Detailed analysis of size-separation of silver nanoparticles by density gradient centrifugation method

https://doi.org/10.1016/j.msec.2019.109817Get rights and content

Highlights

  • The Ag nanoparticles were synthesized in the range of 15–235 nm and stabilized by chitosan biopolymer.

  • The structure and chemical composition of the nanoparticles were fully characterized.

  • The silver nanoparticles were separated by density gradient centrifugation method.

  • The best separation was done with the gradient chosen at 10%, 20%, 30% and 40%.

Abstract

In this research, silver nanoparticles were synthesized by chemical precipitation method and stabilized by chitosan biopolymer in the range of 15 to 235 nm. Then, the silver nanoparticles were separated by density gradient centrifugation method at different gradients and centrifuged at various duration and speed. The best separation was done with the gradient chosen at 10%, 20%, 30% and 40% for a duration of 2 h at 6000 rpm. The UV–visible spectra demonstrated the proper synthesis of silver nanoparticles, while FTIR spectrum and XRD data revealed the structure of prepared Ag-NPs. The FESEM and TEM analysis were used to check for the exact shape and size of Ag-NPs, respectively. However, the separation of silver nanoparticles was assessed making use of DLS analysis.

Keywords

Silver nanoparticles
Density gradient centrifugation
Chitosan
Chemical precipitation
Size separation

Cited by (0)

View Abstract