Short communicationDifferentially expressed sequences from a cestode parasite reveals conserved developmental genes in platyhelminthes
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Acknowledgements
We would like to thank Adriana de Freitas Schuck, Christyanne Thomaz Martinkovics, and Roberta Alvares Campos for helping in sequencing cDNA clones, and Dr. Arthur Fett Neto for the critical reading of the manuscript. This work was supported by CNPq/Brazil, FAPERGS/Brazil, RTPD Network (SIDA, Sweden), and FAPESP/Brazil. C.V.B. was a recipient of a CAPES/Brazil pre-doctoral fellowship. F.K.R. was a recipient of a FAPERGS fellowship. M.H.B. was a recipient of a FAPESP pre-doctoral fellowship.
References (29)
Serologic and molecular diagnosis of zoonotic larval cestode infections
Parasitol Int
(2002)- et al.
Cestode parasites: application of in vivo and in vitro models for studies on the host-parasite relationship
Adv Parasitol
(2002) - et al.
Basic local alignment search tool
J Mol Biol
(1990) - et al.
Mi-2/NuRD: multiple complexes for many purposes
Biochim Biophys Acta
(2004) - et al.
Identification and characterization of SET, a nuclear phosphoprotein encoded by the translocation break point in acute undifferentiated leukemia
J Biol Chem
(1994) - et al.
Regulation of histone acetylation and transcription by INHAT, a human cellular complex containing the set oncoprotein
Cell
(2001) - et al.
Mesocestoides corti: a LIM-homeobox gene upregulated during strobilar development
Exp Parasitol
(2004) - et al.
Echinococcosis: disease, detection and transmission
Parasitology
(2003) - et al.
The new animal phylogeny: reliability and implications
Proc Natl Acad Sci USA
(2000) - et al.
Testing the new animal phylogeny: first use of combined large-subunit and small-subunit rRNA gene sequences to classify the protostomes
Mol Biol Evol
(2002)
Development of an animal model for neurocysticercosis: immune response in the central nervous system is characterized by a predominance of gamma delta T cells
J Immunol
Asexual multiplication of Mesocestoides tetrathyridia in laboratory animals
J Parasitol
In vitro segmentation induction of Mesocestoides corti (Cestoda) tetrathyridia
J Parasitol
Identifying differences in mRNA expression by representational difference analysis of cDNA
Nucl Acids Res
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