Intrinsic aggregation propensity of the CsgB nucleator protein is crucial for curli fiber formation
Introduction
Amyloids are an after-effect of deposition of ordered protein fibrillar arrangements, known as amyloid fibrils (Chiti and Dobson, 2006). Amyloid fibrils are formed by otherwise soluble proteins or peptides that convert under certain conditions into insoluble fibrous aggregates (Dobson, 1999). Impressively, several proteins with important but otherwise unrelated functions have been associated with amyloid deposition, although they have neither sequence nor structural apparent similarities (Sipe et al., 2014). A large number of widespread diseases, such as AL (Amyloid Light-chain), AA (Amyloid A) or ATTR (Transthyretin-related) amyloidosis, neurodegenerative diseases (Alzheimer’s, Parkinson’s and Creutzfeldt-Jakob’s disease among others), type II diabetes and many more are a consequence of unrestrained deposition of amyloid causing tissue damage and degeneration (Sipe et al., 2014). In contrast, accumulated evidence has shown that occasionally organisms spanning from bacteria to humans exploit the extraordinary intrinsic properties of amyloids in order to support fundamental physiological processes (Iconomidou and Hamodrakas, 2008, Shewmaker et al., 2011). Typical examples of functional amyloid include the extracellular protective coats of several organisms (Iconomidou et al., 2000, Louros et al., 2013, Louros et al., 2014a, Louros et al., 2016b), the intracellular Pmel17 template which is utterly important for the biosynthesis of melanin (Fowler et al., 2006, Louros and Iconomidou, 2015, Louros et al., 2016a) and the formation of biofilms by gram negative bacteria (Hammar et al., 1995).
Bacteria are able to survive and colonize in a diverse variety of environments. Key feature to their resilience is their ability to grow in colonies and produce a complex matrix of extracellular polymeric substances (Donlan, 2002). Using this matrix, bacteria are able to sculpt three-dimensional structures, called biofilms, which shelter the inhabitants from environmental stress (Donlan, 2002). In the case of many Enterobacteriaceae such as Escherichia coli or Salmonella enterica, the major proteinaceous component of this matrix consists of fibers called curli that are involved in cell-surface and cell-cell contacts and adhesion (Barnhart and Chapman, 2006). Curli fibers exhibit characteristic properties of amyloid fibrils (Chapman et al., 2002) and are composed of two basic proteins; namely, a major structural subunit, known in E. coli as CsgA, and a minor subunit serving also as a nucleator protein, known as CsgB, both encoded by a common operon (Hammar et al., 1996, Hammar et al., 1995). The two proteins are of identical size (151aa) and comprise similar structural segments (Hammar et al., 1996). Specifically, CsgA and CsgB are built up of three regions, namely a signal peptide, an N-terminal chaperone-binding peptide segment and five consecutive repeat subunits, composed of 22–23 residues, which constitute the amyloid core of curli fibers (Hammer et al., 2007, Wang and Chapman, 2008). Each repeat is composed of a strand-loop-strand motif predicted to form two parallel β-sheets (Wang and Chapman, 2008, Wang et al., 2008).
Both proteins are capable of forming fibers in vitro, however detailed evidence indicates that CsgB forms fibers with a significantly faster rate (Hammar et al., 1996). Additionally, the aggregation kinetics of CsgA is accelerated in the presence of CsgB (Hammer et al., 2012). However, in vivo curli fibers can only be formed in the presence of both subunits (Barnhart and Chapman, 2006, Blanco et al., 2012, Hammer et al., 2012, Wang et al., 2007), accumulated at ratios approximately 20:1 (CsgA:CsgB) (Van Gerven et al., 2015, White et al., 2001). Furthermore, detailed immunoelectron microscopy studies have shown that CsgB can form short polymers on the cell surface in the absence of CsgA (Bian and Normark, 1997). Finally, CsgB has been proposed to be responsible for creating the initial oligomers/nuclei acting as templates and inducing the polymerization of CsgA, which self-assembles forming the major axis of the curli fiber (Hammer et al., 2007, Shu et al., 2012).
Experimental and theoretical evidence has indicated that amyloid formation is induced by specific short sequence regions of a polypeptide chain that are prone to aggregation, hence regulating the overall aggregation tendency of the protein (Lopez de la Paz and Serrano, 2004, Louros et al., 2015a, Louros et al., 2015b, Teng et al., 2012). In this work, we have identified six individual aggregation-potent regions of the minor curli subunit, by comparing the amyloidogenic profile of both CsgA and CsgB curli proteins of E. coli. Structural studies of peptide-analogues corresponding to the identified regions indicate their ability to self-assemble forming fibrils with characteristic amyloidogenic features. Our findings suggest that the ‘aggregation-prone’ segments could probably be responsible for the ability of CsgB to self-assemble in faster rates than CsgA, in vitro, and may also promote the nucleation capabilities of the former. Finally, based on our results, we propose a possible mechanism for both the in vivo and in vitro curli fiber self-assembly process, by attempting to shed some light on the vague and complicated nucleation process of curli fibers.
Section snippets
Aggregation propensity sequence analysis of CsgA and CsgB
Protein sequences of CsgA and CsgB from E. coli were obtained from Uniprot (Accession Numbers: P28307 and P0ABK7). Initially, the Sec-signal peptide and the 22-residue long N-terminal domain that precedes 5 imperfect repeats in both CsgA and CsgB (Fig. S1) were removed. In order to track down the aggregation propensity of both curli subunits, we implemented AMYLPRED2, a consensus aggregation propensity prediction tool, which was developed by our lab (Tsolis et al., 2013), on the remaining
CsgB exhibits increased aggregation propensity compared to CsgA
The aggregation potency of both the major and minor subunit of E. coli curli fibers was calculated and analyzed utilizing AMYLPRED2. Impressively, although the aforementioned sequences share relatively high sequence homology, significant differences emerge regarding their aggregation tendency. As the results of AMYLPRED2 clearly indicate, severely lower aggregation potency was predicted for CsgA in comparison to CsgB, since the former only presents segments with an extraneous minor aggregation
Funding
The research leading to these results has received partial funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under BioStruct-X (grant agreement N°283570). The infrastructure that the authors used for the present work is associated with the Greek National Research Infrastructure in Structural Biology, Instruct-EL.
Acknowledgements
We thank the Institute of Biology, Medicinal Chemistry and Biotechnology at National Hellenic Research Foundation for hospitality. We acknowledge the help of Dr. Evangelia Chrysina with the X-ray diffraction experiments. The help of Dr. George Baltatzis and Prof. Efstratios Patsouris and the use of the Morgagni Microscope at the 1st Department of Pathology, Medical School, University of Athens are also gratefully acknowledged. We also thank the University of Athens for support. Finally, the
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