Short communicationUsing lipid analysis and hyphal length to quantify AM and saprotrophic fungal abundance along a soil chronosequence
Section snippets
Conclusions
While microscopic measurement of hyphal length has been the most widely used method for estimating AM biomass in soil, the method does not allow systematic or functional separation of different (e.g. AMF versus SF) fungal mycelia, nor reliable separation of the dead and live fraction of fungal biomass (Sylvia, 1992, Olsson, 1999). In contrast, lipid biomarkers allow for sensitive and simultaneous estimation of live AMF and SF biomass. Overall, we found that phospholipids 16:1ω5c and 18:2ω6,9c
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