Pseudomonas rhodesiae sp. nov., a New Species Isolated from Natural Mineral Waters

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Summary

Deoxyribonucleic acid relatedness studies (S1 nuclease method) showed that 7 strains of the cluster IIa previously described by Elomari et al. (J. Appl. Bacteriol 78, 71-81, (1995)) formed a homogenous deoxyribonucleic acid hybridization group. This group of strains forms a new species: Pseudomonas rhodesiae sp. nov. A total of 67 strains representing known or partially characterized sprecies of the genus Pseudomonas had 7 to 48 % DNA hybridization with Pseudomonas rhodesiae. This new species composed of strains isolated from natural mineral waters, were Gram negative, rod shaped, and motile by means of a single polar flagellum. They were not able to accumulate poly-γ-hydroxybutyrate, were capable of respiratory but not fermentative metabolism. They grew at 4°C but not a 41°C, produced fluorescent pigment, catalase, cytochrome oxidase and lecithinase, and possessed arginine dihydrolase system. They did not hydrolyse gelatin and starch, and were able to use glucose, trehalose, 2-ketogluconate, inositol, L-valine and γ-alanine. They possessed L-pyrrolidone arylamidase, L-histidyl-L-serine arylamidase but did not have osidase, esterase = C12, esterase = C14, esterase = C16, and glycyl-L-tryptophane arylamidase. The average guanine + cytosine (G + C) content of the deoxyribonucleic acid was 59 ± 1 mol%. The type strain of P. rhodesiae has been deposited as CIP 1046641. The clinical significance of Pseudomonas rhodesiae is unknown.

References (31)

  • D. De Vos et al.

    Detection of the outer membrane lipoprotein I and its gene in fluorescent and non-fluorescent pseudomonads; implications dor taxonomy and diagnosis

    J. Gen. Microbiol

    (1993)
  • M. Elomari et al.

    A numerical taxonomic study of fluorescent Pseudomonas strains isolated from natural mineral waters

    J.Appl. Bacteriol

    (1995)
  • M. Elomari et al.

    DNA relatedness among strains of Pseudomonas isolated from natural mineral waters and proposal of Pseudomonas veronii sp. nov

    Int. J. Syst. Bacteriol

    (1996)
  • L. Gardan et al.

    DNA relatedness among the pathovar strains of Pseudomonas syringae subsp

    savastanoi Janse (1982) and proposal of Pseudomonas savastanoi sp. nov. Int. J. Syst. Bacteriol

    (1992)
  • W.D. Gould et al.

    New selective media for enumeration and recovery of fluorescent pseudomonads from various habitats

    Appl. Environ. Microbiol

    (1985)
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