Effect of diabetes and hypothyroidism on the predominance of cardiac myosin heavy chains synthesized in vivo or in a cell-free system

A cDNA clone for mouse apolipoprotein E has been identified from a mouse liver cDNA library by a combination of differential colony hybridization and hybrid selection-translation. The identity of the clone was unambiguously established by partial sequencing and comparison with human apolipoprotein E nucleotide and amino acid sequences. In conjunction with an in vitro translation assay for apolipoprotein E, the clone has been used to examine the relative levels of apolipoprotein E mRNA in various tissues of the mouse and the regulation of apolipoprotein E synthesis in response to a diet rich in saturated fat and cholesterol. In the tissues examined, the clone was found to hybridize to a polyadenylated RNA species of approximately 1400 nucleotides. Of the tissues involved in lipoprotein synthesis, liver is very rich (about 1% of total) in apolipoprotein E mRNA while intestine contains only trace amounts. Appreciable levels of active apolipoprotein E mRNA (up to 10% of that in liver) are also detected in peripheral tissues not associated with lipoprotein synthesis, including lung, kidney, spleen, and heart. Thus, extrahepatic apolipoprotein E synthesis may contribute significantly to the levels present in plasma, and a possible function in "reverse cholesterol transport" is considered. When mice were placed on a high lipid diet there was no discernible change in the level of apolipoprotein E mRNA in liver or intestine, although the level of the circulating protein increased about 3-fold. We conclude that in mice the effect of diet on apolipoprotein E levels in blood does not result from induction of mRNA in these tissues.

In vitro transcription initiation by purified RNA polymerase I1 within the adenovirus 2 major late promoter region.

P. Salzman Helena Mishe, John N. Brady, Gerald Lancz, and Norman
Reconstitution of Escherichia coli thioredoxin reductase with 1-deazaFAD. Evidence for 1-deazaFAD C-4a adduct formation linked to the ionization of an active site base.

Michael E. O'Donnell and Charles H. Williams, J r
The interaction between Escherichia coli aspartokidine-adenine dinucleotide phosphate (reduced), an an-nase-homoserine dehydrogenase and 3-acetylpyrialog of NADPH.
Identification of mannose 6-phosphate receptors in rabbit alveolar macrophages. Virginia L. Shepherd, Hudson H. Freeze, Arnold L. Miller, and Philip D. Stahl Correlation between cytosolic free Ca2+ and insulin release in an insulin-secreting cell line.
Rho-dependent termination and concomitant NTPase activity requires a specific, intact RNA region.
Characterization of the Fe-S cluster in aconitase using low temperature magnetic circular dichroism spectroscopy. Comparison of coagulation factor Xa and des-(1-44)factor Xa in the assembly of prothrombinase.
William F. Skogen, Charles T. Esmon, and A. Chadwick Cox In uiuo studies of pyridine nucleotide metabolism in Escherichia coli and Saccharomyces cerevisiae by carbon-13 NMR spectroscopy.
Isolation and preliminary characterization of proteoglycan aggregates from cultured dermal fibroblasts.

Irwin A. Schafer, h t i f a Sitabkha, and Maureen Pandy
Cytochrome electron spin resonance line shapes, ligand fields, and components stoichiometry in ubiquinol-cytochrome c oxidoreductase.

Mary L. Standaert and Robert J. Pollet
Biochemical characterization of the hamster thy mutator gene and its revertants.

Marie Trudel, Tamsen Van Genechten, and Mark Meuth
Homocysteine in tissues of the mouse and rat. On the mechanism of the acid-catalyzed hydrolysis of uridine to uracil. Evidence for 6-hydroxy-5,6-dihydrouridine intermediates. in human fibroblasts by diethyl maleate and other

Shiro Bannai
Transport of cystine in isolated rat hepatocytes in primary culture.

Akira Takada and Shiro Bannai
Identification by electron spin resonance of free radicals formed during the oxidation of 4-hydroxyanisole catalyzed by tyrosinase.

Mark J. Nilges, Harold M. Swartz, and Patrick A. Riley
Biosynthesis of apolipoprotein C-I11 in rat liver and small intestinal mucosa.
Mark C. Blaufuss, Jeffrey I. Gordon, Gustav Schonfeld, Arnold W. Straws, and David H. Alpers Cooperativity in highly aggregated enzyme systems. A slow transition model for the pyruvate dehydrogenase complex from Escherichia coli.

Hans Bisswanger
Phosphorylation of rabbit liver glycogen synthase by multiple protein kinases.

Marcella Camici, Zafeer Ahmad, Anna A. DePaoli-Roach, and Peter J . Roach
Ligand effects on the phosphorylation state of hepatic phenylalanine hydroxylase.

Robert S. Phillips and Seymour Kaufman
Photochemically induced dynamic nuclear polarization study on microsomal NADPH-cytochrome P-450 reductase.

Hardesty Eric Wollny, Kenneth Watkins, Gisela Kramer, and Boyd
Partial characterization of a 230,000-dalton reticulocyte protein and peptides derived from it that affect the activity of a protein phosphatase.  Appendix.

Won Lee Paul W. Chun, Jong-zluk Kim, and Chan
Insulin action in denervated rat hemidiaphragms. Decreased hormonal stimulation of glycogen synthesis involves both glycogen synthase and glucose transport.

Myron L. Toews and John P. Perkins
Identification of mannose 6-phosphate receptors in rabbit alveolar macrophages.
Virginia L. Shepherd, Hudson H. Freeze, Arnold L. Miller, and Philip D. Stahl Correlation between cytosolic free Ca2+ and insulin release in an insulin-secreting cell line.
Characterization of the interchain and intrachain interactions between the binding sites of the free regulatory moiety of protein kinase I.

Stein Oue D0shlund and Dagfinn 0greid
In vivo studies of pyridine nucleotide metabolism in Escherichia coli and Saccharomyces cerevisiae by carbon-13 NMR spectroscopy. BC3H-1.

Pollet
Equilibrium model for insulin-induced receptor down-regulation. Regulation of insulin receptors in differentiated BC3H-1 myocytes.
Insulin regulation of insulin-like growth factor action in rat hepatoma cells.