Elsevier

Genomics

Volume 84, Issue 1, July 2004, Pages 23-33
Genomics

The human and mouse repertoire of the adhesion family of G-protein-coupled receptors

https://doi.org/10.1016/j.ygeno.2003.12.004Get rights and content

Abstract

The adhesion G-protein-coupled receptors (GPCRs) (also termed LN-7TM or EGF-7TM receptors) are membrane-bound proteins with long N-termini containing multiple domains. Here, 2 new human adhesion-GPCRs, termed GPR133 and GPR144, have been found by searches done in the human genome databases. Both GPR133 and GPR144 have a GPS domain in their N-termini, while GPR144 also has a pentraxin domain. The phylogenetic analyses of the 2 new human receptors show that they group together without close relationship to the other adhesion-GPCRs. In addition to the human genes, mouse orthologues to those 2 and 15 other mouse orthologues to human were identified (GPR110, GPR111, GPR112, GPR113, GPR114, GPR115, GPR116, GPR123, GPR124, GPR125, GPR126, GPR128, LEC1, LEC2, and LEC3). Currently the total number of human adhesion-GPCRs is 33. The mouse and human sequences show a clear one-to-one relationship, with the exception of EMR2 and EMR3, which do not seem to have orthologues in mouse. EST expression charts for the entire repertoire of adhesion-GPCRs in human and mouse were established. Over 1600 ESTs were found for these receptors, showing widespread distribution in both central and peripheral tissues. The expression patterns are highly variable between different receptors, indicating that they participate in a number of physiological processes.

Section snippets

Results

Hidden Markov Models (HMM) for adhesion-GPCRs were created and used to search the National Center for Biotechnology Information (NCBI) human and mouse genome databases (http://www.ncbi.nlm.nih.gov) for new genes. BLASTP searches in the Celera genome database (http://www.celera.com) were also performed. The searches yielded 2 new human genes and 17 new mouse genes. The genes were subsequently assembled to the predicted full-length coding regions. All the sequences were confirmed to exist in both

Discussion

We identified 2 new human and 17 new mouse members of the adhesion family of GPCRs. The total number of human adhesion-GPCRs amounts to 33, while there seems to be 2 fewer adhesion-GPCRs in mouse. It is likely that this represents the entire adhesion gene repertoire in these species. However, it cannot be completely excluded that there exist additional genes that could have been missed due to inadequate sequencing or gene prediction. The study clearly shows a one-to-one relationship of the

Acknowledgements

These studies were supported by the Swedish Medical Research council, the Swedish Society for Medical Research, Svenska Läkaresällskapet, Petrus och Augusta Hedlunds Stiftelse, Magnus Bergvalls Stiftelse, Åke Wibergs Stiftelse, and Melacure Therapeutics AB, Uppsala.

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  • Cited by (0)

    Supplementary material for this article may be found on ScienceDirect.

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