Abdominal wall repair using a biodegradable scaffold seeded with cells
Section snippets
Cell culture
3T3 mouse fibroblasts were grown in vitro in 95% humified 5% CO2 chamber at 37°C. Growth medium consisted of Dulbecco's Modified Eagle's Medium with glutamine (Sigma, Germany) supplemented with 10% fetal bovine serum (Gibco, UK) and antibiotic solution (100 U/mL penicillin G sodium, 100 μg/mL gentamicin, 100 μg/mL streptomycin, and 5 μg/mL amphotericin, Sigma). Cells were cultured in 75-cm2 culture flasks (Corning) and passaged every 3 days using trypsin 1× solution (2.5 g trypsin per liter
Results
It took 7 days to achieve sufficient 3T3 cell number for further seeding. Cells were seeded 3 times on the scaffold until tightly covering was obtained. The adhesive properties of PGA fibers were excellent. Cells started to grow on PGA construct after the first seeding and covered the scaffold fibers within 3 days (Fig. 1). Medium was changed every 6 hours after the pH decrease was observed. No signs of scaffold degradation were noticed during the period of 3 days of tissue culture on 3D
Discussion
The complication rate after repair of large tissue defects strongly depends on applied reconstructive material. Wound infections, bowel fistulae, and repair failures can occur when synthetic materials were used for defect closure. Naturally derived materials are less susceptible to infection [4], [6], [7]. The strength of allogenic and xenogenic matrices decreases over time [9]. Authors tried to find compromise between good mechanical properties that belong to synthetic materials and graft
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