A murine model of IgE-mediated cow’s milk hypersensitivity☆,☆☆,★
Section snippets
METHODS
Female C3H/HeJ mice, 3 weeks of age (immediately after weaning), were purchased from the Jackson Laboratory (Bar Harbor, Me) and maintained on regular mouse chow under specific pathogen-free conditions. Guidelines for the care and use of the animals were followed.25
Homogenized CM (GAF Seelig Inc) was used. CT was purchased from List Biological Laboratories, Inc (Campbell, Calif). Concanavalin (Con A) and albumin, human-dinitrophenyl (DNP-albumin) were purchased from Sigma (St Louis, Mo).
CM-specific IgE responses after intragastric CM sensitization
To investigate the kinetics of IgE production in the development of CMH, serum CM-specific IgE was monitored weekly by ELISA. Mice sensitized with the medium dose (1 mg/g) of CM plus CT developed significant (P < .01) increases in antigen-specific IgE by 3 weeks, which peaked at 6 weeks after the initial sensitization (Fig 1).
DISCUSSION
In this study we identified certain conditions required to effectively generate a mouse model of CMA by oral sensitization and challenge. This model exhibited the characteristics of type 1 hypersensitivity reactions. Symptoms of anaphylactic reactions were apparent 15 to 30 minutes after oral challenge. The symptoms involved several organ systems, including the skin and respiratory and gastrointestinal tracts, with the most severe reactions being fatal. Increased vascular leakage and intestinal
Acknowledgements
We thank Ludmilla Bardina, MS, for technical assistance, and Scott Sicherer, MD, for his assistance in the preparation of this manuscript.
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Supported by AI 24439 NIAID, NIH.
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Reprint requests: Xiu-Min Li, MD, Pediatric Allergy and Immunology, The Mount Sinai School of Medicine, Box 1198, One Gustave L. Levy Place, New York, NY 10029-6574.
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