Research had conducted a survey with a sample population of the employees working in the information Technology section in Thailand of 400 people. The research hypothesis is the relationship of knowledge factor and the acceptance of cloud computing technology features (cost reduction, time-saving, flexibility, solving technological problems, security and privacy, and reasonable service fee). The statistics used for data analysis are descriptive statistics and inferential statistics (Chi-Square). The analysis of the hypothesis found that the usability knowledge do have significant relationship with the acceptance in technological features in every aspect. The most significant relationship is noticeable in the system usability knowledge and the reasonable service fee with the weak relationship in time-saving and flexibility. The research suggests the organization to start their implementations of cloud computing by educating their employees with the theoretical and practical part of the technology. When they have enough knowledge, this should lead them to the implementation and acceptance of the system more effectively.