Letter to the editorSelf-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing
Section snippets
Acknowledgments
We thank Profs. Li-Jia Qu, Lizhong Xiong, and Guoliang Wang for providing plasmids. This work was supported by a National Transgenic Science and Technology Program (2016ZX08010002) to R.W. and a startup fund from the Huazhong Agricultural University.
References (17)
- et al.
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Trends Biotechnol.
(2013) - et al.
Engineering herbicide-resistant rice plants through CRISPR/Cas9-mediated homologous recombination of acetolactate synthase
Mol. Plant
(2016) - et al.
A single transcript CRISPR-Cas9 system for efficient genome editing in plants
Mol. Plant
(2016) - et al.
A versatile zero background T-vector system for gene cloning and functional genomics
Plant Physiol.
(2009) - et al.
Multiplex genome engineering using CRISPR/Cas systems
Science
(2013) - et al.
Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development
Proc. Natl. Acad. Sci. U. S. A.
(2015) - et al.
Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
J. Integr. Plant Biol.
(2014) - et al.
Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9
J. Clin. Invest.
(2014)
There are more references available in the full text version of this article.
Cited by (69)
ROS-stimulated protein lysine acetylation is required for crown root development in rice
2023, Journal of Advanced ResearchCitation Excerpt :Seedlings (seven days old) were treated with 1 mM KI and 0.5 mM H2O2 for 7 d, by adding the chemicals directly to the hydroponic culture solution. The design of sgRNA for the CRISPR/Cas9 system and transgenic vectors were constructed as previously described [23]. CRISPR-P2.0[24] was used to design sgRNAs of PRX genes.
CRISPR/Cas9 technology and its application in horticultural crops
2022, Horticultural Plant JournalSgRNA engineering for improved genome editing and expanded functional assays
2022, Current Opinion in Biotechnology
© 2017 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.