Short communicationQuantitation of HIV-1 RNA in dried blood and plasma spots
Section snippets
Acknowledgements
We thank Valérie Jauvin and Patricia Pinson for their help with the use of filter paper and helpful discussion. We thank all patients who participated in the study.
Funding: This work was funded by the French Ministry of Education and Research through the quadrennial contract of the EA2968.
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2013, Journal of Virological MethodsCitation Excerpt :These findings could be explained by the natural history of HIV-1 infection in children, characterised by high levels of plasmatic HIV-1 RNA viral load and rapid progress to AIDS and death in a context where PMTCT programmes lack performance (Stringer et al., 2010). HIV-1 RNA levels measured on plasma and DBS were strongly correlated which is consistent with others studies that have shown a high correlation between HIV-1 RNA levels on plasma and DBS with the Biocentric kit and other commercial kits (Johannessen et al., 2009; Lofgren et al., 2009; Reigadas et al., 2009; Viljoen et al., 2010; Kébé et al., 2011). The median plasmatic viral load level was in line with values obtained in other studies (5.1–5.6 log10 copies/ml) (Rouet et al., 2005, 2007; Viljoen et al., 2010; Kébé et al., 2011; Burgard et al., 2012).
Dried blood spots for monitoring HIV infection in Public Health Programs in developing countries
2013, Enfermedades Infecciosas y Microbiologia ClinicaComparison of HIV-1 RNA level estimated with plasma and DBS samples: A pilot study from India (South)
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2012, Journal of Virological MethodsCitation Excerpt :DBS and DPS have been shown to be useful means for specimen transportation and preservation for several RNA viruses (Chibo et al., 2005; Choi et al., 2009; De Swart et al., 2001; De Crignis et al., 2010; Desbois et al., 2009; Katz et al., 2002; Lenselink et al., 2009). They have been shown to support quantitative HIV viral load testing for monitoring patients on antiretroviral therapy (ART) (Andreotti et al., 2010; Garrido et al., 2009; Johannessen et al., 2009a; Leelawiwat et al., 2009; Lofgren et al., 2009; Marconi et al., 2009; Mbida et al., 2009; Mehta et al., 2009; Reigadas et al., 2009; Viljoen et al., 2010). However, DBS and DPS place constraints on the lower limit of detection (LOD) attainable and the dynamic range over which there is adequate quantitative discrimination (Andreotti et al., 2010; Johannessen et al., 2009a,b; Lofgren et al., 2009).
Dried blood spots versus plasma for the quantitation of HIV-1 RNA using a real-Time PCR, m2000rt assay
2012, Journal of Virological MethodsCitation Excerpt :Few studies have reported an over estimation of HIV-1 RNA levels in specimens with low-level viremia (below 5000 copies/mL). In this study, a larger difference between DBS and plasma HIV-1 RNA was noticed in the range of specimens below 3000 copies/mL, which remains consistent with two studies using the m2000rt platform (Marconi et al., 2009; Mbida et al., 2009; Lofgren et al., 2009) and with other reports using different viral load assays (Monleau et al., 2009; Reigadas et al., 2009; Waters et al., 2007). This repeated finding may be explained by the contribution of intracellular HIV-1 DNA and RNA which, by definition, is present in the DBS but not in the plasma counterpart.
HIV-1 and HCV detection in dried blood spots by SYBR Green multiplex real-time RT-PCR
2010, Journal of Virological Methods