, J. A. (1958). Aberrant recombination at the am locus in Neurospora crassa. Nature, Lond. 181, 1605–1606.CrossRefGoogle Scholar
Pateman, J. A. (1960). High negative interference at the am locus in Neurospora crassa. Genetics, 45, 839.CrossRefGoogle Scholar
Pritchard, R. H. (1955). The linear arrangement of a series of alleles of Aspergillus nidulans. Heredity, Lond. 9, 343.CrossRefGoogle Scholar
Pritchard, R. H. (1960). Localized negative interference and its bearing on models of gene recombination. Genet. Res. 1, 1.CrossRefGoogle Scholar
Roberts, D. B. & Pateman, J. A. (1964). Immunological studies of amination deficient strains of Neuroapora crassa. J. gen. Microbiol. 34, 295–305.CrossRefGoogle Scholar
Smith, B. R. (1965). Interallelic recombination at the his-5 locus in Neurospora crassa. Heredity, Lond. 20, 257.CrossRefGoogle ScholarPubMed
Stadler, D. R. & Towe, A. M. (1963). Recombination of allelic cysteine mutants in Neurospora. Genetics, 48, 1323.CrossRefGoogle ScholarPubMed
Vogel, H. J. (1956). A convenient growth medium for Neurospora (medium N). Microb. Genet. Bull. 13, 42.Google Scholar
Westergaard, M. & Mitchell, H. K. (1947). Neurospora. V. A synthetic medium favouring sexual reproduction. Am. J. Bot. 34, 573.CrossRefGoogle Scholar
Whitehouse, H. L. K. & Hastings, P. J. (1965). The analysis of genetic recombination on the polaron hybrid DNA model. Genet. Res. 6, 27.CrossRefGoogle ScholarPubMed