Experimental animal transplantationStem cells: LiverThe Phenotypic Characteristic of Liver-Derived Stem Cells From Adult Human Deceased Donor Liver
Section snippets
Isolation and Culture of HLSCs
This study was approved by our institutional review board, and all samples were obtained with informed consent. HLSCs were isolated from unused deceased donor whole liver that was not suitablet for liver transplantation. The donor was a 33 year-old female; the liver could not be transplanted because it was from a non–heart beating donor. Liver cell preparation was conducted using a slight modification of the two-step collagenase perfusion technique originally described by Seglen.4 In brief, the
Results
Morphologically, cultured HLSCs exhibited an elongated and spindled shape after 6 hours of seeding onto a culture flask (Fig 1). In an earlier study, fibroblastic features were described as a stem cell morphology.5 Characterization by flow cytometry revealed that the cells isolated by the described method were positive for stem cell markers CD29, CD73, CD44, CD90, CD105, and CD166, and negative for CD14 (monocyte), CD34 (heamatopoietic stem cell), and CD45 (leukocyte common antigen), indicating
Discussion
We identified in the adult human liver a cell population that fulfills the criteria for stem cell definition such as the capacity for self-renewal and multipotent differentiation. HLSCs expressed several mesenchymal but not hematopoietic stem cell markers and CD26 and CK18, indicating a partial commitment to hepatic cells. Isolation of a population of stem cells with similar biological potential from human liver tissue has been described earlier.2, 3, 6 HLSCs that were cultured contain distinct
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2015, Transplantation ProceedingsCitation Excerpt :Human liver tissue was obtained from a nontumor portion of the liver from a patient who received hepatic resection for hepatic hemangioendothelioma. Isolation of HLSCs was performed using a 2-step collagenase perfusion technique as previously described [3]. In the experiment, we used HLSCs from the 6th to 7th passages.
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Supported by a grant from the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A080917).