Elsevier

Journal of Functional Foods

Volume 72, September 2020, 104085
Journal of Functional Foods

Ameliorative effect of low molecular weight peptides from the head of red shrimp (Solenocera crassicornis) against cyclophosphamide-induced hepatotoxicity in mice

https://doi.org/10.1016/j.jff.2020.104085Get rights and content
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Highlights

  • Low molecular-weight peptides were prepared from Solenocera crassicornis head.

  • SCHPs-F1 ameliorated hepatotoxicity induced by cyclophosphamide in mice.

  • SCHPs-F1 restored endogenous antioxidants levels by activating the Nrf2 signal.

  • SCHPs-F1 suppressed hepatic inflammation through NF-κB signal pathway.

  • SCPHs-F1 relieved CTX hepatotoxicity as functional component.

Abstract

Our study was aimed at investigating the protective effect of low molecular weight peptides (SCHPs-F1) from red shrimp (Solenocera crassicornis) head against cyclophosphamide-induced hepatotoxicity in mice. We found that SCHPs-F1 treatment dose-dependently normalized the biochemical markers, hepatic index, and total CYP450 enzyme content in CTX-induced hepatotoxicity in mice. SCHPs-F1 also ameliorated the CTX-mediated structural disorders of the hepatic tissue. Western blot results suggested that SCHPs-F1 significantly restored the levels of endogenous antioxidants (CAT, T-AOC, GSH-Px, SOD and MDA levels) through activating the Nrf2 signal by upregulating the expression of GCLM, HO-1, and NQO-1. Moreover, SCHPs-F1 improved the CTX-induced hepatotoxicity by inhibiting of NF-κB signal responses and down-regulating the expression of the inflammatory factors (IL-1β, IL-6, IFN-γ and TNF-α). These findings suggest that SCHPs-F1 can regulate Nrf2 and NF-κB signaling pathways and reduce the oxidative stress and inflammation in CTX-induced hepatotoxicity. Overall, SCHPs-F1 are value-added food ingredients for alleviating CTX-induced hepatotoxicity.

Keywords

Solenocera crassicornis
Cyclophosphamide
Hepatotoxicity
Low molecular weight peptides

Abbreviations

CTX
Cyclophosphamide
MW
Molecular Weight
ROS
Reactive oxygen species
ALT
Alanine aminotransferase
AST
Aspartate aminotransferase
AKP
Alkaline phosphatase
CYP 450
Cytochrome P450
Nrf2
Nuclear factor erythroid-2-related factor 2
Keap1
Kelch-like ECH-associated protein 1
CAT
Catalase
T-AOC
Total antioxidant capacity
GSH-Px
Glutathione peroxidase
SOD
Superoxide Dismutase
MDA
Malondialdehyde
GCLM
Glutamate cysteine ligase modifier subunit
HO-1
Heme oxygenase 1
NQO-1
NADPH Quinone acceptor Oxidoreductase 1
NF-κB
Nuclear factor kappa-B
IKK
Inhibitor of nuclear factor kappa-B kinase
IκB
Inhibitor of NF-κB
6IL-1β, 6
Interleukin-1β
IFN-γ
Interferon-γ
TNF-α
Tumor necrosis factor-α

Cited by (0)

1

These authors contributed equally to this work.