High-performance size-exclusion chromatographic procedure for the determination of fluoresceinyl isothiocyanate dextrans of various molecular masses in biological media
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Assay considerations for fluorescein isothiocyanate-dextran (FITC-d): an indicator of intestinal permeability in broiler chickens
2021, Poultry ScienceCitation Excerpt :For 4-kDa FITC-d, the fluorescence measurement is conducted with a microplate reader at an excitation wavelength of 485 nm and the emission wavelength of 528 nm (Kuttappan et al., 2015ab). A previous study reported that FITC-d were stable for more than three days at 37°C in biological media (Kurtzhals et al., 1989). In poultry studies, some researchers conducted the FITC-d measurements on the same day of the sampling (Bortoluzzi et al., 2019a; Teng et al., 2020), whereas other researchers have kept serum samples at –20 C until further analysis (Gilani et al., 2017b).
Dextrans for targeted and sustained delivery of therapeutic and imaging agents
2000, Journal of Controlled ReleaseHigh-performance size-exclusion chromatographic analysis of dextran-methylprednisolone hemisuccinate in rat plasma
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2000, Journal of Pharmaceutical SciencesCitation Excerpt :A 250‐mm × 4.6‐mm Microsphere GPC 150 size exclusion column (7 μ) obtained from Altech Associates (Deerfield, IL) was used for the quantitation of the vitreous and aqueous samples. The size exclusion analytical method was adapted from Kuetzhals et al15 with modifications. The mobile phase consisted of 0.01 M ammonium phosphate buffer (pH 7.0).