STAR Protocols
Volume 3, Issue 4, 16 December 2022, 101844
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Protocol
A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection

https://doi.org/10.1016/j.xpro.2022.101844Get rights and content
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Highlights

  • Protocol to identify a dual regulation of zebrafish LGP2 following viral infection

  • In vivo assays to compare fish mortality and IFN responses following viral infection

  • In vitro assays including dose titration and time titration in SVCV-infected fish cells

  • Applicable to a variety of fish cell types having IFN response to stimuli

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Summary

Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also describe in vitro assays including titration of infection duration in SVCV-infected fish cells to determine changes in IFN response. This protocol is effective to illuminate a regulatory switch of LGP2 in fish cells toward virus infection.

For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1

Subject areas

Immunology
Model Organisms
Molecular Biology
Signal Transduction
Protein Biochemistry

Data and code availability

This paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

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