Elsevier

Neuroscience

Volume 370, 1 February 2018, Pages 112-120
Neuroscience

Knockdown of zif268 in the Posterior Dorsolateral Striatum Does Not Enduringly Disrupt a Response Memory of a Rewarded T-Maze Task

https://doi.org/10.1016/j.neuroscience.2017.07.014Get rights and content
Under a Creative Commons license
open access

Highlights

  • Zif268 expression in the pDLS is not required for retrieval of habit-like memory.

  • Knockdown of pDLS Zif268 expression reduces habit-like memory restabilization.

  • Zif268 expression increased in the BLA after reward memory retrieval.

  • Despite extended T-Maze training rats did not use a S-R strategy.

Abstract

Under certain conditions pavlovian memories undergo reconsolidation, whereby the reactivated memory can be disrupted by manipulations such as knockdown of zif268. For instrumental memories, reconsolidation disruption is less well established. Our previous, preliminary data identified that there was an increase in Zif268 in the posterior dorsolateral striatum (pDLS) after expression of an instrumental habit-like 'response' memory, but not an instrumental goal-directed 'place' memory on a T-maze task. Here, the requirement for Zif268 in the reconsolidation of a response memory was tested by knockdown of Zif268, using antisense oligodeoxynucleotide infusion into the pDLS, at memory reactivation. Zif268 knockdown reduced response memory expression 72H, but not 7d later. Western blotting revealed a non-significant increase in Zif268 in the pDLS in rats using response memories, but there was no change in Zif268 expression in the hippocampus following retrieval of a place memory. Zif268 expression increased in the basolateral amygdala after memory reactivation whether a response or place strategy was used during reactivation. We propose that Zif268 expression in the basolateral amygdala may be linked to prediction error, generated by the absence of reward at reactivation. Taken together, these results suggest a complex role for Zif268 in the maintenance of instrumental memories.

Abbreviations

aDLS
anterior dorsolateral striatum
aDMS
anterior dorsomedial striatum
ANOVA
analysis of variance
A-O
‘action-outcome’
BLA
Basolateral Amygdala
IEG
Immediate Early Genes
NAc
Nucleus Accumbens
ODN
Oligodeoxynucleotides
pDLS
posterior dorsolateral striatum
pDMS
posterior dorsomedial striatum
S-R
‘stimulus-response’
Zif268
Zinc Finger Protein 225

Key words

zif268
memory
striatum
amygdala
hippocampus
T-Maze

Cited by (0)