Elsevier

Ultrasonics Sonochemistry

Volume 77, September 2021, 105676
Ultrasonics Sonochemistry

Low-intensity ultrasound enhances the antimicrobial activity of neutral peptide TGH2 against Escherichia coli

https://doi.org/10.1016/j.ultsonch.2021.105676Get rights and content
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Highlights

  • A novel AMP TGH2 was screened from C-terminal sequence of T. granosa hemoglobin.

  • The MIC of TGH2 on E. coli decreased by 4-fold under ultrasound treatment.

  • The permeability on E. coli cell membrane changed with ultrasound treatment.

  • TGH2 had a slight untwisting effect under ultrasound treatment for 0.5 h.

Abstract

In recent years, foodborne diseases caused by Escherichia coli are a major threat to the food industry and consumers. Antimicrobial peptides (AMPs) and ultrasound both have good inhibitory effects on E. coli. In this work, the mechanism of action and synergistic effect of an in silico predicted AMP, designated as TGH2 (AEFLREKLGDKCTDRHV), from the C-terminal sequence of Tegillarca granosa hemoglobin, combined with low-intensity ultrasound was explored. The minimal inhibitory concentration (MIC) of TGH2 on E. coli decreased by 4-fold to 31.25 μg/mL under 0.3 W/cm2 ultrasound treatment, while the time kill curve analysis showed that low-intensity ultrasound combined with peptide TGH2 had an enhanced synergistic bactericidal effect after 0.5 h. The permeability on E. coli cell membrane increased progressively during combined treatment with peptide TGH2 and low-intensity ultrasound, resulting in the leakage of intracellular solutes, as shown by transmission electron microscopy (TEM). Structural analysis using circular dichroism (CD) revealed that peptide TGH2 has an α-helical structure, showing a slight untwisting effect under 0.3 W/cm2 ultrasound treatment for 0.5 h. The findings here provide new insight into the potential application of ultrasound and AMPs combination in food preservation.

Keywords

Antimicrobial peptide
Low-intensity ultrasound
Escherichia coli
Antimicrobial activity
TGH2

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