Original communicationExpression and prognostic significance of prothymosin-α and ERp57 in human gastric cancer
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Patients and specimens
After Institutional Review Board approval, paraffin-embedded tissue from 131 patients who had gastric adenocarcinoma resected at Johns Hopkins University Hospital between 1985 and 1995 was evaluated retrospectively. The archived tissue from these patients had been constructed previously into 7 TMA blocks by the Johns Hopkins University Department of Pathology. The TMAs contained 3 to 8 tissue samples from each patient, for a total of 663 cores. Each sample consisted of a 1.5-mm diameter core
Increased expression of prothymosin-α in gastric adenocarcinoma lesions
We evaluated prothymosin-α expression in gastric samples by staining the seven tissue arrays constructed at Johns Hopkins with a monoclonal antibody against prothymosin-α and horseradish peroxidase histochemistry. Normal gastric mucosa samples showed the strongest nuclear prothymosin-α staining predominantly in cells in the gland neck (epithelial progenitor zone) and in surface mucous cells. Cells of the deep gland including chief and parietal cells were generally negative (Fig 1A). In
Discussion
Gastric cancer is a major cause of worldwide cancer mortality, yet the factors influencing the biologic behavior of this tumor remain poorly understood. Discovery of new biomarkers for this disease may yield important insights into the biology of this disease and could prove useful for both early detection and the development of new therapies. Studies in mouse models have yielded several potential biomarkers for gastric preneoplasia and neoplasia,11, 13 however their role in human disease has
References (31)
- et al.
Mice lacking secretory phospholipase A2 show altered apoptosis and differentiation with Helicobacter felis infection
Gastroenterology
(1998) - et al.
Association of spasmolytic polypeptide expressing metaplasia (SPEM) with carcinogen administration and oxyntic atrophy in rats
Lab Invest
(2002) - et al.
Expression of cytoplasmic TFF2 is a marker of tumor metastasis and negative prognostic factor in gastric cancer
Lab Invest
(2003) - et al.
Relationship of spasmolytic polypeptide expressing metaplasia (SPEM) to pre-neoplasia in H. felis-infected mice
Gastroenterology
(2004) - et al.
Prothymosin alpha functions as a cellular oncoprotein by inducing transformation of rodent fibroblasts in vitro
J Biol Chem
(2001) - et al.
Tissue concentrations of prothymosin alpha: a novel proliferation index of primary breast cancer
Eur J Cancer
(1993) - et al.
Down-regulation of HLA class I antigen processing molecules: an immune escape mechanism of renal cell carcinoma?
J Urol
(2004) - et al.
HLA class I antigen abnormalities and immune escape by malignant cells
Semin Cancer Biol
(2002) - et al.
Patterns of gastric atrophy in intestinal type gastric carcinoma
Cancer
(2002) A human model of gastric carcinogenesis
Cancer Res
(1988)
Role of Helicobacter pylori gastritis in gastric atrophy, intestinal metaplasia and gastric neoplasia
Microsc Res Tech
Intestinal metaplasia types and the risk of gastric cancer: a cohort study in Slovenia
Int J Cancer
Exploring the link between Helicobacter pylori and gastric cancer
Aliment Pharmacol Ther
Identification of a metaplastic cell lineage associated with human gastric adenocarcinoma
Lab Invest
Spasmolytic polypeptide expressing metaplasia (SPEM) associated with gastric cancer in Iceland
Dig Dis Sci
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Supported by grants from Department of Veterans Affairs Merit Review Award, Vanderbilt SPORE in Gastrointestinal Cancer (1P 50 CA95103), the AGA Funderburg Award in Gastric Biology Related to Cancer, Discovery Grant from the Vanderbilt-Ingram Cancer Center, a Vanderbilt Clinical Oncology Research Career Development Program Fellowship (K12 CA090625), CA67108 from the National Cancer Institute, and Johns Hopkins SPORE in Gastrointestinal Cancer (P50 CA62924) from NCI.