Isolation and partial characterization of a neutral polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru and its antioxidant and immunomodulatory activities

doi:10.1016/j.jff.2013.11.007

Journal of Functional Foods

Volume 6, January 2014, Pages 410-418

https://doi.org/10.1016/j.jff.2013.11.007 Get rights and content

Highlights

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A neutral polysaccharide MP-1 was purified from Mosla chinensis Maxim. cv. Jiangxiangru.
•
The average molecular weight and monosaccharide of MP-1 composition was detected.
•
Partial structural characterizations of MP-1 were elucidated by methylation analysis.
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MP-1 exhibited a dose-dependent enhancement in antioxidant assays in this study.
•
Immunomodulatory effects of MP-1 were assessed in vitro.

Abstract

A neutral polysaccharide MP-1 was isolated and purified from Mosla chinensis Maxim. cv. Jiangxiangru. The average molecular weight was 587.53 kDa and it was composed of glucose, galactose, arabinose, mannose and rhamnose in a molar ratio of 16.02:10.01:4.92:4.62:2.23, and trace of fucose. Partial characterization of MP-1 was elucidated by methylation analysis. MP-1 was composed of 1,4-Glcp (31.76%), followed by 1,2-Rhap (17.11%), 1,2,4-Rhap (6.47%), 1,3,4-Galp (5.05%), 1,4-Manp (3.61%), 1,2,4-Manp (2.55%), 1,5-Araf (3.10%) and 1,4,6-Glcp (1.53%). The branching points were located at O-2, O-4 or O-6 with α-Araf (4.01%), α-Rhap (4.31%), α-Fucp (2.39%), β-Galp (11.02%) and β-Glcp (4.04%) as terminal residues. MP-1 exhibited a dose-dependent enhancement in antioxidant assays in this study. Immunomodulatory effects of MP-1 were assessed by splenocyte proliferation test and nitric oxide production of mouse peritoneal macrophages.

Introduction

Polysaccharides are macromolecules that are widely distributed in nature. In the last few decades, polysaccharides have been the focus of much attention in the fields of biochemistry and medicine, including antiviral, antitumour (Zhang et al., 2012), immunostimulating (Wu, Liaw, & Pan, 2013), antiinflammatory, anticomplementary, anticoagulant, and hypoglycaemic products (Capek et al., 2003). Many polysaccharides extracted from animals, plants, and microorganisms exhibit strong antioxidant properties and have been explored as novel antioxidants (Ge et al., 2009, Hu et al., 2003, Ramarahnam et al., 1995, Yuan et al., 2008). Moreover, previous studies have indicated that such activities might be closely related to the structural characteristics of polysaccharides (Chen et al., 2008, Wang and Luo, 2007). However, not all polysaccharides possess antioxidant activities. It was reported that the antioxidant capacities of polysaccharides usually depend on the sugar unit, glycosidic bonds, degree of branching and the conformation of the polysaccharide (Lu, Wang, Hu, Huang, & Wang, 2008).

Mosla chinensis Maxim. cv. Jiangxiangru is a popular medicinal vegetable with an early historical record in the ancient medical book “MING YI BIE LU” (China Pharmacopoeia Committee., 2005). M. chinensis Maxim. cv. Jiangxiangru belongs to Labiatae family, and is widely distributed in southern parts of China, Vietnam, India, as well as Japan. As a potential functional food with phamaceutical value, it is commonly taken for the treatment of many diseases, such as cold, fever, diarrhea, dysentery, digestion disorder, vomiting, stroke and edema (Liu, Lee, Wang, & Du, 2007). The present study aims to investigate partial characterization of a purified neutral polysaccharide MP-1 isolated from M. chinensis Maxim. cv. Jiangxiangru, and to evaluate its antioxidant and immunomodulatory activities in vitro.

Section snippets

Samples and chemicals

M. chinensis Maxim. cv. Jiangxiangru was bought from Zhangshu, Jiangxi, China. The sample was sliced, ground into powder, and passed through a 20-mesh sieve (0.83 mm) before extraction.

DEAE-52 cellulose was purchased from Whatman (Maidstone, UK). Sephacryl™ S-400 High Resolution (HR) was from GE Healthcare Bio-Sciences Corp. (Salt Lake City, Utah, USA). Trifluoroacetic acid (TFA) was from Aladdin Chemical Reagent Co., Ltd (Shanghai, China). Dextrans with different molecular weights were

Isolation and purification of polysaccharides

CMP isolated from M. chinensis Maxim. cv. Jiangxiangru was first eluted through a DEAE-52 anion-exchange column to yield two peaks (A and B) (Fig. 1A). Peak B (the main peak) was further purified by Sephacryl™ S-400 HR size-exclusion chromatography based on the molecular size. Elution curve was shown as Fig. 1B. Eluation product was collected, dialyzed and lyophilized to obtain a purified polysaccharide, named as MP-1, and the yield of which was 0.53% of the plant. Fig. 1C showed a single and

Conclusions

In present study, a purified neutral polysaccharide MP-1 was successfully isolated and characterized from M. chinensis Maxim. cv. Jiangxiangru. Monosaccharide composition analysis indicated that MP-1 was a heteropolysaccharide and consisted of glucose, galactose, arabinose, mannose and rhamnose in a molar ratio of 16.02:10.01:4.92:4.62:2.23, and trace of fucose. The linked patterns between these monosaccharide residues were elucidated by methylation analysis.

The antioxidant and immunomodulatory

Declaration of interest statement

There are no conflicts of interest. The author and co-authors are responsible for the content and writing of this paper.

Acknowledgements

The authors are grateful for the financial supports by National High-tech R&D Program of China (863 Program) (2013AA102102), National Natural Science Foundation of China (31130041), National Key Technology R&D Program of China (2012BAD33B06), the Program for New Century Excellent Talents in University (NCET-12-0749), Science and Technology Project of Jiangxi Provincial Department of Science and technology (20121BBF60040), the Project of Science and Technology of Jiangxi Provincial Education

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Isolation and partial characterization of a neutral polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru and its antioxidant and immunomodulatory activities

Highlights

Abstract

Introduction

Section snippets

Samples and chemicals

Isolation and purification of polysaccharides

Conclusions

Declaration of interest statement

Acknowledgements

Food Chemistry

International Journal of Biological Macromolecules

Food Chemistry

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Archives of Biochemistry and Biophysics

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Phytochemistry