Biochemical and Biophysical Research Communications
Isolation of novel rice (Oryza sativa L.) multiple stress responsive MAP kinase gene, OsMSRMK2, whose mRNA accumulates rapidly in response to environmental cues
Section snippets
Materials and methods
Plant material and in vitro system. Rice (O. sativa L. cv. Nipponbare) was grown under white fluorescent light (wavelength 390–500 nm, , 12 h light period/day) at 25 °C and 70% relative humidity as described previously [5], [6], [7]. For in vitro experiments, the middle portions of fully expanded leaves (approximately 2 cm long segments cut with sterile scissor) from two-week-old seedlings were used for all treatments under continuous light . Leaf segments placed in open
Identification of the OsMSRMK2 gene
To isolate rice genes involved in the early steps of rice plant defense/stress response pathways, we embarked on isolating specific genes induced in response to JA, a crucial endogenous signalling molecule [1], [2], [4], [5], [6], using differential cloning and identified a novel MAPK gene, OsMSRMK2 from japonica-type rice (cv. Nipponbare) seedling leaves. The OsMSRMK2 cDNA is 1356-bp long and contains an open reading frame of 1110 nucleotides capable of encoding a 369 amino acid polypeptide
Concluding remarks
Results described and discussions thereof clearly show that OsMSRMK2: (a) is a novel member of the MAPKs from japonica-type rice, (b) is induced by and thus perceives global signalling molecules and biotic and abiotic stresses on rice leaves and hence triggered in response to rice defense/stress cues, (c) is negatively regulated by a de novo synthesized protein factor, and very importantly (d) gives expression patterns at the transcript level that have not been seen (or shown) in dicots.
Acknowledgements
R.R. is a close collaborator of the RLABB and presently works at AIST supported by the Japan Society for Promotion of Science. We thank Drs. A. Kubo and H. Saji (National Institute of Environmental Studies, Tsukuba, Japan) for use of the ozone and sulfur dioxide fumigation facilities.
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These authors contributed equally to this work.