CRISPR/Cas systems accelerating the development of aptasensors
Graphical abstract
Section snippets
Introduction of CRISPR/Cas and aptamer
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nucleases proteins, termed as CRISPR/Cas systems [1,2], are the RNA-guided adaptive immunity mechanism formed in the long-term evolution of archaea and bacteria. Since its serendipitous discovery by Nakata's group in 1987 [1], scientists have been studying its function and mechanism and have gradually gained a relatively comprehensive understanding [[3], [4], [5]]. In August 2012, Jennifer Doudna and
The essential composition of Cas-aptasensors
CRISPR/Cas system is composed of palindromic sequences regions, spacer regions (exogenous DNA sequences), the upstream leader region corresponding to the promoter of the CRISPR sequence, a polymorphic family of genes (Cas genes) and Cas proteins [[5], [6], [7]]. Based on the different functions of Cas proteins and the mechanism of action, CRISPR/Cas system can be broadly classified into two categories: the Class 1 is a multi-protein effector system requiring 4–7 Cas protein subunits considered
CRISPR/Cas system-based aptasensors
CRISPR/Cas system has been rising since 2019 as the attractive stars in aptamers development with their superior properties. Based upon their methods of signal transduction, we classify these aptasensors into four major types of fluorescent, electrochemical, colorimetric, LERT, and other three types involving ICPMS, RRS, and SERS sensors. Besides, these Cas-aptasensors present many advantages such as rapid, sensitive, tunable, versatility, liquid biopsy and low cost, and provide miniaturized
Conclusion and future perspectives
To sum up, this review has summarized the recent advances in CRISPR/Cas system-enhanced aptasensors for the detection of a broad range of target analytes. Unique characteristics of CRISPR/Cas system have made them ideal for aptasensors fabrication, biological recognition and signal transduction. They have largely improved the sensitivity in bioanalysis coupled with some flexible technologies for yielding more acDNA, such as “locked-activated” mode, sandwich-type CLASA, “core-shell” nanoprobe
Declaration of competing interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgments
All authors made the contributions to the manuscript and had approved the final version of the script. We thank the support of the Natural Science Foundation of Shandong Province (no. ZR2022QB207), National Natural Science Foundation of China (no. 32102088, 21874010, 21827810), Supported by the Taishan Scholars Program, the Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2021B14, CXGC2022E05), the Young Elite Scientist Sponsorship
References (153)
The heroes of CRISPR
Cell
(2016)- et al.
Delivery of CRISPR-Cas tools for in vivo genome editing therapy: trends and challenges
J. Contr. Release
(2022) - et al.
Three new Cs for CRISPR: collateral, communicate, cooperate
Trends Genet.
(2019) - et al.
CRISPR-Cas12a: Functional overview and applications
Biomed. J.
(2020) - et al.
CRISPR-Cas12a-assisted nucleic acid detection
Cell Discov
(2018) - et al.
CRISPR/Cas-powered nanobiosensors for diagnostics
Biosens. Bioelectron.
(2022) - et al.
CRISPR-Cas systems: from gene scissors to programmable biosensors
TrAC-Trend. Anal. Chem.
(2021) - et al.
CRISPR/Cas systems towards next-generation biosensing
Trends Biotechnol.
(2019) - et al.
CRISPR-Cas based virus detection: recent advances and perspectives
Biosens. Bioelectron.
(2021) - et al.
Translation of aptamers toward clinical diagnosis and commercialization
Biosens. Bioelectron.
(2022)
Construction and bioapplications of aptamer-based dual recognition strategy
Biosens. Bioelectron.
Recent advances of aptasensors for exosomes detection
Biosens. Bioelectron.
CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
Chem. Sci.
Methodologies in visualizing the activation of CRISPR/Cas: the last mile in developing CRISPR-Based diagnostics and biosensing–A review
Anal. Chim. Acta
Advancing sensing technology with CRISPR: from the detection of nucleic acids to a broad range of analytes–A review
Anal. Chim. Acta
Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system
Cell
CRISPR-Cas12a based aptasensor for sensitive and selective ATP detection
Sensor. Actuator. B Chem.
Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics
Biosens. Bioelectron.
Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection
Biosens. Bioelectron.
“Aptamer-locker” DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis
Biosens. Bioelectron.
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
J. Biol. Chem.
Controlling the trans-cleavage of CRISPR-Cas12a with nicked PAM: universal platform for biosensing
Sensor. Actuator. B Chem.
ELISA Techniques, Methods in Enzymology
A CRISPR/Cas12a-assisted on-fibre immunosensor for ultrasensitive small protein detection in complex biological samples
Anal. Chim. Acta
Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins
Talanta
Gold nanoparticles as sensors in the colorimetric and fluorescence detection of chemical warfare agents
Coord. Chem. Rev.
Nano-immunosorbent assay based on Cas12a/crRNA for ultra-sensitive protein detection
Biosens. Bioelectron.
Aptamer-based cell-free detection system to detect target protein
Synth. Syst. Biotechnol.
A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes
Sci. Bull.
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection
Syn. Syst. Biotechno.
Accurate MRSA identification through dual-functional aptamer and CRISPR-Cas12a assisted rolling circle amplification
J. Microbiol. Methods
Hydrazone ligation assisted DNAzyme walking nanomachine coupled with CRISPR-Cas12a for lipopolysaccharide analysis
Anal. Chim. Acta
Emerging bioanalytical applications of DNA walkers
TrAC, Trends Anal. Chem.
An exceptional and universal DNA walker amplified “one-to-many” CRISPR/Cas12a-Mediated fluorescent biosensor for ultrasensitive detection of non-DNA biomarkers
Sensor. Actuator. B Chem.
CRISPR/Cas12a-based biosensors for ultrasensitive tobramycin detection with single-and double-stranded DNA activators
Sensor. Actuator. B Chem.
Accurate and sensitive analysis of Staphylococcus aureus through CRISPR-Cas12a based recycling signal amplification cascades for early diagnosis of skin and soft tissue infections
J.Microbiol. Meth.
Four-stage signal amplification for trace ATP detection using allosteric probe-conjugated strand displacement and CRISPR/Cpf1 trans-cleavage (ASD-Cpf1)
Sensor. Actuator. B Chem.
Interaction of single-and double-stranded DNA with multilayer MXene by fluorescence spectroscopy and molecular dynamics simulations
Chem. Sci.
A novel CRISPR/Cas14a system integrated with 2D porphyrin metal-organic framework for microcystin-LR determination through a homogeneous competitive reaction
J. Hazard Mater.
CRISPR/Cas12a-derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein
Biosens. Bioelectron.
CRISPR/Cas12a-based electrochemical biosensor for highly sensitive detection of cTnI
Bioelectrochemistry
Electrochemiluminescence covalent organic framework coupling with CRISPR/Cas12a-mediated biosensor for pesticide residue detection
Food Chem.
A highly sensitive electrochemical aptasensor for cocaine detection based on CRISPR-Cas12a and terminal deoxynucleotidyl transferase as signal amplifiers
Talanta
CRISPR-Cas12a-mediated label-free electrochemical aptamer-based sensor for SARS-CoV-2 antigen detection
Bioelectrochemistry
Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product
J. Bacteriol.
Evolution and classification of the CRISPR–Cas systems
Nat. Rev. Microbiol.
An updated evolutionary classification of CRISPR–Cas systems
Nat. Rev. Microbiol.
Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
Nat. Rev. Microbiol.
A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity
Science
Multiplex genome engineering using CRISPR/Cas systems
Science
Cited by (10)
The design strategies for CRISPR-based biosensing: Target recognition, signal conversion, and signal amplification
2024, Biosensors and BioelectronicsCRISPR/Cas12a linked sandwich aptamer assay for sensitive detection of thrombin
2024, Analytica Chimica ActaRecent and emerging trends of DNA/RNA hybridized COF nanoprobes for analytes detection and biomarkers imaging in clinical biomedicines
2024, TrAC - Trends in Analytical Chemistry