The human and mouse repertoire of the adhesion family of G-protein-coupled receptors☆
Section snippets
Results
Hidden Markov Models (HMM) for adhesion-GPCRs were created and used to search the National Center for Biotechnology Information (NCBI) human and mouse genome databases (http://www.ncbi.nlm.nih.gov) for new genes. BLASTP searches in the Celera genome database (http://www.celera.com) were also performed. The searches yielded 2 new human genes and 17 new mouse genes. The genes were subsequently assembled to the predicted full-length coding regions. All the sequences were confirmed to exist in both
Discussion
We identified 2 new human and 17 new mouse members of the adhesion family of GPCRs. The total number of human adhesion-GPCRs amounts to 33, while there seems to be 2 fewer adhesion-GPCRs in mouse. It is likely that this represents the entire adhesion gene repertoire in these species. However, it cannot be completely excluded that there exist additional genes that could have been missed due to inadequate sequencing or gene prediction. The study clearly shows a one-to-one relationship of the
Acknowledgements
These studies were supported by the Swedish Medical Research council, the Swedish Society for Medical Research, Svenska Läkaresällskapet, Petrus och Augusta Hedlunds Stiftelse, Magnus Bergvalls Stiftelse, Åke Wibergs Stiftelse, and Melacure Therapeutics AB, Uppsala.
References (22)
- et al.
LNB-TM7, a group of seven-transmembrane proteins related to family-B G-protein-coupled receptors
Trends Biochem. Sci.
(2000) - et al.
Prediction of complete gene structures in human genomic DNA
J. Mol. Biol.
(1997) - et al.
There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini
Biochem. Biophys. Res. Commun.
(2003) - et al.
Novel human G-protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions
FEBS Lett.
(2002) - et al.
ETL, a novel seven-transmembrane receptor that is developmentally regulated in the heart. ETL is a member of the secretin family and belongs to the epidermal growth factor-seven-transmembrane subfamily
J. Biol. Chem.
(2001) - et al.
alpha-Latrotoxin stimulated exocytosis by the interaction with a neuronal G-protein-coupled receptor
Neuron
(1997) - et al.
A latrophilin/CL-1-like GPS domain in polycystin-1
Curr. Biol.
(1999) - et al.
Long pentraxins: an emerging group of proteins with diverse functions
Cytokine Growth Factor Rev.
(1996) - et al.
Molecular tinkering of G-protein-coupled receptors: an evolutionary success
EMBO J.
(1999) - et al.
Drug design strategies for targeting G-protein-coupled receptors
Chembiochemistry
(2002)
The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints
Mol. Pharmacol.
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