STAR Protocols
Volume 1, Issue 3, 18 December 2020, 100175
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Protocol
Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis

https://doi.org/10.1016/j.xpro.2020.100175Get rights and content
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Highlights

  • Protocol for needle-induced wounding in C. elegans epidermis

  • Tips on how to use C. elegans epidermis for membrane repair

  • Diverse assays for measuring the consequence of membrane repair

Summary

Efficient membrane repair after injury is essential for cell and animal survival. Caenorhabditis elegans epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair in vivo. This protocol describes detailed approaches for how to perform wounding on the epidermis and how to examine membrane repair by trypan blue staining, confocal imaging, and data analysis.

For details on the use and execution of this protocol, please refer to Meng et al. (2020).

Subject Areas

Cell Biology
Cell Membrane
Model Organisms

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