Role of cytochrome b5 reductase on the antioxidant function of coenzyme Q in the plasma membrane

doi:10.1016/S0098-2997(97)00015-0

Molecular Aspects of Medicine

Volume 18, Supplement 1, 1997, Pages 7-13

https://doi.org/10.1016/S0098-2997(97)00015-0 Get rights and content

Abstract

Cytochrome b₅ reductase purified from liver plasma membrane reduces coenzyme Q (CoQ) in reconstituted liposomes in the absence of cytochrome b₅. Both CoQ and its reductase are responsible for the reduction of the ascorbate free radical at the cell surface. Thus, NADH-CoQ reductase represents a partial reaction of NADH-AFR reductase in the plasma membrane. Cytochrome b₅ reductase maintains CoQ and ascorbate in their reduced state to support antioxidations. Reduced CoQ prevents lipid peroxidation in liposomes and plasma membranes. Also, oxidized CoQ can prevent lipid peroxidations in the presence of cytochrome b₅ reductase and NADH. Addition of CoQ to intact cells prevents serum withdrawal-induced lipid peroxidation and apoptosis. The prevention of apoptosis by CoQ is independent of the bcl-2 protein content in the cell. Antioxidants that act at the plasma membrane as CoQ and ascorbate would represent a first barrier to protect lipids from oxidative stress and subsequent apoptosis. Cytochrome b₅ reductase is then an enzyme leading this function at the plasma membrane. These data support the idea that when the plasma membrane barrier fails, bcl-2 protein would be required to prevent cell death.

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Role of cytochrome b5 reductase on the antioxidant function of coenzyme Q in the plasma membrane

Abstract

Biochimica Biophysica Acta

Free Radicals in Biology and Medicine

Cell

Biochemistry and Biophysics Research Communication

Biochimica Biophysica Acta

The role of ascorbate in antioxidant protection of biomembranes: interaction with vitamin E and coenzyme Q

Journal of Bioenergetics and Biomembranes

NADH-ascorbate free radical reductase of rat liver plasma membranes: kinetics and lectin inhibition

Epithelia

Plasma membrane redox enzymes

Reactive oxygen species and antioxidant vitamins: mechanisms of action

American Journal of Medicine

Antioxidant ascorbate is stabilized by NADH-coenzyme Q10 reductase in plasma membrane