Role of metallomic strategies in developing ruthenium anticancer drugs
Graphical Abstract
While the development of metallodrugs remains an attractive area for refining metallomic techniques, all the supportive efforts of analysts have not yet been translated into the introduction of a new drug to market.
Introduction
As time goes by after the approval for world-wide clinical use of the latest of metal-based anticancer drugs, oxaliplatin (in 2002), the feeling is coming to the fore that there is something off beam in the state of metallodrug discovery and development. Taking into account over 40 years of intense research and spending that is difficult to assess numerically, even with cisplatin and carboplatin that formerly reached patients, ‘the three swallows do not make a summer.’ Yet more discouraging is the fact that in the meantime on average ten new organic drugs for oncology are introduced each year [1]. Certainly, a much bigger number of organic chemists in drug development as compared to metallodrug researchers can constitute justification but to what degree acceptable?
Lack of developmental productivity did not pass round ruthenium coordination and organometallic compounds, none of which has found its way to market. For instance, more than 25 years passed since the antitumor properties of (H2ind)[RuIIICl4(Hind)2] (I), perhaps the most potent Ru compound, were discovered. This is twice longer than it averagely requires for a successful medicine to be introduced to clinics. In this regard, it seems to be of immediate interest to discuss root causes of metallodrug development inefficiency and in particular, the challenges over the course of discovery and development for ruthenium-based would-be drugs. Given the scope of this journal and author's expertise, this review will rather highlight the state-of-the-art of analytical methodology used to identify, characterize and quantify metal species originating from the Ru drugs and to map their interactions with biomolecules in human body, as well as its potential role in improving the drug development process. Indeed, only such speciation information can mind the gaps in understanding the processes of drug delivery, uptake, and cell processing, including activation and targeting, at the molecular level. This comprehension has forced metallodrug developers to seek support of analytical chemists in order to revise the arsenal and design of techniques and methodologies in use. Their joint efforts over the last decade have resulted in adopting a variety of powerful, mostly mass spectrometry (MS)-based analytical tools from the armory of biospeciation analysis. This favorable situation has been reflected in recent review literature, including the most relevant to the subject of the present review in-depth surveys of metallomic and proteomic approaches to study the mode of action of anticancer metallodrugs by Messori and coworkers [2], Timerbaev and coauthors [3], Groessl and Hartinger [4], and Wang et al. [5]. Several more specialized review papers are available which provide coverage of contributions dealing with a specific method or a group of methods employed in the development of metal-based drugs [6], [7], [8], [9]. However, none of the metallomics-oriented reviews focuses on anticancer ruthenium compounds, albeit with respect to progress in their preclinical and early clinical examinations the topic is well covered [10], [11], [12], [13], [14], [15], [16]. Written by bioinorganic or medicinal chemists, these issues though often miss the point of ever-growing importance of analytical methodology in putting ruthenium drug candidates on the map of modern cancer chemotherapy and in general are of a little overoptimistic style.
Therefore, the aim of this review is to offer critical analysis of metallomic strategies that can promote the elucidation of the molecular mechanisms underlying the mode of action of anticancer or antimetastatic drug candidates based on ruthenium. Special emphasis is placed here on those lead compounds that are specified above (see the abstract; structural formulas are shown in Fig. 1). No consideration is thus given to other promising Ru agents regardless of how high their potential is valued by their developers. This is not only consistent with author's long-term research strategy, avoiding the use of rather expensive metallomic techniques in investigations of each compound in early stages of preclinical development (‘take not a musket to kill a butterfly!’). More broadly speaking, if a novel cytotoxic agent coming from an academic lab does not exhibit druglike properties and pass pharmacological screening tests, often competing with structurally similar compounds, how one could convince the analyst that there is another use of the data acquired than to publish another paper with a catchy title? This issue is particularly critical in the light of the significant resources being put into generating such data. Also, with regard to expertise of this journal readership, basics and instrumental aspects of metallomic methods are excluded from consideration but we cross-index the interested reader to review work which is mentioned above.
Section snippets
Current challenges in studying the mechanism of drug's action
There are several hypotheses suggested at the pioneering stages of introduction of ruthenium(III) anticancer drugs with the purpose to explain the mechanisms by which they are transported to cancer cells, activated therein, and commence the tumor-inhibiting action. The most important suggestions (see also an excellent critical appraisal by Bergamo and Sava [17]) are:
- (1)
the drug is delivered to the cell by transferrin (though not the main binding partner) and hence arrives to the cell interior
Transportation to cancer cell
Binding of the ruthenium drugs to plasma transport proteins has been of top concern in early metallomic studies and become well portrayed by using in particular capillary electrophoresis (CE) with on-line inductively coupled plasma (ICP)-MS detection, including real-plasma environment (see, e.g. [20], for an overview). (Note that the author gives the preference to the term ‘metallomics’ over ‘metalloproteomics,’ as the former one by definition encompasses a greater number of metal species,
Cellular activation
Judging mostly from the physiological accessibility of the Ru(III)/Ru(II) redox pair, it has long been suggested that the Ru drugs could be reduced in hypoxic tumor environment, giving rise to more labile and hence active Ru(II) species. However, it is still unclear what is responsible for the reduction, if any takes place. Perhaps the only evidence of that reduction does occur would be identification of the reduced ruthenium forms, preferably in the cytosol of cancer cells. With the objective
Subcellular distribution and targeting
The high sensitivity potential of ICP-MS combined with the easiness, with which different cell fractions can be obtained by using cell fractionation kits, facilitated the method's footing in metallodrug distribution studies [8], with no exception of ruthenium compounds under consideration [30], [31], [32]. However, in most related reports, including also those based on using other techniques, drug internalization was accomplished as its initial molecular entity. As follows from the above
Conclusions and prospects
Even though the present gaps in understanding the biological fate and effect of ruthenium-based anticancer drugs have not been closed over the last few years, there is no point to blame methodology itself. Applied in a complementary variety of integrated separation and detection arrangements, the metallomic techniques showed their value and irreplaceability by common biomedical tools to provide clues on possible mechanism of action. It also helps to remember that after all the intrinsic task of
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Ruthenium compounds as antitumor agents: New developments
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