Elsevier

Virology

Volume 407, Issue 2, 25 November 2010, Pages 185-195
Virology

Fragmentation of the Golgi apparatus provides replication membranes for human rhinovirus 1A

https://doi.org/10.1016/j.virol.2010.08.012Get rights and content
Under an Elsevier user license
open archive

Abstract

All viruses with a positive-stranded RNA genome replicate their genomic RNA in association with membranes from the host cell. Here we demonstrate a novel organelle source of replication membranes for human rhinovirus 1A (HRV-1A). HRV-1A infection induces fragmentation of the Golgi apparatus, and Golgi membranes are rearranged into vesicles of approximately 250–500 nm diameter. The newly distributed Golgi membranes co-localize with viral RNA replication templates, strongly suggesting that the observed vesicles are the sites of viral RNA replication. Expression of the HRV-1A 3A protein induces alterations in the Golgi staining pattern similar to those seen during viral infection, and expressed 3A localizes to the Golgi-derived membranes. Taken together, these data show that in HRV-1A infection, the 3A protein plays a role in fragmenting the Golgi complex and generating vesicles that are used as the site of viral RNA replication.

Keywords

Rhinovirus
Picornavirus
Golgi fragmentation
Replication membranes

Cited by (0)

1

Present address: School of Public Health, University of California, Berkeley, CA 94720, USA.