Regulation of CyR61 expression and release by 3-mercaptopyruvate sulfurtransferase in colon cancer cells

Cysteine-rich angiogenic inducer 61 (CYR61, also termed CCN family member 1 or CCN1), is a matricellular protein encoded by the CYR61 gene. This protein has been implicated in the regulation of various cancer-associated processes including tumor growth, angiogenesis, tumor cell adhesion, migration, and invasion as well as the regulation of anticancer drug resistance. Hydrogen sulfide (H2S) is a gaseous endogenous biological mediator, involved in the regulation of cellular bioenergetics, angiogenesis, invasion, and chemotherapeutic resistance in several types of cancer. H2S is produced by three enzymes: cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST). The current studies were set up to investigate if CBS or 3-MST regulates CyR61 in colon cancer cells in the context of the regulation of proliferation, migration, and survival. The study mainly utilized HCT116 cells, in which two of the principal H2S-producing enzymes, CBS and 3-MST, are highly expressed. The H2S donor GYY4137 and the polysulfide donor Na2S3 activated the CyR61 promoter in a concentration-dependent fashion. Aminooxyacetic acid (AOAA), a pharmacological inhibitor of CBS as well as HMPSNE: 2-[(4-hydroxy-6- methylpyrimidin-2-yl)sulfanyl]-1-(naphthalen-1-yl)ethan-1-one, a pharmacological inhibitor of 3-MST inhibited CyR61 mRNA expression. This effect was more pronounced in response to HMPSNE than to AOAA and occurred through the modulation of S1PR via ATF1 and CREB. CyR61 was found to play an active, but relatively minor role in maintaining colon cell proliferation. HMPSNE markedly suppressed the secretion/release of CyR61 from the colon cancer cells. Moreover, HMPSNE promoted colon cancer cell apoptosis; endogenously produced CyR61 was found to counteract this effect, at least in part via RhoA activation. Taken together, we conclude that the upregulation of 3-MST in cancer cells exerts cytoprotective effects and confers the cancer cells a more aggressive phenotype – at least in part via the modulation of CyR61 expression and release.

The Wnt/β-catenin pathway plays a fundamental role in cancer development and metastasis [50,51]. Several studies have demonstrated that this pathway is both interlinked with CyR61 and with various H 2 S-associated pathways [20,[52][53][54]

H 2 S and polysulfides activate the CyR61 promoter.
To examine the effect of H 2 S and polysulfides on the activity of the CyR61 promoter, we have introduced a viral vector containing a CyR61 promoter with an inducible mCherry reporter into HCT116 cells. HCT116 is a human colon cancer cell line, in which the pathogenetic role of CBS and 3-MST has previously been demonstrated [4,[9][10][11]20]. The slow-release H 2 S generator GYY4137, as well as the polysulfide donor Na 2 S 3 concentration-dependently activated the CyR61 promoter (Fig. 1A,B,C). In line with the bell-shaped concentration-response character of H 2 Swhere low and intermediate concentrations of this mediator serve regulatory roles, while higher concentrations become inhibitory, at least in part via inhibition of mitochondrial Complex IV [1] -a slight cytotoxic effect was observed with the highest GYY4137 and Na 2 S 3 concentrations used (Fig. 1D). However significant CyR61 promoter activation was already noted at lower concentrations of GYY4137 (1-3 mM) and Na 2 S 3 (100 μM), where these agents exerted no adverse effects on cell viability ( Fig. 1A,B,C).
Inhibition of 3-MST suppresses CyR61 mRNA expression through S1PR via ATF1 and CREB. Since our data indicated that H 2 S and polysulfides are able to induce the activation of the CyR61 promoter, we next examined the effect of H 2 S biosynthesis inhibition on CyR61 mRNA expression in HCT116 cells. Cells were treated with various concentrations of prototypical H 2 S biosynthesis inhibitors for 48 h. We used HMPSNE for inhibition of 3-MST; and AOAA for inhibition of CBS. We have also tested the combined application of both agents since in some cases after inhibition of one biological source of H 2 S, cellular responses can be partially compensated by another H 2 S producing enzymes [1,15]. Using live cell imaging, we quantified H 2 S production in HCT116 cells after treatment of the cells with HMSPNE or AOAA. There was a significant concentration-dependent decrease of H 2 S levels in response to both inhibitors (Fig. 2). We have also quantified CyR61 mRNA levels after treatment of the cells with HMSPNE or AOAA and found that each inhibitor, as well as the combination of these two inhibitors significantly decreased CyR61 mRNA levels (Fig. 3A).
Next, we have conducted a series of experiments to investigate the mechanism involved in the above effect. Previous studies have demonstrated that CyR61 expression can be upregulated via S1PR (sphingosine 1-phosphate receptor), RhoA GTPase and p38MAPK signaling [45,48]. Thus, we have investigated the expression of S1PR (sphingosine 1-phosphate receptor), CREB and ATF1 (p38MAPK signaling pathway) in the current experimental system and tested the effect of H 2 S biosynthesis inhibition. Although HMPSNE had no significant effect on S1PR2 mRNA, a significant downregulation of S1PR2 protein level was observed after treatment of the cells with the 3-MST inhibitor. In contrast, the CBS inhibitor AOAA significantly increased S1PR2 mRNA and protein levels. When the two inhibitors were tested in combination, a significant decrease of S1PR2 protein level was noted (Fig. 3A,B,C). ATF1 protein was significantly downregulated in response to either of the two inhibitors as well as in response to their combination, although the inhibitory effect of these agents on its mRNA was relatively slight (Fig. 3A,B,C). Similarly to S1PR2, CREB protein was also downregulated by treatment of the cells with the 3-MST inhibitor HMPSNE, while AOAA did not exert an inhibitory effect at high concentrations, and it exerted stimulatory effects at lower concentrations ( Fig. 3B and C).  3-MST. β-catenin expression, or the expression of ACLY protein (which is involved in the regulation of the Wnt/β-catenin pathway) were also unaffected by CyR61 silencing (Fig. 4A and B).
CyR61 silencing attenuates colon cancer cell proliferation, but does not affect migration. Next, we have examined if the CyR61 silencing modulates HCT116 migration or proliferation. We have selected the HCT116 Sh3 CyR61 cell line with the strongest CyR61 downregulation and performed an IncuCyte Scratch Wound Assay and an IncuCyte Cell Count Proliferation Assay. CyR61 silencing produced a slight, but statistically significant suppression of cell proliferation. However, it did not affect cell migration (Fig. 5A,B,C).
Cyclin D1 downregulation in part via ATF1, CREB and CDK4 promotes cell cycle arrest in G1-S phase, leading to apoptosis and necrosis. The transcription factors AP-1, composed by ATF, JUN and inhibition of S1PR via ATF1 and CREB (p38MAPK pathway). A: Quantitative real-time PCR (qPCR) analysis of endogenous mRNA levels of CyR61, S1PR2 and ATF1 in HCT116 cells after incubation with HMPSNE, AOAA or their combination for 48 h. Data are shown as mean ± SEM of at least 4 independent experiments, *p < 0.05, **p < 0.01 compared to control. B,C: Western blot analysis of S1PR2 and p38MAPK pathway markers in presence of HMPSNE, AOAA or their combination for 48 h. Data are shown as mean ± SEM, n = 4, **p<0.01 compared to control.
FOS, and CREB can activate the Cyclin D1 promoter, a key player in G1-S phase transition [47,[55][56][57]. Since 3-MST inhibition suppresses ATF1 and CREB protein expression (Fig. 3A,B,C), we have examined if HMPSNE also affects cyclin D1 expression. HMPSNEalone or combined with AOAAcaused a significant downregulation of cyclin D1, when both agents were used at their highest concentrations ( Fig. 6A and B). This downregulation can be explained in part by a decrease in ATF1 and CREB expression. However, it does not explain why Cyclin D1 is downregulated at lower HMPSNE concentrations, where the expression of ATF1 or CREB is not decreased. This finding indicates that others   signalling processes may also contribute to the regulation of Cyclin D1 in the current experimental system. Thus, we next measured the expression of two other molecules, CDK4 and GSK-3β -both of them being known regulators of Cyclin D1 activation [55][56][57][58]. Indeed, at high concentrations of HMPSNE, CDK4 was found to be downregulated and therefore may be potentially involved in the regulation of Cyclin D1.
When Cyclin D1 is downregulated, cell cycle arrest is known to occur [59][60][61], which, in turn, can induce apoptotic cell death [62]. To examine if HMPSNE promotes apoptosis and/or necrosis in HCT116 cells, we used the APC Annexin V Apoptosis Detection Kit with Propidium Iodide Solution. This assay can discriminate cells in early apoptosis (annexin-positive and PI-negative cells), late apoptosis (annexin-and PI-positive cells) and necrosis (annexin-negative and PI-positive cells). Our results show that HMPSNE, at the higher concentrations used, leads to a significant increase in the apoptotic cell population, mainly representing the late form of apoptosis; it also increases the necrotic cell population ( Fig. 6C and D). It is important to notice that, at lower HMPSNE concentration (30 μM) despite a significant downregulation of Cyclin D1, the activation of apoptosis was not statistically significantalthough a trend for an increase was already noted. At this concentration of the inhibitor, CREB, CDK4 and GSK-3 were found to be upregulated. Thus, when 3-MST activity is only partially inhibited, the effects on various signalling pathways may be variable; the effects may be functionally diverse, which, ultimately, only exerts a slight net effect on the process of apoptosis.
In order to extend our findings to other colon cancer cells, we have also tested the effect of the 3-MST inhibitor on HT-29 and LoVo cells, two additional human colon cancer cell lines which are known to produce H 2 S from CBS and 3-MST. HMPNSE caused the downregulation of cyclin D1, most likely via downregulation of ATF1 in these cells ( Fig. 7A and B) and increased the apoptotic and necrotic cell populations ( Fig. 8A and B). In HCT116 cells, HMPSNE tended to induce more apoptosis than necrosis, while in LoVo and HT-29 cells it induced more necrosis and less apoptosis ( Fig. 6C and D and Fig. 8A and B).
3-MST inhibition induces BID downregulation and CyR61 upregulation in part via RhoA, thereby modulating apoptosis. Since we have observed that HMPSNE or AOAA decrease CyR61 mRNA expression (Fig. 3A), we expected that a consequent downregulation of the CyR61 protein would also occur. However, HMPSNE and AOAA significantly increased CyR61 proteins levels ( Fig. 9A and B). We have, in turn, investigated the mechanism underlying this unexpected response. Our results show that HMPSNE (but not AOAA) increased RhoA protein level ( Fig. 9A and B). Since this protein is known to be involved in the stimulation of CyR61 expression [45,63], we hypothesize that RhoA expression may be an additional effect of 3-MST inhibition, which, in turn, counterbalances the suppression of CyR61 mRNA levels. The reduction of CyR61 mRNA is more pronounced with HMPSNE than with AOAA; this difference may explain why RhoA expression in response to AOAA is upregulated to a smaller degree than in response to HMPSNE (Fig. 3A). It is important to mention that RhoA can only partially explain the upregulation of CyR61. At lower concentrations of HMPSNE (100 μM) RhoA appears to be the principal player in CyR61 upregulation, but its contribution to the decrease in CyR61 secretion appears to be slighter. However, at higher HMPSNE concentrations, the slight upregulation of RhoA cannot explain the marked upregulation of CyR61: in this case we observed a marked blockade of CyR61 secretion, which, in turn, is reflected in a marked accumulation of CyR61 inside the cell (Fig. 10).
Next, we have tested the hypothesis that the upregulation of CyR61 in colon cancer cells serves to prevent apoptosis. When HCT116 ShCTR and HCT116 Sh3 CyR61 cells were treated with HMPSNE for 48 h, the number of apoptotic cells was found to be higher in the CyR61 silencing cell line than in the wild-type control cells, suggesting that CyR61 may serve to prevent or delay apoptosis ( Fig. 9C and D). RhoA upregulation and BID downregulation were also observed after HMPSNE treatment ( Fig. 9A and B); we hypothesize that these effects may also participate in the development of apoptosis resistance, consistent with such function of these proteins in other cell types [64,65]. Contrary to HCT116 cells, 300 μM of HMPSNE significantly decreased CyR61 proteins levels in HT-29 and LoVo cells ( Fig. 7A and B), highlighting a colon cancer cell-type-dependence of the regulation of CyR61 by the 3-MST system.

Pharmacological inhibition of H 2 S biosynthesis suppresses Snail and Caveolin-1 expression, downregulates Shh and inhibits CyR61
secretion. CyR61 is present in two forms: cell-associated as well as a secreted/circulating form, with the latter being considered a soluble, circulating mediator, as well as a biomarker [26][27][28][29][30]. In order to investigate if pharmacological inhibition of 3-MST or CBS affects the secretion/release of CyR61 from HCT116 cells, we incubated HCT116 cells with various concentrations of HMPSNE or AOAA (either alone, or in combination) for 48 h and then performed an ELISA to quantify soluble CyR61 levels in the cell culture supernatant. HMPSNE and AOAA both exerted a marked suppressive effect on the secretion of CyR61 in HCT116 cells (Fig. 10A). Similar effect was also observed in HT-29 and LoVo cells (Fig. 7C).
To assess which molecules could be involved in the HMPSNE-or AOAA-induced inhibition of Cyr61 release, we have measured Snail and Caveolin-1 expression, two molecules that are known to be involved in the promotion of CyR61 secretion from various cancer cells [28,66]. Fig. 10B and C shows that both of these proteins are downregulated after treatment of the cells with HMPSNE and to a smaller extent after treatment of the cells with AOAA. HMPSNE also resulted in the inhibition of the expression of Sonic Hedgehog (Shh) protein ( Fig. 10B and C). This proteinan endogenous ligand of the hedgehog pathwayhas also been implicated previously in the regulation of CyR61 in various forms of cancer [67,68].

Discussion
In separate sets of studies, the significant pathophysiological role of (a) the upregulation of various H 2 S-producing enzymes and (b) CyR61 biosynthesis and secretion has been already defined in various forms of cancer, including colon cancer (see Introduction). However, the potential relationship between H 2 S and the CyR61 pathway has not yet been examined, and the current project was designed to examine a potential connection between them. The fact that in colorectal cancer, on one hand, H 2 S and polysulfides are overproduced, and on another hand, CyR61 is overexpressed, led us to speculate that increased H 2 S levels, due to the induction of various H 2 S-producing enzymes in cancer cells, may induce or activate CyR61, which, in turn, may contribute to cancer aggressiveness. The results of the current report show that H 2 S and polysulfides can, indeed, activate the CyR61 promoter (Fig. 1A,B,C), and HMPSNE and AOAA suppress CyR61 mRNA levels (Fig. 3A) in various colon cancer cell types. However, the data also demonstrated that this activation does not always or necessarily yield higher CyR61 intracellular protein levels, due to a combination of reasons (see below).
What, then, is the molecular mechanism of CyR61 promoter activation by 3-MST? We do not currently know if this activation reflects H 2 S/ polysulfide overproduction, followed by a direct biding of these reactive species to promoter elements, or if this effect is due to a possible activation or modification of regulators that bind to the CyR61 promoter. An important global regulatory mechanism triggered by H 2 S/polysulfides is S-sulfhydration (also termed persulfidation), a fundamental posttranslational mechanism which can affect hundreds or thousands of proteins in various mammalian cells [1,[69][70][71][72]. Although S-sulfhydration was originally linked to H 2 S, it is now well accepted that this modification is principally mediated by polysulfide chemistry (and not by H 2 S or HS − ). When a H 2 S donor (like the slow-acting donor GYY4137 in our experiments) is applied to a cell, nevertheless, it will generate a mixture of polysulfides and H 2 S [73,74]. The fact that in the current experiments both H 2 S and the polysulfide donor (Na 3 S 2 ) induced concentration-dependent inductions of the CyR61 promoter suggests that a sulfhydration mechanism may be involved. Indeed, prior studies have demonstrated that various promoters can be regulated by sulfhydration; for instance the Nrf2/ARE pathway is regulated by sulfhydration of Keap1 [75]. Various elements of the NF-κB pathway (IKKβ, as well NF-κB itself) have also been shown to be subject to sulfhydration [76,77]. Moreover, Sp1 -a known activator of the CyR61 promoterwas also found to be subject of S-sulfhydration, which, in turn, was shown to regulate mRNA expression [78][79][80]. Thus, our current working hypothesis is that endogenously generated H 2 S (most likely via intermediary polysulfides) induces CyR61 mRNA through Sp1 sulfhydration in colon cancer cells.
In order to determine if CyR61 levels are, indeed, regulated by endogenously produced H 2 S, we have applied AOAA and HMPSNE. These compounds are standard pharmacological inhibitors of the principal H 2 S-producing enzymes CBS and 3-MST, respectively. We have selected appropriate concentrations of these agents, where these compounds produce initially a partial, and then a complete inhibition of H 2 S synthesis from their respective enzymatic sources [20]. Both inhibitors produced a significant inhibition of CyR61 mRNA levels (Fig. 3A), indicating the importance of endogenous H 2 S/polysulfides in the upregulation of the CyR61 promoter in colon cancer cells.
Since the CyR61 promoter has been shown to be activated by S1P through RhoA GTPase and p38MAPK signaling pathways [45][46][47][48][49], we have also quantified the effect of H 2 S biosynthesis inhibition on the expression of S1PR, ATF1 and CREB. Our results show that these molecules are also downregulated by HMPSNE (Fig. 3A,B,C). Thus, we conclude that endogenous H 2 S/polysulfide biosynthesis by 3-MST in colon cancer cells promotes CyR61 mRNA induction, most likely through Sp1 sulfhydration, and through the activation of S1PR, ATF1 and CREB. The finding that the 3-MST inhibitor exerted more pronounced effects than the CBS inhibitor is consistent with the fact that 3-MST is the more significant biological source of polysulfides while CBS predominantly generates "free" H 2 S [1,81].
While the H 2 S system regulates the activation of the CyR61 promoter, CyR61 does not appear to regulate the expression of the two principal H 2 S-producing enzymes in HCT116 cells (Fig. 4A and B).
Previous studies have demonstrated that CyR61 upregulation can activate the Wnt/β-catenin pathway in gliomas [52] and that β-catenin activation or overexpression can upregulate CyR61 in hepatocellular carcinomas and pancreatic cancers [53,54]. However, the influence of CyR61 in Wnt/β-catenin activation in colon cancer has not been examined previously. Our results show that CyR61 does not promote the activation of the Wnt/β-catenin pathway activation in colon cancer cells ( Fig. 4A and B). However, the partial nature of the CyR61 silencing achieved (60% downregulation) must be taken into consideration when interpreting these results; it is possible that a more complete downregulation or complete deletion of CyR61 may have produced a different effect.
CyR61 overexpression in colon cancer cells leads to increased cell migration, invasion and high CyR61 in cancer patients and is associated with poor clinical prognosis [26,[40][41][42][43][44]. In our experiments, partial CyR61 silencing had no significant impact on HCT116 migration and only exerted a very slight inhibitory effect on cell proliferation (Fig. 5A, B,C). Once again it is possible that the remaining 40% of CyR61 after CyR61 silencing may be sufficient to maintain the functionality of the CyR61 system in regulating proliferation and migration. Alternatively, there may be a compensatory upregulation of other effector pathways that may compensate after CyR61 silencing and maintain functionality of these cells. Further studies, e.g. using a cell line with a complete deletion of this protein, would be needed to further explore the role of CyR61 in the regulation of HCT116 proliferation and migration.
The HMPSNE-induced downregulation of ATF1 and CREB (Fig. 3A,B, C) coincided with a decrease in Cyclin D1 protein expression (Fig. 6A  and B). We hypothesized that this event may promote a cell cycle arrest in G1/S phase. Masamha and Benbrook have previously observed that Cyclin D1 degradation is sufficient to induce G1 cell cycle in ovarian cancer cells [58]. Radu and colleagues have reported that the downregulation of Cyclin D1 induced by PTEN promotes cell G1 cycle arrest in glioblastomas [59]. In addition, O'Connor et al. demonstrated that poly(ADP-ribose) polymerase family member 14 downregulation decreases Cyclin D1 and promotes G1 cell cycle arrest [60]. Since

Fig. 8. 3-MST inhibition promotes apoptosis and necrosis in HT-29 and LoVo cells. A: Representative FACS plots showing the Q1 population (early apoptotic cells), Q2 population (late apoptotic cells), Q3 population (necrotic cells) and Q4 population (cells alive). B:
Quantification of apoptotic cells, distinguishing the late and the earlier apoptosis ratios, and necrotic cells, compared to control. Data are shown as mean ± SEM, n = 5, **p<0.01 compared to control.
prolonged cell cycle arrest can culminate in cell death [61] we have investigated the effect of 3-MST inhibition on the apoptotic/necrotic state of the HCT116 cells (as well as a number of additional human colon cancer cell lines). Our results confirm that the Cyclin D1 downregulation increases late apoptosis and necrosis, with the relative extent of the two forms of cell death being cell-type dependent (Fig. 6C, 7A,B,8A,B).
Moreover, our data ( Fig. 9C and D) suggest that CyR61 may play a role in preventing or retarding the apoptosis in response to 3-MST inhibition. This conclusion is based on the finding that CyR61 silencing in HCT116 cells increases the portion of apoptotic cells in response to HMPSNE incubation.
Importantly, 3-MST inhibition decreased CyR61 mRNA levels, but did not always suppress cell-associated CyR61 protein levels; in HCT116, in fact, a paradoxical increase was noted ( Fig. 9A and B) -while in HT-29 and LoVo cells a decrease was observed ( Fig. 7A and B). Upregulation of cell-associated CyR61 in HCT116 cells may be explained on one hand by the increased expression of RhoA proteins ( Fig. 9A and B), known to be involved in the stimulation of CyR61 expression [45,62], which may protect the CyR61 mRNA and the protein from degradation, i.e. prolongs their half-life. On the other hand, the increase of cell-associated CyR61 protein levels after HMPSNE treatment in HCT116 cells may also be related to the fact that 3-MST inhibition blocks the secretion/release of CyR61 into the cell culture supernatant (Fig. 10A), thereby retaining more protein in the cell-associated component. We speculate that such an effect may be therapeutically beneficial, since circulating CyR61 protein may exert pro-angiogenic, pro-inflammatory and immune-cell-modulatory effects [82][83][84][85][86] that are likely tumor-supportive in their nature. As already mentioned above, the current study demonstrates that the H 2 S/CyR61 interactions are not only (likely) different in different forms of cancer, but even are heterogeneous when comparing different human colon cancer cell lines. Contrary to HCT116 cells, HMPSNE significantly reduced cell-associated CyR61 protein levels in HT-29 and LoVo cells ( Fig. 7A and B). This difference may explain the different ratios apoptotic/necrotic cells observed between HCT116 versus HT-29 and LoVo cells, HMPSNE promoting mainly apoptosis in HCT116 cells and mainly inducing necrosis in HT-29 and LoVo cells. Besides CyR61, RhoA upregulation and BID downregulation after 3-MST inhibition (Fig. 9A and B), may also contribute to the development of apoptosis resistance. Indeed, in prior studies Zhang and Jiang described that RhoA inhibits the hypoxia-induced apoptosis in chondrocytes [63] and Khaider and colleagues demonstrated that the inhibition of BID expression by Akt leads to resistance to TRAIL-induced apoptosis in ovarian cancer cells [64]. Our findings, demonstrating that CyR61 in HCT116 cells plays an important role in cell survival after induction of apoptosis, are conceptually in line with prior studies concluding that CyR61 confers cytoprotective effectsincluding chemotherapy resistancein various forms of advanced cancers including colorectal [87][88][89][90].
The inhibitory effect of H 2 S biosynthesis inhibition on CyR61 secretion into the supernatant was very prominent, and this response was consistent across all human colon cancer cell lines studied (Figs. 7C and 10A). Snail and Caveolin-1 are known to be involved in CyR61 secretion [28,65]; both of these proteins were found to be downregulated with HMPSNE or by, AOAA (Fig. 10B and C). The suggestion that Snail downregulation is involved in the suppression of Cyr61 secretion is consistent with the results of Tanaka and colleagues who demonstrated that Snail promotes CyR61 secretion in squamous cell carcinoma [28]. In our study, we have also detected a decrease of Shh protein after inhibition of 3-MST in HCT116 cells ( Fig. 10B and C). This protein, as a ligand of the sonic hedgehog pathwaysimilar to CyR61 and Caveolin-1 -is also involved in the stimulation of cancer cell proliferation and migration [91][92][93]. Downregulation of these molecules may contribute to the previously reported [20] suppression of HCT116 cells migration after pharmacological inhibition of H 2 S biosynthesis.

Conclusions and implications
The main conclusions of the current study can be integrated into the working hypothesis outlined in Fig. 11. According to this hypothesis, (1) endogenous, 3-MST-derived H 2 S and H 2 Sn biosynthesis in cancer cells induces Sp1 sulfhydration, S1PR activation and induces the p38MAPK signaling pathway through ATF1 and CREB activation. (2) The reduction of H 2 S and H 2 Sn biosynthesis, in response to pharmacological inhibition of 3-MST, suppresses this pathway. (3) The reduction of ATF1 mRNA and protein levels after inhibition of H 2 S and H 2 Sn biosynthesis through 3-MST inhibition leads to cyclin D1 downregulation, promoting cell cycle arrest and inducing apoptosis. (4) Although inhibition of H 2 S and H 2 Sn biosynthesis through 3-MST inhibition suppresses CyR61 mRNA, this effect is not always reflected in a decrease in cell-associated CyR61 protein levels, which show a heterogeneous response (an increase in HCT116 cells and a decrease in HT-29 and LoVo cells). This is likely due to the combination of two effects: (5) After 3-MST inhibition, there is an increase of RhoA proteins in the cells, which can stabilize CyR61 mRNA and protein and (6) 3-MST inhibition produces a blockade of CyR61 secretion, thereby retaining some of this protein in the cellassociated fraction. (7) The latter effect is likely related to the suppression of Snail, Caveolin-1 and Shh proteins, which all have a known role in the stimulation of CyR61 secretion in cancer cells. Finally, (8) when CyR61 is retained in the cell, it plays a role as an anti-apoptotic factor.
In conclusion, the current study, by unveiling the regulation of CyR61, tumor-derived H 2 S and H 2 Sn produced by 3-MST, identified an additional pathway through which cancer cells utilize H 2 S and H 2 Sn to their benefit. This mechanism adds to the list of mechanisms through which pharmacological inhibition of cancer cell H 2 S and H 2 Sn biosynthesis may exert antitumor effects.   11. Modulation of the CyR61 pathway by endogenously produced 3-MST related H 2 S/polysulfides. Modulation of the CyR61 pathway by endogenously produced H 2 S/polysulfides: a working hypothesis. A: Endogenous, 3-MST-derived H 2 S and H 2 Sn biosynthesis in cancer cells induces Sp1 sulfhydration, S1PR activation and induces the p38MAPK signaling pathway through ATF1 and CREB activation, leading to increased levels of CyR61 mRNA and CyR61 protein. B: The reduction of H 2 S and H 2 Sn biosynthesis, achieved by a pharmacological 3-MST inhibitor, suppresses this pathway. The reduction of ATF1 mRNA and protein levels after inhibition of 3-MST inhibition leads to Cyclin D1 downregulation, promoting cell cycle arrest and inducing apoptosis. Although inhibition of H 2 S and H 2 Sn biosynthesis suppresses CyR61 mRNA, this effect is not always reflected in a decrease in cell-associated CyR61 protein levels, which show a heterogeneous response (an increase in HCT116 cells and a decrease in HT-29 and LoVo cells). This is likely due to the combination of two effects: (a) after 3-MST inhibition, there is an increase of RhoA proteins in the cells, which can stabilize CyR61 mRNA and protein and (b) 3-MST inhibition produces a blockade of CyR61 secretion, thereby retaining some of this protein in the cell-associated fraction. The latter effect is likely related to the suppression of Snail and Caveolin-1, which have a known role in the stimulation of CyR61 secretion in cancer cells. The decrease of Caveolin-1, CyR61 secretion and Shh, contribute to the decreased HCT116 migration demonstrated in our previous study [20]. Finally, when CyR61 is retained in the cell, it plays a role as an anti-apoptotic factor.
Western blotting. The cells were scraped from the well plate surface and lysed with RIPA lysis buffer (Thermo Fisher Scientific) supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) and sonicated 15s in an ultrasonic bath (XUBA3, Grant, United Kingdom). The protein concentrations, gel electrophoresis, proteins transfer, antibodies incubations, blot development and quantification were performed as described in Ref. [20]. Intensity values of related bands were normalized to β-actin housekeeping protein values. Representative blots of at least four independent experiments are shown.
Quantitative real-time PCR (qRT-PCR). As previously described in Ref. [20], the RNA was isolated using NucleoSpin® RNA Plus kit (Macherey-Nagel, Dueren, Germany) and quantified with a NanoDrop™ spectrophotometer (Thermo Fisher Scientific). The cDNA was synthesized from 1 μg total RNA with PrimeScript RT reagent kit (Takara, Shimogyō-ku, Kyoto, Japan) in the presence of random primers. The qPCRs were performed using SensiFAST SYBR Hi-ROX Kit (Meridian Bioscience, Cincinnati, OH, USA) on a StepOne Plus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). Each assay was performed in duplicates for each sample, and the GAPDH expression was used as an internal control. The relative expression ratio was calculated using the 2-ΔΔCt method. Representative qRT-PCRs of at least four independent experiments are shown. The pairs of primers used were: S1PR2 - Migration and Proliferation Assay. As previously described in Ref. [20] briefly, in the migration assays HCT116 ShCTR and HCT116 ShCyR61 cells were plated at 50,000 cells/well in a 96-well plate and incubated 24 h at 37 • C and 5% CO 2 to create a monolayer of cells. The WoundMaker from Essen BioScience (Ann Arbor, MI, USA) was used to create homogeneous wide wounds. In the proliferation assays HCT116 ShCTR and HCT116 ShCyR61 cells were seeded in a 96-well plate at 13, 400 cells/well. The cells from the migration and proliferation assays were then incubated in IncuCyte device (10× objective) (Essen Bioscience) and the confluence was recorded every 2 h by phase/contrast scanning for 48 h at 37 • C and 5% CO 2 . Images of at least four independent experiments were analyzed using the IncuCyte ZOOM 2018A software (Essen Bioscience).

Author contributions
KA: experimental design, experimentation, data collection, statistical analysis, data interpretation, figure preparation, manuscript writing; BL: experimentation, data collection; CS: acquiring grant support, supervision, experimental design, data interpretation, manuscript writing.

Declaration of competing interest
None.
CyR61 Promoter Assay. As previously described in Ref. [20], HCT116 cells were seeded one day before the assay in 96-well plates. Complete medium containing GYY4137 or Na 2 S 3 serial dilutions was added to the cells followed by an incubation of 48 h at 37 • C and 5% CO 2 . The cells were then detached and incubated in an optimal dilution of a LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit (Invitrogen, Thermo Fisher Scientific) for 15 min, on ice. The cells were then analyzed using a FACS Fortessa flow cytometer (BD Biosciences). Emission signals for mCherry (610/20) and Live/dead IR (780/60) were detected. Data were analyzed with FlowJo (v10.8.1) software. Data shown are representative of FACS from at least four independent experiments in triplicates.
ELISA. The secreted CyR61 proteins were quantified using a Human CYR61/CCN1 ELISA Kit (Abcam, ab213772) according to manufacturer's instructions. Data shown are representatives of ELISAs from at least four independent experiments in duplicates.
Statistical Analysis. Data are shown as mean ± SEM. One-way ANOVA with Dunnett's multiple comparisons test, Kruskal-Wallis with Dunn's multiple comparisons test, two-way ANOVA Sidak's multiple comparisons test and unpaired t-test were used to detect differences between groups. Statistically significant differences between these two groups are indicated by *p<0.05 or **p<0.01. Statistical calculations were performed using Graphpad Prism analysis software.