Chromatographic characterization of a molecularly imprinted polymer binding theophylline in aqueous buffers

https://doi.org/10.1016/S0021-9673(97)00537-2Get rights and content

Abstract

A theophylline-binding polymer was prepared utilizing the technique of non-covalent molecular imprinting polymerization. The polymer obtained was packed in a high-performance liquid chromatography (HPLC) column, and the molecular recognition mechanism regulating the binding behavior was studied by frontal and zonal chromatography, determining the effects of pH and methanol on the affinity constant and concentration of the binding sites. The values of binding capacity found show thet ability of the imprinted polymer to bind theophylline in aqueous buffer, even if with reduced affinity with respect to the values reported in literature for the same polymer in an organic medium. Hydrogen bonding was confirmed as leading interaction in the recognition mechanism.

References (25)

  • I.A. Nicholls et al.

    Trends Biotechnol.

    (1995)
  • M. Kempe et al.

    J. Chromatogr. A

    (1995)
  • M. Kempe et al.

    J. Chromatogr. A

    (1995)
  • E. Hedborg et al.

    Sens. Act. A

    (1993)
  • B. Sellergren et al.

    J. Chromatogr. A

    (1993)
  • I.A. Nicholls et al.

    J. Chromatogr. A

    (1995)
  • K. Kasai et al.

    J. Chromatogr.

    (1986)
  • L.I. Andersson et al.

    J. Chromatogr.

    (1990)
  • G. Wulff

    Angew. Chem., Int. Ed. Engl.

    (1995)
  • J. Steinke et al.

    Adv. Polym. Sci.

    (1995)
  • K. Mosbach et al.

    Biotechnology

    (1996)
  • B. Sellergren

    Anal. Chem.

    (1994)
  • Cited by (55)

    View all citing articles on Scopus
    View full text